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Research article
Jinfeng Yang
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8760-2244
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: Several investigations have indicated that T helper cell 17 (Th 17 ) and Foxp 3 + regulatory T cells (Treg) cells adopt a pro-tumorigenic role. Opioids and pain exacerbate immunosuppression in immunocompromised cancer patients. Methods: A rat hepatocellular carcinoma (HCC) models was established by N-nitrosodiethylamine (DENA). 48 of them were randomly divided into 3 groups (n=16): surgery without postoperative analgesia (Control); surgery with morphine postoperative analgesia (Morphine); surgery with sufentanil postoperative analgesia (Sufentanil). The level of cluster of CD4 + , CD8 + , Th1,Th2, Th17 and Treg cells in blood were also detected to assess immune function using flow cytometry on d0, d3 and d7.The rats’ survival situation of each group left after three days of surgery were observed. Results ： The CD4 + /CD8 + ratio and Th1 cells levels were significantly higher while Th2, Th17 and Treg cells levels were significantly lower in sufentanil and morphine postoperative analgesia rats compared with that without postoperative analgesia. Compared with morphine postoperative analgesia, sufentanil postoperative analgesia rats seem have higher CD4+/CD8+ ratio , Th1 cells, while lower Th2, Th17 and Treg cells level. Conclusions ： Sufentanil and morphine postoperative analgesia show better immune function than that without postoperative analgesia by analyzing CD4 + /CD8 + ratio, Th1, Th2, Th17 and Treg cells. Sufentanil postoperative analgesia demonstrated a favorable impact on immune function compared with morphine postoperative anesthesia in hepatocellular carcinoma rats undergoing left hepatectomy operation.
Keywords
Sufentanil； analgesia； Th17； Treg
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View the published article at BMC Anesthesiology.
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Posted 14 Feb, 2020
No community comments so far
Editorial decision: Major revision
On 26 Mar, 2020
Review #2 received
Received 25 Mar, 2020
Reviewer #2 agreed
On 09 Mar, 2020
Review #1 received
Received 06 Mar, 2020
Reviewer #1 agreed
On 21 Feb, 2020
Reviewers invited
Invitations sent on 19 Feb, 2020
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On 11 Feb, 2020
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On 10 Feb, 2020
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Subject Areas
Anesthesiology & Pain Medicine
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A new preprint design is coming!
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See the published version of this preprint at BMC Anesthesiology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Jinfeng Yang
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8760-2244
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Anesthesiology.
Version 1
Posted 14 Feb, 2020
No community comments so far
Editorial decision: Major revision
On 26 Mar, 2020
Review #2 received
Received 25 Mar, 2020
Reviewer #2 agreed
On 09 Mar, 2020
Review #1 received
Received 06 Mar, 2020
Reviewer #1 agreed
On 21 Feb, 2020
Reviewers invited
Invitations sent on 19 Feb, 2020
Editor assigned
On 11 Feb, 2020
Submission checks complete
On 10 Feb, 2020
Editor invited
On 10 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Anesthesiology & Pain Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Anesthesiology.
Background: Several investigations have indicated that T helper cell 17 (Th 17 ) and Foxp 3 + regulatory T cells (Treg) cells adopt a pro-tumorigenic role. Opioids and pain exacerbate immunosuppression in immunocompromised cancer patients. Methods: A rat hepatocellular carcinoma (HCC) models was established by N-nitrosodiethylamine (DENA). 48 of them were randomly divided into 3 groups (n=16): surgery without postoperative analgesia (Control); surgery with morphine postoperative analgesia (Morphine); surgery with sufentanil postoperative analgesia (Sufentanil). The level of cluster of CD4 + , CD8 + , Th1,Th2, Th17 and Treg cells in blood were also detected to assess immune function using flow cytometry on d0, d3 and d7.The rats’ survival situation of each group left after three days of surgery were observed. Results ： The CD4 + /CD8 + ratio and Th1 cells levels were significantly higher while Th2, Th17 and Treg cells levels were significantly lower in sufentanil and morphine postoperative analgesia rats compared with that without postoperative analgesia. Compared with morphine postoperative analgesia, sufentanil postoperative analgesia rats seem have higher CD4+/CD8+ ratio , Th1 cells, while lower Th2, Th17 and Treg cells level. Conclusions ： Sufentanil and morphine postoperative analgesia show better immune function than that without postoperative analgesia by analyzing CD4 + /CD8 + ratio, Th1, Th2, Th17 and Treg cells. Sufentanil postoperative analgesia demonstrated a favorable impact on immune function compared with morphine postoperative anesthesia in hepatocellular carcinoma rats undergoing left hepatectomy operation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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