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Article
Transcriptional knockout of steroidogenic factor 1 in vivo in Oreochromis niloticus increased weight and suppressed gonad development using antisense RNA
Jun Qiang
Chinese Academy of Fishery Sciences Freshwater Fisheries Research Center
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4718-6618
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This work is licensed under a CC BY 4.0 License. Read Full License
Steroidogenic factor 1 (sf1) is an important regulator of gonad development and function in mammals. However, study of sf1 in fish is limited to cloning and expression and in vitro experiments. Using antisense RNA we knockout transcription of the sf1 gene in Nile tilapia Oreochromis niloticus, and obtain experimental fish in vivo. We demonstrate that antisense RNA can silence sf1 transcription and protein expression, and report suppression of sf1 transcription to affect gonad development and external genitalia formation in Nile tilapia. We also report disfunction of retinal metabolism and fatty acid metabolism to be important causes of weight gain and gonad abnormality with sf1 suppression. The feasibility of using antisense RNA for gene editing in fish is verified, and a new way of studying gene function and performing biological breeding is presented.
Keywords
Steroidogenic factor 1 (sf1), Nile tilapia, experimental fish, gene function, biological breeding
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- SupplementaryTable.docx
Supplementary Tables 1
- SupplementaryFigs.docx
Supplementary Figs. 1,2 and 3
- SourceDatafemale.xlsx
Source Data 1-female
- SourceDatamale.xlsx
Source Data 2-male
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This is a preprint. It has not completed peer review.
Article
Transcriptional knockout of steroidogenic factor 1 in vivo in Oreochromis niloticus increased weight and suppressed gonad development using antisense RNA
Jun Qiang
Chinese Academy of Fishery Sciences Freshwater Fisheries Research Center
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4718-6618
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 Jan, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Developmental Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Steroidogenic factor 1 (sf1) is an important regulator of gonad development and function in mammals. However, study of sf1 in fish is limited to cloning and expression and in vitro experiments. Using antisense RNA we knockout transcription of the sf1 gene in Nile tilapia Oreochromis niloticus, and obtain experimental fish in vivo. We demonstrate that antisense RNA can silence sf1 transcription and protein expression, and report suppression of sf1 transcription to affect gonad development and external genitalia formation in Nile tilapia. We also report disfunction of retinal metabolism and fatty acid metabolism to be important causes of weight gain and gonad abnormality with sf1 suppression. The feasibility of using antisense RNA for gene editing in fish is verified, and a new way of studying gene function and performing biological breeding is presented.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.