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Original article
Bin Liu
Rice Research Institute, Guangdong Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3736-7522
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Although panicle blast is more destructive to yield loss than leaf blast in rice, the cloned genes that function in panicle blast resistance is still very limited and the molecular mechanisms underlying panicle blast resistance remain largely unknown.
Results
In the present study, we have confirmed that the three OXO genes, OsOXO2, OsOXO3 and OsOXO4 from a blast-resistant cultivar BC10 function in panicle blast resistance in rice. The expression of OsOXO2, OsOXO3 and OsOXO4 were induced by panicle blast inoculation. Subcellular localization analysis revealed that the three OXO genes are all localized in the cell wall. Simultaneous silencing of OsOXO2, OsOXO3 and OsOXO4 decreased rice resistance to panicle blast, whereas the OsOXO2, OsOXO3 and OsOXO4 overexpression rice plants showed enhanced panicle blast resistance. More H2O2 and higher expression levels of PR genes were observed in the overexpressing plants than in the control plants, while the gene silencing plants exhibited less H2O2 and lower expression levels of PR genes compared to the control plants. Moreover, phytohormone treatment and the gene expression analysis showed that panicle blast resistance mediated by the three OXO genes was associated with the induction of JA and ABA signaling pathways but suppression of SA signaling pathway.
Conclusion
OsOXO2, OsOXO3 and OsOXO4 positively regulate panicle blast resistance in rice. The OXO genes could modulate the accumulation of H2O2 and expression levels of PR gene in plants. Moreover, the OXO genes mediated panicle blast resistance is associated with the activation of JA and ABA signaling pathways but suppression of the SA signaling pathway.
Keywords
Rice (Oryza sativa L.), Panicle blast, OXO (Oxalate oxidase)
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View the published article at Rice.
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Review #3 received
Received 16 Jan, 2021
Editorial decision: Major revision
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Received 15 Jan, 2021
Review #1 received
Received 15 Jan, 2021
Reviewer #3 agreed
On 27 Dec, 2020
Reviewer #2 agreed
On 26 Dec, 2020
Reviewers invited
Invitations sent on 26 Dec, 2020
Reviewer #1 agreed
On 26 Dec, 2020
Editor assigned
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On 24 Dec, 2020
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On 24 Dec, 2020
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Rice.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original article
Bin Liu
Rice Research Institute, Guangdong Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3736-7522
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Rice.
Version 1
Posted 31 Dec, 2020
No community comments so far
Review #3 received
Received 16 Jan, 2021
Editorial decision: Major revision
On 16 Jan, 2021
Review #2 received
Received 15 Jan, 2021
Review #1 received
Received 15 Jan, 2021
Reviewer #3 agreed
On 27 Dec, 2020
Reviewer #2 agreed
On 26 Dec, 2020
Reviewers invited
Invitations sent on 26 Dec, 2020
Reviewer #1 agreed
On 26 Dec, 2020
Editor assigned
On 24 Dec, 2020
Submission checks complete
On 24 Dec, 2020
Editor invited
On 24 Dec, 2020
First submitted
On 23 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Rice.
Background
Although panicle blast is more destructive to yield loss than leaf blast in rice, the cloned genes that function in panicle blast resistance is still very limited and the molecular mechanisms underlying panicle blast resistance remain largely unknown.
Results
In the present study, we have confirmed that the three OXO genes, OsOXO2, OsOXO3 and OsOXO4 from a blast-resistant cultivar BC10 function in panicle blast resistance in rice. The expression of OsOXO2, OsOXO3 and OsOXO4 were induced by panicle blast inoculation. Subcellular localization analysis revealed that the three OXO genes are all localized in the cell wall. Simultaneous silencing of OsOXO2, OsOXO3 and OsOXO4 decreased rice resistance to panicle blast, whereas the OsOXO2, OsOXO3 and OsOXO4 overexpression rice plants showed enhanced panicle blast resistance. More H2O2 and higher expression levels of PR genes were observed in the overexpressing plants than in the control plants, while the gene silencing plants exhibited less H2O2 and lower expression levels of PR genes compared to the control plants. Moreover, phytohormone treatment and the gene expression analysis showed that panicle blast resistance mediated by the three OXO genes was associated with the induction of JA and ABA signaling pathways but suppression of SA signaling pathway.
Conclusion
OsOXO2, OsOXO3 and OsOXO4 positively regulate panicle blast resistance in rice. The OXO genes could modulate the accumulation of H2O2 and expression levels of PR gene in plants. Moreover, the OXO genes mediated panicle blast resistance is associated with the activation of JA and ABA signaling pathways but suppression of the SA signaling pathway.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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