Utilizing cellulose nanofibers (CNF) attracts tremendous attention in terms of developing a non-breakable film with high toughness and optically transparent properties. Here, the author introduces a new characterization method in order to compare the relative degree of the crystalline and amorphous characters of CNF by correlating Graphene-Quenching Raman Scattering (GQRS) with Electrostatic Force Microscope (EFM) results. This nano-metrology may pave the new way in quantitatively unraveling the degree of lignin and hemicellulose.