Comparative iTRAQ Proteomics Identified Proteins Associated With Sperm Maturation Between Yak and Cattleyak Epididymis
Background: During maturation, spermatozoa acquire motility and fertilizing capacity as they transit through the epididymis. In recent years, two-dimensional gel electrophoresis has been employed in proteomics studies conducted in rat, boar and human. However, there has not been a complete information regarding the proteins associated with sperm maturation in the epididymis. In this study, we employed iTRAQ proteomics to investigate proteins associated with sperm maturation between yak and cattleyak epididymis.
Results: After successfully establishing the samples and protein extraction, the iTRAQ coupled with LC-MS/MS mass spectrometry and bioinformatics analysis was performed. We identified 288 DAPs between yak and cattleyak epididymis; 151 were up-regulated while 137 were down-regulated in cattleyak relative to yak. Gene Ontology analysis identified that down-regulated DAPs in cattleyak were mostly enriched in the acetylation of protein component, negative regulatory activity, and positive regulatory activity. iTRAQ proteomics data showed that the top down-regulated DAPs were predominantly associated with sperm maturation, long-term sperm storage, sperm forward motility, sperm-oocyte fusion, and regulatory functions, while, the top up-regulated DAPs were mainly enriched in cell communication, cell adhesion, cytoskeleton organization, stress response, post-translational modifications and metabolic functions.
Conclusion: These results provide insight into the molecular mechanisms underlying male cattleyak infertility.
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Posted 12 Jan, 2021
Received 05 Feb, 2021
On 05 Feb, 2021
Received 31 Jan, 2021
On 12 Jan, 2021
On 08 Jan, 2021
On 07 Jan, 2021
Invitations sent on 07 Jan, 2021
On 06 Jan, 2021
On 04 Jan, 2021
On 11 Nov, 2020
Comparative iTRAQ Proteomics Identified Proteins Associated With Sperm Maturation Between Yak and Cattleyak Epididymis
Posted 12 Jan, 2021
Received 05 Feb, 2021
On 05 Feb, 2021
Received 31 Jan, 2021
On 12 Jan, 2021
On 08 Jan, 2021
On 07 Jan, 2021
Invitations sent on 07 Jan, 2021
On 06 Jan, 2021
On 04 Jan, 2021
On 11 Nov, 2020
Background: During maturation, spermatozoa acquire motility and fertilizing capacity as they transit through the epididymis. In recent years, two-dimensional gel electrophoresis has been employed in proteomics studies conducted in rat, boar and human. However, there has not been a complete information regarding the proteins associated with sperm maturation in the epididymis. In this study, we employed iTRAQ proteomics to investigate proteins associated with sperm maturation between yak and cattleyak epididymis.
Results: After successfully establishing the samples and protein extraction, the iTRAQ coupled with LC-MS/MS mass spectrometry and bioinformatics analysis was performed. We identified 288 DAPs between yak and cattleyak epididymis; 151 were up-regulated while 137 were down-regulated in cattleyak relative to yak. Gene Ontology analysis identified that down-regulated DAPs in cattleyak were mostly enriched in the acetylation of protein component, negative regulatory activity, and positive regulatory activity. iTRAQ proteomics data showed that the top down-regulated DAPs were predominantly associated with sperm maturation, long-term sperm storage, sperm forward motility, sperm-oocyte fusion, and regulatory functions, while, the top up-regulated DAPs were mainly enriched in cell communication, cell adhesion, cytoskeleton organization, stress response, post-translational modifications and metabolic functions.
Conclusion: These results provide insight into the molecular mechanisms underlying male cattleyak infertility.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7