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Research Article
Martín Graziano
CONICET - Universidad de Buenos Aires. Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA)
Corresponding Author
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Escherichia coli dynamics in urban watersheds are affected by a complex balance among external inputs, niche modulation and genetic variability. To explore the ecological processes influencing E. coli spatial patterns, we analyzed its abundance and phylogenetic structure in water samples from a stream network with heterogeneous urban infrastructure and environmental conditions. Our results showed that environmental and infrastructure variables, such as macrophyte coverage, DIN and sewerage cover, mostly explained E. coli abundance, with a negative relationship between abundance and phylogroup richness. Moreover, main generalist phylogroups A and B1 were found in high proportion, which, together with an observed negative relationship between E. coli abundance and phylogroup richness suggests that their dominance is due to competitive exclusion. Low-frequency taxa were associated with sites of higher ecological disturbance, mainly involving simplified habitats, higher drainage infrastructure and septic tank density. In addition to the strong positive relationship between phylogroup richness and evenness, the occurrence of these phylogroups would be associated with increased facilitated dispersal. Nutrients also contributed to explaining phylogroup distribution. Our study highlights the differential contribution of distinct ecological processes to the patterns of E. coli in an urban watershed, which is useful for the monitoring and management of fecal pollution.
Keywords
DIN, phylogroup richness, Escherichia coli
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View the published article at Scientific Reports.
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Posted 31 Dec, 2020
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Editorial decision: Major revision
On 21 May, 2021
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Received 03 May, 2021
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On 27 Apr, 2021
Reviewers agreed
On 11 Apr, 2021
Reviewers invited
Invitations sent on 22 Jan, 2021
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On 22 Jan, 2021
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On 29 Dec, 2020
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On 29 Dec, 2020
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On 28 Dec, 2020
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See the published version of this preprint at Scientific Reports.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Martín Graziano
CONICET - Universidad de Buenos Aires. Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA)
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Scientific Reports.
Version 1
Posted 31 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 21 May, 2021
Reviews received
Received 06 May, 2021
Reviews received
Received 03 May, 2021
Reviewers agreed
On 27 Apr, 2021
Reviewers agreed
On 11 Apr, 2021
Reviewers invited
Invitations sent on 22 Jan, 2021
Editor assigned
On 22 Jan, 2021
Editor invited
On 29 Dec, 2020
Submission checks complete
On 29 Dec, 2020
First submitted
On 28 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Scientific Reports.
Escherichia coli dynamics in urban watersheds are affected by a complex balance among external inputs, niche modulation and genetic variability. To explore the ecological processes influencing E. coli spatial patterns, we analyzed its abundance and phylogenetic structure in water samples from a stream network with heterogeneous urban infrastructure and environmental conditions. Our results showed that environmental and infrastructure variables, such as macrophyte coverage, DIN and sewerage cover, mostly explained E. coli abundance, with a negative relationship between abundance and phylogroup richness. Moreover, main generalist phylogroups A and B1 were found in high proportion, which, together with an observed negative relationship between E. coli abundance and phylogroup richness suggests that their dominance is due to competitive exclusion. Low-frequency taxa were associated with sites of higher ecological disturbance, mainly involving simplified habitats, higher drainage infrastructure and septic tank density. In addition to the strong positive relationship between phylogroup richness and evenness, the occurrence of these phylogroups would be associated with increased facilitated dispersal. Nutrients also contributed to explaining phylogroup distribution. Our study highlights the differential contribution of distinct ecological processes to the patterns of E. coli in an urban watershed, which is useful for the monitoring and management of fecal pollution.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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