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Research article
Comparative iTRAQ Proteomics Identified Proteins Associated With Sperm Maturation Between Yak and Cattleyak Epididymis
Xin Cai
Southwest Minzu University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: During maturation, spermatozoa acquire motility and fertilizing capacity as they transit through the epididymis. In recent years, two-dimensional gel electrophoresis has been employed in proteomics studies conducted in rat, boar and human. However, there has not been a complete information regarding the proteins associated with sperm maturation in the epididymis. In this study, we employed iTRAQ proteomics to investigate proteins associated with sperm maturation between yak and cattleyak epididymis.
Results: After successfully establishing the samples and protein extraction, the iTRAQ coupled with LC-MS/MS mass spectrometry and bioinformatics analysis was performed. We identified 288 DAPs between yak and cattleyak epididymis; 151 were up-regulated while 137 were down-regulated in cattleyak relative to yak. Gene Ontology analysis identified that down-regulated DAPs in cattleyak were mostly enriched in the acetylation of protein component, negative regulatory activity, and positive regulatory activity. iTRAQ proteomics data showed that the top down-regulated DAPs were predominantly associated with sperm maturation, long-term sperm storage, sperm forward motility, sperm-oocyte fusion, and regulatory functions, while, the top up-regulated DAPs were mainly enriched in cell communication, cell adhesion, cytoskeleton organization, stress response, post-translational modifications and metabolic functions.
Conclusion: These results provide insight into the molecular mechanisms underlying male cattleyak infertility.
Keywords
Yak, Cattleyak, Epididymis, Sperm maturation, iTRAQ proteomics
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CURRENT STATUS: Under Review
Version 1
Posted 12 Jan, 2021
No community comments so far
Review #2 received
Received 05 Feb, 2021
Editorial decision: Major revision
On 05 Feb, 2021
Review #1 received
Received 31 Jan, 2021
Reviewer #2 agreed
On 12 Jan, 2021
Reviewer #1 agreed
On 08 Jan, 2021
Editor assigned
On 07 Jan, 2021
Reviewers invited
Invitations sent on 07 Jan, 2021
Submission checks complete
On 06 Jan, 2021
Editor invited
On 04 Jan, 2021
First submitted
On 11 Nov, 2020
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This preprint is under consideration at BMC Veterinary Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Comparative iTRAQ Proteomics Identified Proteins Associated With Sperm Maturation Between Yak and Cattleyak Epididymis
Xin Cai
Southwest Minzu University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 12 Jan, 2021
No community comments so far
Review #2 received
Received 05 Feb, 2021
Editorial decision: Major revision
On 05 Feb, 2021
Review #1 received
Received 31 Jan, 2021
Reviewer #2 agreed
On 12 Jan, 2021
Reviewer #1 agreed
On 08 Jan, 2021
Editor assigned
On 07 Jan, 2021
Reviewers invited
Invitations sent on 07 Jan, 2021
Submission checks complete
On 06 Jan, 2021
Editor invited
On 04 Jan, 2021
First submitted
On 11 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: During maturation, spermatozoa acquire motility and fertilizing capacity as they transit through the epididymis. In recent years, two-dimensional gel electrophoresis has been employed in proteomics studies conducted in rat, boar and human. However, there has not been a complete information regarding the proteins associated with sperm maturation in the epididymis. In this study, we employed iTRAQ proteomics to investigate proteins associated with sperm maturation between yak and cattleyak epididymis.
Results: After successfully establishing the samples and protein extraction, the iTRAQ coupled with LC-MS/MS mass spectrometry and bioinformatics analysis was performed. We identified 288 DAPs between yak and cattleyak epididymis; 151 were up-regulated while 137 were down-regulated in cattleyak relative to yak. Gene Ontology analysis identified that down-regulated DAPs in cattleyak were mostly enriched in the acetylation of protein component, negative regulatory activity, and positive regulatory activity. iTRAQ proteomics data showed that the top down-regulated DAPs were predominantly associated with sperm maturation, long-term sperm storage, sperm forward motility, sperm-oocyte fusion, and regulatory functions, while, the top up-regulated DAPs were mainly enriched in cell communication, cell adhesion, cytoskeleton organization, stress response, post-translational modifications and metabolic functions.
Conclusion: These results provide insight into the molecular mechanisms underlying male cattleyak infertility.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7