Background. Long noncoding RNAs (lncRNAs) perform as competing endogenous RNAs (ceRNAs) to sponge microRNAs (miRNAs) lead to the advancement of cancer. In this work, we attempted to identify possible long noncoding RNA biomarkers as well as a new regulatory axis in colorectal cancer. This investigation was carried out in consideration of the critical oncogenic function played by miR-616-3p in many malignancies, as well as its regulatory involvement in several signaling pathways.
Material and Methods: Differentially expressed lncRNAs (DELs) were identified from Gene Expression Omnibus (GEO) database. Targeted mRNAs were recognized by the Targetscan database. ciBioPortal database and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also were applied to identify mRNAs. CeRNA networks constructed via Cytoscape 3.7.1. QRT-PCR was utilized to verify these RNA molecules' expression levels in 30 CRC tissues compared to 30 adjacent tissue samples.
Results: Three lncRNAs and three mRNAs were discovered to have the greatest interaction with miR-616-3p. QRT-PCR revealed overexpression of (Linc01282, lnc-MYADM-1:1, ZNF347, XIAP) and downregulation of (Lnc-atp12a-1:1, SIK1 and miR-616-3p). In CRC, bioinformatic research revealed many unique ceRNA networks centered on miR-616-3p that were previously unknown.
Conclusion: This work reveals novel, previously unreported lncRNAs as prognostic biomarkers for CRC, as well as potential mRNAs as new therapeutic targets and predictive biomarkers for CRC. Furthermore, our analysis identified novel ceRNA networks that need be investigated further in CRC.