This preprint is under consideration at European Journal of Medical Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Research
Yunshan Chen
Guangzhou Women and Children's Medical Center
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5134-8670
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This work is licensed under a CC BY 4.0 License. Read Full License
Objective: In intractable postpartum hemorrhage(IPPH), uterotonic lost efficacy in myometrum contraction formation. While the key target in uterotonic, OTR(oxytocin receptor) and internal environment in myometrum might have some changes when IPPH.
Methods: Three groups of myometrial tissue at different contraction states were collected:(1) term not at labour (TNL) group(10 cases); (2) term at labour (TAL) group(10 cases);(3) intractable postpartum hemorrhage(IPPH) group(10 cases).Expressions of OTR and internal environment markers(ASIC1a ,HIF1α, GlUT, FATP and apoptosis ) were compared in 3 groups by Immunoreactivity Scores method.
Results: The expression of OTR in myometrium in IPPH group was 100% negative ,compared to 30%,20% in TNL and TAL group(p < 0.05).70% positive HIF-1α expression in TNL group increased to 80% in TAL group,but decreased to 20% in IPPH group(P<0.05). While the positive expressions of ASIC1a, FATP and GLUT in three groups all had no significant difference.But the apoptosis related markers Bax,caspase-3 and caspase-9 positive expressions in IPPH were significantly all decreased(10%,10%,0%) compared to TNL and TAL group,compared to a high positive expression of Bcl-2 in 3 groups.
Conclusion: OTR expression and HIF-1α self-protection might be lost in myometrium of IPPH, which could lead to uterotonic lost efficacy and conservative treatment failure.
Keywords
intractable postpartum hemorrhage(IPPH), lost OTR expression, HIF-1α self-protection, myometrium
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This preprint is under consideration at European Journal of Medical Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Yunshan Chen
Guangzhou Women and Children's Medical Center
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5134-8670
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 05 Jan, 2021
No community comments so far
Submission checks complete
On 28 Dec, 2020
Editor assigned
On 27 Dec, 2020
Editor invited
On 27 Dec, 2020
First submitted
On 26 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Medical Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective: In intractable postpartum hemorrhage(IPPH), uterotonic lost efficacy in myometrum contraction formation. While the key target in uterotonic, OTR(oxytocin receptor) and internal environment in myometrum might have some changes when IPPH.
Methods: Three groups of myometrial tissue at different contraction states were collected:(1) term not at labour (TNL) group(10 cases); (2) term at labour (TAL) group(10 cases);(3) intractable postpartum hemorrhage(IPPH) group(10 cases).Expressions of OTR and internal environment markers(ASIC1a ,HIF1α, GlUT, FATP and apoptosis ) were compared in 3 groups by Immunoreactivity Scores method.
Results: The expression of OTR in myometrium in IPPH group was 100% negative ,compared to 30%,20% in TNL and TAL group(p < 0.05).70% positive HIF-1α expression in TNL group increased to 80% in TAL group,but decreased to 20% in IPPH group(P<0.05). While the positive expressions of ASIC1a, FATP and GLUT in three groups all had no significant difference.But the apoptosis related markers Bax,caspase-3 and caspase-9 positive expressions in IPPH were significantly all decreased(10%,10%,0%) compared to TNL and TAL group,compared to a high positive expression of Bcl-2 in 3 groups.
Conclusion: OTR expression and HIF-1α self-protection might be lost in myometrium of IPPH, which could lead to uterotonic lost efficacy and conservative treatment failure.
Figure 1
Figure 2
Figure 3
Figure 4
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