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Research Article
Eleni Voukali
Charles University
Corresponding Author
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Cerebrospinal fluid (CSF) proteins regulate neurogenesis, brain homeostasis and participate in signalling during neuroinflammation. Even though birds represent valuable models for constitutive adult neurogenesis, current proteomic studies of the avian CSF are limited to chicken embryos. Here we use liquid chromatography–tandem mass spectrometry (nLC-MS/MS) to explore the proteomic composition of CSF and plasma in adult chickens (Gallus gallus) and evolutionarily derived parrots: budgerigar (Melopsittacus undulatus) and cockatiel (Nymphicus hollandicus). Because cockatiel lacks a complete genome information, we compared the cross-species protein identifications using the reference proteomes of three model avian species: chicken, budgerigar and zebra finch (Taeniopygia guttata) and found the highest identification rates when mapping against the phylogenetically closest species, the budgerigar. In total, we identified 483, 641 and 458 unique proteins consistently represented in the CSF and plasma of all chicken, budgerigar and cockatiel conspecifics, respectively. Comparative pathways analyses of CSF and blood plasma then indicated clusters of proteins involved in neurogenesis, neural development and neural differentiation overrepresented in CSF in each species. This study provides the first insight into the proteomics of adult avian CSF and plasma and brings novel evidence supporting the adult neurogenesis in birds.
Keywords
Cerebrospinal fluid (CSF), brain homeostasis, nLC-MS/MS, Melopsittacus undulatus
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This is a list of supplementary files associated with this preprint. Click to download.
- Supplementarymaterial.pdf
• Supplementary material (Figures S1, S2, S3, S4, S5, S6, S7, S8, S9, S10).
- SupplementaryTableS1.nLCMSMSoutputfromdifferentmappingapproaches.xlsx
• Supplementary Table S1. nLC-MS/MS output from different mapping approaches.
- SupplementaryTableS2.CoreproteomelistsforCSFandPL.xlsx
• Supplementary Table S2. Core proteomes of CSF and PL.
- SupplementaryTableS3.GOClassificationandPathwayanalysesofCSFandPL.xlsx
• Supplementary Table S3. GO Classifications and Pathway analyses of CSF and PL.
- SupplementaryTableS4.DifferentiallyrepresentedproteinsinCSFvsplasma.xlsx
• Supplementary Table S4. Differentially represented proteins in CSF vs plasma.
- SupplementaryTableS5.GSEAresults.xlsx
• Supplementary Table S5. GSEA results.
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CURRENT STATUS: Published
View the published article at Scientific Reports.
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No community comments so far
Editorial decision: Major revision
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Editor invited
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A new preprint design is coming!
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See the published version of this preprint at Scientific Reports.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Eleni Voukali
Charles University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Scientific Reports.
Version 1
Posted 05 Jan, 2021
No community comments so far
Editorial decision: Major revision
On 22 Jan, 2021
Reviews received
Received 08 Jan, 2021
Reviewers agreed
On 01 Jan, 2021
Reviewers invited
Invitations sent on 01 Jan, 2021
Editor assigned
On 01 Jan, 2021
Editor invited
On 01 Jan, 2021
Submission checks complete
On 31 Dec, 2020
First submitted
On 30 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Scientific Reports.
Cerebrospinal fluid (CSF) proteins regulate neurogenesis, brain homeostasis and participate in signalling during neuroinflammation. Even though birds represent valuable models for constitutive adult neurogenesis, current proteomic studies of the avian CSF are limited to chicken embryos. Here we use liquid chromatography–tandem mass spectrometry (nLC-MS/MS) to explore the proteomic composition of CSF and plasma in adult chickens (Gallus gallus) and evolutionarily derived parrots: budgerigar (Melopsittacus undulatus) and cockatiel (Nymphicus hollandicus). Because cockatiel lacks a complete genome information, we compared the cross-species protein identifications using the reference proteomes of three model avian species: chicken, budgerigar and zebra finch (Taeniopygia guttata) and found the highest identification rates when mapping against the phylogenetically closest species, the budgerigar. In total, we identified 483, 641 and 458 unique proteins consistently represented in the CSF and plasma of all chicken, budgerigar and cockatiel conspecifics, respectively. Comparative pathways analyses of CSF and blood plasma then indicated clusters of proteins involved in neurogenesis, neural development and neural differentiation overrepresented in CSF in each species. This study provides the first insight into the proteomics of adult avian CSF and plasma and brings novel evidence supporting the adult neurogenesis in birds.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- Supplementarymaterial.pdf
• Supplementary material (Figures S1, S2, S3, S4, S5, S6, S7, S8, S9, S10).
- SupplementaryTableS1.nLCMSMSoutputfromdifferentmappingapproaches.xlsx
• Supplementary Table S1. nLC-MS/MS output from different mapping approaches.
- SupplementaryTableS2.CoreproteomelistsforCSFandPL.xlsx
• Supplementary Table S2. Core proteomes of CSF and PL.
- SupplementaryTableS3.GOClassificationandPathwayanalysesofCSFandPL.xlsx
• Supplementary Table S3. GO Classifications and Pathway analyses of CSF and PL.
- SupplementaryTableS4.DifferentiallyrepresentedproteinsinCSFvsplasma.xlsx
• Supplementary Table S4. Differentially represented proteins in CSF vs plasma.
- SupplementaryTableS5.GSEAresults.xlsx
• Supplementary Table S5. GSEA results.
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