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Effect of Dietary Amylose/amylopectin Ratio on Antioxidant Ability and Amino Acid Metabolism of Liver in Weaned Pigs Challenged with Lipopolysaccharide
huansheng yang
Hunan Normal University; Hengyang Normal University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1164-5771
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: Antioxidant function could be regulated by resistant starch supplementation in rats with kidney disease. It is controversial whether antioxidant ability could be regulated by changing dietary amylose/amylopectin ratio (DAR) when weaned piglets under lipopolysaccharide (LPS) stress.
Methods: Sixty male weaned piglets (Duroc × Landrace × Yorkshire, 21 day old, 6.51 ± 0.64 kg) were allotted to 5 dietary treatments with 12 cages per treatment, and fed ad libitum with diets different in DAR (0.00, 0.20, 0.40, 0.60 and 0.80). Feed transformation happened at day 15. On day 29, 12 h before slaughter, pigs were intraperitoneal injected with 100 μg/kg body weight LPS or sterile saline.
Results: Results showed that LPS stress caused increase in serum urea nitrogen and glucose concentration (P < 0.05). Serum immunoglobulin G concentration increased after LPS stress in diet 0.80 but decreased in other groups (P<0.05). Serum serine concentrations decreased after LPS stress in diets 0.20 and 0.80 but increased in other groups (P<0.05). All tested hepatic amino acids (AA) increased after LPS stress (P<0.05) but hepatic isoleucine, leucine, threonine, aspartate, serine, glutamate, glycine concentrations decreased after LPS stress in diet 0.20 (P<0.05). Piglets supplemented diet with DAR 0.00 had highest superoxide dismutase (SOD1), nuclear factor 2 (Nrf2) and glutathione peroxidase 1 (GPX1) mRNA expression in liver (P<0.05). Total superoxide dismutase (T-SOD) activity of liver increased in diet 0.00 and 0.80 but decreased in diet 0.40 (P<0.05). Total antioxidant capacity (T-AOC) activity of longissimus dorsi muscle (LM) increased in diets 0.20 and 0.80 but decreased in diet 0.40 (P<0.05). Piglets in diet 0.20 had the highest hepatic glutathione peroxidase activity compared with groups 0.40, 0.60,0.80 (P<0.05). Mn concentration of LM and hepatic Cu decreased after LPS stress in diet 0.20 but increased in diet 0.80 (P<0.05). Piglets in diet 0.80 had the highest hepatic Cu, Fe, Mn, Zn concentrations (P<0.05). Cecal indol(e) concentration was higher in diet 0.00 than in diet 0.80 (P<0.05).
Conclusions: Antioxidant ability could be improved by feeding diet with low or high amylose/amylopectin ratio when weaned piglets suffered LPS stress.
Keywords
Dietary amylose/amylopectin ratio, LPS stress, amino acid, antioxidant activity, weaned piglet
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Research
Effect of Dietary Amylose/amylopectin Ratio on Antioxidant Ability and Amino Acid Metabolism of Liver in Weaned Pigs Challenged with Lipopolysaccharide
huansheng yang
Hunan Normal University; Hengyang Normal University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1164-5771
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 07 Jan, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Antioxidant function could be regulated by resistant starch supplementation in rats with kidney disease. It is controversial whether antioxidant ability could be regulated by changing dietary amylose/amylopectin ratio (DAR) when weaned piglets under lipopolysaccharide (LPS) stress.
Methods: Sixty male weaned piglets (Duroc × Landrace × Yorkshire, 21 day old, 6.51 ± 0.64 kg) were allotted to 5 dietary treatments with 12 cages per treatment, and fed ad libitum with diets different in DAR (0.00, 0.20, 0.40, 0.60 and 0.80). Feed transformation happened at day 15. On day 29, 12 h before slaughter, pigs were intraperitoneal injected with 100 μg/kg body weight LPS or sterile saline.
Results: Results showed that LPS stress caused increase in serum urea nitrogen and glucose concentration (P < 0.05). Serum immunoglobulin G concentration increased after LPS stress in diet 0.80 but decreased in other groups (P<0.05). Serum serine concentrations decreased after LPS stress in diets 0.20 and 0.80 but increased in other groups (P<0.05). All tested hepatic amino acids (AA) increased after LPS stress (P<0.05) but hepatic isoleucine, leucine, threonine, aspartate, serine, glutamate, glycine concentrations decreased after LPS stress in diet 0.20 (P<0.05). Piglets supplemented diet with DAR 0.00 had highest superoxide dismutase (SOD1), nuclear factor 2 (Nrf2) and glutathione peroxidase 1 (GPX1) mRNA expression in liver (P<0.05). Total superoxide dismutase (T-SOD) activity of liver increased in diet 0.00 and 0.80 but decreased in diet 0.40 (P<0.05). Total antioxidant capacity (T-AOC) activity of longissimus dorsi muscle (LM) increased in diets 0.20 and 0.80 but decreased in diet 0.40 (P<0.05). Piglets in diet 0.20 had the highest hepatic glutathione peroxidase activity compared with groups 0.40, 0.60,0.80 (P<0.05). Mn concentration of LM and hepatic Cu decreased after LPS stress in diet 0.20 but increased in diet 0.80 (P<0.05). Piglets in diet 0.80 had the highest hepatic Cu, Fe, Mn, Zn concentrations (P<0.05). Cecal indol(e) concentration was higher in diet 0.00 than in diet 0.80 (P<0.05).
Conclusions: Antioxidant ability could be improved by feeding diet with low or high amylose/amylopectin ratio when weaned piglets suffered LPS stress.