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Maria Vikentjeva
Tervise Arengu Instituut
Corresponding Author
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BACKGROUND: Rickettsia spp. are human pathogens that cause a number of diseases and are transmitted by arthropods, such as ixodid ticks. Estonia is one of few regions where the distribution area of two medically important tick species, Ixodes persulcatus and I. ricinus, overlaps. The presence of the nidicolous rodent-associated I. trianguliceps has also recently been shown in Estonia. Although there is no data available in Estonia on human disease caused by tick-borne Rickettsia spp., the presence of three Rickettsia species in non-nidicolous ticks was also previously reported. The aim of this study was to detect, identify and partially characterize Rickettsia species in nidicolous and non-nidicolous ticks attached to rodents.
RESULTS: Larvae and nymphs of I. ricinus (n = 1004), I. persulcatus (n = 75) and I. trianguliceps (n = 117) removed from rodents and shrews caught in different parts of Estonia were studied for the presence of Rickettsia spp. by nested PCR. Ticks were collected from 314 small animals of 5 species (Myodes glareolus (bank voles), Apodemus flavicollis (yellow necked mice), A. agrarius (striped field mice), Microtus subterranius (pine voles) and Sorex araneus (common shrews)). Rickettsial DNA was detected in 8.7% (103/1186) of the studied ticks. In addition to R. helvetica previously found in questing ticks, this study reports the first identification of the recently described I. trianguliceps-associated Candidatus R. uralica west of the Ural Mountains.
Keywords
Ticks, Rickettsia spp., Candidatus Rickettsia uralica, voles, mice
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CURRENT STATUS: Published
View the published article at Parasites & Vectors.
Version 4
Posted 06 Jan, 2021
No community comments so far
Editorial decision: Accept
On 25 Dec, 2020
Editor invited
On 22 Dec, 2020
Editor assigned
On 22 Dec, 2020
Submission checks complete
On 22 Dec, 2020
No comments provided
Editorial decision: Major Revision
On 21 Nov, 2020
Review #1 received
Received 27 Apr, 2020
Review #2 received
Received 27 Apr, 2020
Reviewer #2 agreed
On 20 Apr, 2020
Reviewers invited
Invitations sent on 16 Apr, 2020
Reviewer #1 agreed
On 16 Apr, 2020
Editor assigned
On 25 Mar, 2020
Submission checks complete
On 24 Mar, 2020
Editor invited
On 24 Mar, 2020
No comments provided
Editorial decision: Revise before peer review
On 09 Mar, 2020
Editor assigned
On 28 Feb, 2020
Submission checks complete
On 27 Feb, 2020
Editor invited
On 27 Feb, 2020
No comments provided
Editorial decision: Revise before peer review
On 22 Feb, 2020
Editor invited
On 18 Feb, 2020
Editor assigned
On 18 Feb, 2020
First submitted
On 17 Feb, 2020
Submission checks complete
On 17 Feb, 2020
Metrics
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PDF Downloads: ...
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Subject Areas
Parasitology
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A new preprint design is coming!
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See the published version of this preprint at Parasites & Vectors.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Maria Vikentjeva
Tervise Arengu Instituut
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Parasites & Vectors.
Version 4
Posted 06 Jan, 2021
No community comments so far
Editorial decision: Accept
On 25 Dec, 2020
Editor invited
On 22 Dec, 2020
Editor assigned
On 22 Dec, 2020
Submission checks complete
On 22 Dec, 2020
No comments provided
Editorial decision: Major Revision
On 21 Nov, 2020
Review #1 received
Received 27 Apr, 2020
Review #2 received
Received 27 Apr, 2020
Reviewer #2 agreed
On 20 Apr, 2020
Reviewers invited
Invitations sent on 16 Apr, 2020
Reviewer #1 agreed
On 16 Apr, 2020
Editor assigned
On 25 Mar, 2020
Submission checks complete
On 24 Mar, 2020
Editor invited
On 24 Mar, 2020
No comments provided
Editorial decision: Revise before peer review
On 09 Mar, 2020
Editor assigned
On 28 Feb, 2020
Submission checks complete
On 27 Feb, 2020
Editor invited
On 27 Feb, 2020
No comments provided
Editorial decision: Revise before peer review
On 22 Feb, 2020
Editor invited
On 18 Feb, 2020
Editor assigned
On 18 Feb, 2020
First submitted
On 17 Feb, 2020
Submission checks complete
On 17 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Parasites & Vectors.
BACKGROUND: Rickettsia spp. are human pathogens that cause a number of diseases and are transmitted by arthropods, such as ixodid ticks. Estonia is one of few regions where the distribution area of two medically important tick species, Ixodes persulcatus and I. ricinus, overlaps. The presence of the nidicolous rodent-associated I. trianguliceps has also recently been shown in Estonia. Although there is no data available in Estonia on human disease caused by tick-borne Rickettsia spp., the presence of three Rickettsia species in non-nidicolous ticks was also previously reported. The aim of this study was to detect, identify and partially characterize Rickettsia species in nidicolous and non-nidicolous ticks attached to rodents.
RESULTS: Larvae and nymphs of I. ricinus (n = 1004), I. persulcatus (n = 75) and I. trianguliceps (n = 117) removed from rodents and shrews caught in different parts of Estonia were studied for the presence of Rickettsia spp. by nested PCR. Ticks were collected from 314 small animals of 5 species (Myodes glareolus (bank voles), Apodemus flavicollis (yellow necked mice), A. agrarius (striped field mice), Microtus subterranius (pine voles) and Sorex araneus (common shrews)). Rickettsial DNA was detected in 8.7% (103/1186) of the studied ticks. In addition to R. helvetica previously found in questing ticks, this study reports the first identification of the recently described I. trianguliceps-associated Candidatus R. uralica west of the Ural Mountains.
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
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