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Bohua Chen
affiliated hospital of qiingdao university
Corresponding Author
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Our previous study revealed that epidural adipose-derived stem cells (ADSCs) respond to lung cancer cells and are involved in forming premetastatic niches. The biological functions of epidural ADSCs in lymphoma are still unclear. In this study, we first confirmed the B cell lymphoma-promoting effects of epidural ADSC exosomes and then compared differentially expressed genes in lymphoma cells before and after exosome treatment. In ADSC exosome-treated lymphoma cells, S100A4 expression was much higher than that in cells cultured alone. S100A4 levels had a direct relationship with EMT in cells and were also associated with the survival of lymphoma patients. Then, we tested the roles of ADSC exosomes and S100A4 in the lymphoma-promoting impacts of ADSCs. Our results showed that ADSC exosomes could foster the proliferation, EMT and invasion of lymphoma cells, as could the increase in S100A4 expression. S100A4 inhibition in two lymphoma cell lines suppressed the lymphoma cell proliferation’s ADSC exosome-mediated promotion, invasion and EMT in vitro. Moreover, S100A4 inhibition attenuated metastasis and tumour growth, and ADSC exosomes promoted tumour progression, as demonstrated in a nude mouse model of lymphoma. On the basis of these data, ADSC exosomes foster B cell lymphoma progression by increasing S100A4 expression in lymphoma cells.
Keywords
Exosomes Secreted, Epidural Adipose, Lymphoma
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Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- Fig.S1.tif
Isolation and identification of ADSC exosomes, and internalization of exosomes by lymphoma cell. (A): ADSC exosomes were visualized by electron microscopy (×30,000). (B): Western blot examined the expression of exosomes marker TSG101 and CD63 in isolated ADSC exosomes. (C): Typical imagines of internalizated exosomes derived from epidural ADSCs by B cell lymphoma cell line SU-DHL-10 at 4 h. Fluorescence microscopy images showing the internalization of exosomes by SU-DHL-10 cells. Blue: Nucleus stained with DAPI. Red: PKH26-labeled exosomes. Scale bar: 50 μm.
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Bohua Chen
affiliated hospital of qiingdao university
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 08 Jan, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Cell Biology & Physiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Our previous study revealed that epidural adipose-derived stem cells (ADSCs) respond to lung cancer cells and are involved in forming premetastatic niches. The biological functions of epidural ADSCs in lymphoma are still unclear. In this study, we first confirmed the B cell lymphoma-promoting effects of epidural ADSC exosomes and then compared differentially expressed genes in lymphoma cells before and after exosome treatment. In ADSC exosome-treated lymphoma cells, S100A4 expression was much higher than that in cells cultured alone. S100A4 levels had a direct relationship with EMT in cells and were also associated with the survival of lymphoma patients. Then, we tested the roles of ADSC exosomes and S100A4 in the lymphoma-promoting impacts of ADSCs. Our results showed that ADSC exosomes could foster the proliferation, EMT and invasion of lymphoma cells, as could the increase in S100A4 expression. S100A4 inhibition in two lymphoma cell lines suppressed the lymphoma cell proliferation’s ADSC exosome-mediated promotion, invasion and EMT in vitro. Moreover, S100A4 inhibition attenuated metastasis and tumour growth, and ADSC exosomes promoted tumour progression, as demonstrated in a nude mouse model of lymphoma. On the basis of these data, ADSC exosomes foster B cell lymphoma progression by increasing S100A4 expression in lymphoma cells.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- Fig.S1.tif
Isolation and identification of ADSC exosomes, and internalization of exosomes by lymphoma cell. (A): ADSC exosomes were visualized by electron microscopy (×30,000). (B): Western blot examined the expression of exosomes marker TSG101 and CD63 in isolated ADSC exosomes. (C): Typical imagines of internalizated exosomes derived from epidural ADSCs by B cell lymphoma cell line SU-DHL-10 at 4 h. Fluorescence microscopy images showing the internalization of exosomes by SU-DHL-10 cells. Blue: Nucleus stained with DAPI. Red: PKH26-labeled exosomes. Scale bar: 50 μm.
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