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Lin Wang
University of Pennsylvania
Corresponding Author
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Direct assessment of human placental blood oxygenation can provide valuable information about placental function and, potentially, detect dysfunction. Currently however, no bedside tools exist for non-invasive monitoring of placental oxygenation. Here we report a continuous, non-invasive in vivo method to probe placental oxygen hemodynamics using deep penetrating Frequency Domain Diffuse Optical Spectroscopy (FD-DOS) with concurrent ultrasound (US) imaging. This multi-modal instrument facilitates assessment of placental oxygenation properties from image reconstruction algorithms that integrate anatomical US information about layer morphology with information from optics about functional hemodynamics. Tissue phantom experiments, simulations, and human subject studies validate the approach and demonstrate sensitivity to placental tissue located £ 5 cm below the surface. In a pilot study (n=24), human placental oxygen hemodynamics are measured non-invasively during maternal hyperoxia. Initial results suggest placental response to maternal hyperoxia may serve as a tool to detect placenta-related adverse pregnancy outcome and maternal vascular malperfusion of placenta, weeks before delivery.
Keywords
placental blood oxygenation, Frequency Domain Diffuse Optical Spectroscopy, ultrasound
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There is NO Competing Interest.
This is a list of supplementary files associated with this preprint. Click to download.
- P35V3Data.zip
Dataset1
- ExtendedDataFigure1Noninvasiveinvivoopticalmonitoringofhumanplacentaloxygenation.jpg
Detailed schematic of custom heterodyne FD-DOS instrument.
- ExtendedDataFigure2Noninvasiveinvivoopticalmonitoringofhumanplacentaloxygenation.jpg
Placental hemoglobin properties during stability test measurements and maternal left tilt experiment. (a) Mean (S.D.) of placental 〖StO〗_2, [〖Hb〗_T ], [〖HbO〗_2 ] versus gestational stage (GA) derived from the stability test measurements (error bar indicates S.D.). (b) Placental 〖StO〗_2, [〖Hb〗_T ], [〖HbO〗_2 ] of three individual subjects before/after maternal left tilt (error bar indicates S.D.).
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This preprint is under consideration at a Nature Portfolio Journal. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Article
Lin Wang
University of Pennsylvania
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 14 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Direct assessment of human placental blood oxygenation can provide valuable information about placental function and, potentially, detect dysfunction. Currently however, no bedside tools exist for non-invasive monitoring of placental oxygenation. Here we report a continuous, non-invasive in vivo method to probe placental oxygen hemodynamics using deep penetrating Frequency Domain Diffuse Optical Spectroscopy (FD-DOS) with concurrent ultrasound (US) imaging. This multi-modal instrument facilitates assessment of placental oxygenation properties from image reconstruction algorithms that integrate anatomical US information about layer morphology with information from optics about functional hemodynamics. Tissue phantom experiments, simulations, and human subject studies validate the approach and demonstrate sensitivity to placental tissue located £ 5 cm below the surface. In a pilot study (n=24), human placental oxygen hemodynamics are measured non-invasively during maternal hyperoxia. Initial results suggest placental response to maternal hyperoxia may serve as a tool to detect placenta-related adverse pregnancy outcome and maternal vascular malperfusion of placenta, weeks before delivery.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
There is NO Competing Interest.
This is a list of supplementary files associated with this preprint. Click to download.
- P35V3Data.zip
Dataset1
- ExtendedDataFigure1Noninvasiveinvivoopticalmonitoringofhumanplacentaloxygenation.jpg
Detailed schematic of custom heterodyne FD-DOS instrument.
- ExtendedDataFigure2Noninvasiveinvivoopticalmonitoringofhumanplacentaloxygenation.jpg
Placental hemoglobin properties during stability test measurements and maternal left tilt experiment. (a) Mean (S.D.) of placental 〖StO〗_2, [〖Hb〗_T ], [〖HbO〗_2 ] versus gestational stage (GA) derived from the stability test measurements (error bar indicates S.D.). (b) Placental 〖StO〗_2, [〖Hb〗_T ], [〖HbO〗_2 ] of three individual subjects before/after maternal left tilt (error bar indicates S.D.).
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