Clinical, electrocardiographic and echocardiographic parameters in Pega donkeys are scarce in literature, hence this study was performed to evaluate the effect of sex and age on echocardiographic and electrocardiographic measurements in the Pega breed donkeys. The objectives of this study were to describe and illustrate the clinical, electrocardiographic and echocardiographic parameters in Pega donkeys. Fifty Pega breed donkeys were evaluated, with an average age of 3.4 years, 20 males and 30 females. Based on their age (younger or older than 6 years), animals were separated into two groups. In each animal the electrocardiographic examination was performed using the TEB® computerized system and the echocardiographic examination was performed using an ultrasound device with a doppler function multifrequency sectorial transducer, in 2D mode. Male echocardiographic measurements were significantly higher than females, except the velocity and pressure gradient of the pulmonary flow, where females had higher values than males. For the electrocardiographic parameters, females presented values of P wave amplitude, R wave amplitude, S wave amplitude and T wave amplitude higher than those found in males. Males had higher values for P wave duration, QRS complex and RR interval. Standardizing the electrocardiographic and echocardiographic parameters for the Pega breed donkey can contribute to future assessments regarding possible changes that excessive effort can promote in these parameters, to a management engrossed on animal welfare. Furthermore, as well as in other species, gender in donkeys should be considered as a factor that influences echocardiographic and electrocardiographic parameters.