Fibrogenesis imperfecta ossium (FIO) is a rare, metabolic bone disease clinically characterized by generalized bone pain and fragility fractures in both the axial and appendicular skeleton. The etiology of the disease is unknown and the pathogenesis is poorly understood. In this disorder, collagen arrangement in the bone is disorganized , and causes abnormalities in the organic bone matrix. Genetic cues causing this defect in collagen arrangement are largely undetermined. Microarray analysis showed remarkable changes in gene expression , when we compared gene expression of FIO with the control. Differential gene expression analysis revealed 50 gene signatures, which can be used to organize novel ways to diagnose and treat FIO patients.