DOI: https://doi.org/10.21203/rs.2.23602/v1
Background: Kawasaki disease (KD) is an acute and systemic vasculitis, and the critical complication in KD patients is coronary artery lesions (CAL). Plasma miR-181a was found dysregulated in a variety of cardiovascular disease. The aim of this study was to define the relationship between the plasma miR-181a levels and CAL in KD. Methods: Plasma miR-181a levels were analyzed by quantitative reverse transcriptase-polymerase chain reaction in 121 patients with KD. Results:We found that plasma miR-181a levels at the acute phase were significantly elevated in KD patients with CAL than those without CAL. Correlation analysis showed that plasma miR-181a levels were positively correlated with the concentrations of CRP (r=0.363, P < 0.05) and NT-proBNP (r=0.389, P < 0.05). Receiver operating characteristic curve analyses showed that plasma miR-181a was of significant prediction value for CAL in KD, the area under receiver operating characteristic curve value for plasma miR-181a in prediction of CAL was 0.747, and the estimated sensitivity and specificity were 75.0% and 68.8%, respectively. Conclusions: Plasma miR-181a is prone to be a candidate biomarker for predicting CAL in KD. Therefore, further investigations are warranted to fully elucidate its role in KD.
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Table 1 Demographic, clinical and laboratory characteristics of patients with KD
|
Table 2 Comparison of laboratory characteristic in KD patients with and without CAL
|
KD with CAL |
KD without CAL |
P value |
Age (months) |
18 (2,120) |
19 (2,144) |
0.354 |
Male |
18 (69.2%) |
56 (58.9%) |
0.340 |
WBC (×109/l) |
17.85±6.87 |
15.54±5.35 |
0.082 |
Neutrophil (×109/l) |
12.70±5.77 |
10.43±4.49 |
0.043* |
Lymphocyte (×109/l) |
3.59±2.10 |
3.61±2.14 |
0.961 |
Hemoglobin (g/l) |
101.56±12.80 |
103.59±10.98 |
0.362 |
Platelet (×109/l) |
372.53±152.43 |
350.56±107.05 |
0.444 |
CRP (mg/dl) |
118.83±65.46 |
99.06±60.52 |
0.012* |
ESR (mm/h) |
64.84±23.82 |
60.27±27.51 |
0.368 |
PCT (ng/ml) |
1.44±1.77 |
2.25±13.04 |
0.530 |
NT-proBNP (pg/ml) |
1393.35±1792.47 |
636.57±771.91 |
0.025* |
SF (ng/ml) |
288.84±311.36 |
279.70±198.82 |
0.311 |
Table 3 Correlation between circulating miR-181a levels and other laboratory data in KD patients
|
Correlation coefficient |
P value |
WBC |
0.019 |
0.807 |
Neutrophil |
-0.016 |
0.838 |
Lymphocyte |
0.086 |
0.263 |
Hemoglobin |
-0.039 |
0.609 |
Platelet |
-0.006 |
0.933 |
CRP |
0.363 |
<0.001 |
ESR |
0.041 |
0.595 |
PCT |
-0.058 |
0.451 |
NT-proBNP |
0.389 |
<0.001 |
SF |
0.132 |
0.084 |