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LncRNA FBXL19-AS1 Promotes Proliferation and Metastasis of Cervical Cancer Through Upregulating COL1A1 as a Sponge of MiR-193a-5p
Xuemei Jiang
Xinjiang Uygur Autonomous Region Maternal and Child Health Hospital
Corresponding Author
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Background: Cervical cancer (CC) is one of the most common and malignant tumors in women. In this study, we aim to explore the role and mechanism of FBXL19-AS1, a novel long-chain non coding RNA with marked roles in a variety of tumors, in regulating the proliferation and metastasis of CC. The expression of FBXL19-AS1, miR-193a-5p and COL1A1 were detected by RT-PCR and western blot. Gain-and loss- of functions of FBXL19-AS1 and miR-193a-5p were performed in CC cell lines in vitro. The proliferation, migration, invasion, apoptosis and epithelial-mesenchymal transition (EMT) level were determined.
Results: FBXL19-AS1 and COL1A1 were significantly up-regulated in CC, while miR-193a-5p was significantly down-regulated. Overexpression of FBXL19-AS1 significantly promoted the proliferation, migration, invasion and EMT of CC cells and inhibited apoptosis, while knockdown of FBXL19-AS1 had the opposite effects. On the other hand, miR-193a-5p inhibited the proliferation and metastasis of CC cells. Mechanistically, FBXL19-AS1 functioned as a competitive endogenous RNA and inhibited the expression of miR-193a-5p, which targeted at the 3'-UTR site of COL1A1 and negatively regulated COL1A1 expression.
Concluson: FBXL19-AS1 can promote the proliferation and metastasis of CC by sponging miR-193a-5p and up-regulating COL1A1.
Keywords
Cervical cancer, proliferation, metastasis, FBXL19-AS1, miR-193a-5p, COL1A1
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CURRENT STATUS: Under Review
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Posted 07 Jan, 2021
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Invitations sent on 04 Jan, 2021
Reviewer #1 agreed
On 04 Jan, 2021
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This preprint is under consideration at Journal of Biological Research-Thessaloniki. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
LncRNA FBXL19-AS1 Promotes Proliferation and Metastasis of Cervical Cancer Through Upregulating COL1A1 as a Sponge of MiR-193a-5p
Xuemei Jiang
Xinjiang Uygur Autonomous Region Maternal and Child Health Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 07 Jan, 2021
No community comments so far
Reviewers invited
Invitations sent on 04 Jan, 2021
Reviewer #1 agreed
On 04 Jan, 2021
First submitted
On 30 Dec, 2020
Editor assigned
On 30 Dec, 2020
Submission checks complete
On 30 Dec, 2020
Editor invited
On 30 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Cervical cancer (CC) is one of the most common and malignant tumors in women. In this study, we aim to explore the role and mechanism of FBXL19-AS1, a novel long-chain non coding RNA with marked roles in a variety of tumors, in regulating the proliferation and metastasis of CC. The expression of FBXL19-AS1, miR-193a-5p and COL1A1 were detected by RT-PCR and western blot. Gain-and loss- of functions of FBXL19-AS1 and miR-193a-5p were performed in CC cell lines in vitro. The proliferation, migration, invasion, apoptosis and epithelial-mesenchymal transition (EMT) level were determined.
Results: FBXL19-AS1 and COL1A1 were significantly up-regulated in CC, while miR-193a-5p was significantly down-regulated. Overexpression of FBXL19-AS1 significantly promoted the proliferation, migration, invasion and EMT of CC cells and inhibited apoptosis, while knockdown of FBXL19-AS1 had the opposite effects. On the other hand, miR-193a-5p inhibited the proliferation and metastasis of CC cells. Mechanistically, FBXL19-AS1 functioned as a competitive endogenous RNA and inhibited the expression of miR-193a-5p, which targeted at the 3'-UTR site of COL1A1 and negatively regulated COL1A1 expression.
Concluson: FBXL19-AS1 can promote the proliferation and metastasis of CC by sponging miR-193a-5p and up-regulating COL1A1.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7