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Primary research
Zunfu Ke
Sun Yat-sen University First Affiliated Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6251-7246
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Replication factor C (RFC) proteins play a very important role in nuclear DNA replication, mismatch repair and cell cycle checkpoint pathways. However, the relationship between RFCs and brain tumors is still unclear, especially the diagnostic and prognostic significance of RFCs in low-grade glioma.
Methods: In our study, we applied the Oncomine, GEPIA, Human Protein Atlas, cBioPortal, STRING, LinkedOmics and Tumor Immune Estimation Resource (TIMER) databases to analyze the transcriptional and survival data of RFCs in brain and central nervous system (CNS) cancer, especially in low-grade glioma.
Results: We found that RFCs were highly expressed in brain and CNS cancer, including low-grade glioma. The transcriptional levels of RFCs were also associated with the tumor stages. Moreover, the survival analysis of RFCs in low-grade glioma patients revealed that enhanced expression of RFCs led to poorer prognosis. RFCs and their 50 frequently altered neighbor genes were found enriched in certain pathways. We also discovered the kinase targets, transcription factor targets and miRNA targets of RFCs in low-grade glioma. RFCs also showed positive correlation with certain infiltrated immune cells in low-grade glioma.
Conclusions: These implied that RFCs are the possible biomarkers of the diagnosis and prognosis of low-grade glioma.
Keywords
Replication factor C, low-grade glioma, bioinformatic analysis, RFC1, RFC2, RFC3, RFC4, RFC5
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This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.xlsx
File name: Additional file 1.xlxs Title of data: The GO enrichment analysis of RFCs and 50 most frequently altered neighboring genes in LGG.
- Additionalfile2.xlsx
File name: Additional file 2.xlxs Title of data: The enrichment analysis of different expressed RFCs and 50 most frequently altered neighboring genes in LGG.
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Primary research
Zunfu Ke
Sun Yat-sen University First Affiliated Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6251-7246
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 07 Jan, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Replication factor C (RFC) proteins play a very important role in nuclear DNA replication, mismatch repair and cell cycle checkpoint pathways. However, the relationship between RFCs and brain tumors is still unclear, especially the diagnostic and prognostic significance of RFCs in low-grade glioma.
Methods: In our study, we applied the Oncomine, GEPIA, Human Protein Atlas, cBioPortal, STRING, LinkedOmics and Tumor Immune Estimation Resource (TIMER) databases to analyze the transcriptional and survival data of RFCs in brain and central nervous system (CNS) cancer, especially in low-grade glioma.
Results: We found that RFCs were highly expressed in brain and CNS cancer, including low-grade glioma. The transcriptional levels of RFCs were also associated with the tumor stages. Moreover, the survival analysis of RFCs in low-grade glioma patients revealed that enhanced expression of RFCs led to poorer prognosis. RFCs and their 50 frequently altered neighbor genes were found enriched in certain pathways. We also discovered the kinase targets, transcription factor targets and miRNA targets of RFCs in low-grade glioma. RFCs also showed positive correlation with certain infiltrated immune cells in low-grade glioma.
Conclusions: These implied that RFCs are the possible biomarkers of the diagnosis and prognosis of low-grade glioma.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.xlsx
File name: Additional file 1.xlxs Title of data: The GO enrichment analysis of RFCs and 50 most frequently altered neighboring genes in LGG.
- Additionalfile2.xlsx
File name: Additional file 2.xlxs Title of data: The enrichment analysis of different expressed RFCs and 50 most frequently altered neighboring genes in LGG.
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