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Research article
Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance
Wenxiong Lin
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0930-5145
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Rice is a chilling-sensitive crop that would suffer serious damage from low temperatures. Overexpression of the Lsi1 gene (Lsi1-OX) in rice enhances its chilling tolerance. This study revealed that a serine hydroxymethyltransferase (OsSHMT) mainly localised in the endoplasmic reticulum (ER) is involved in increasing tolerance to chilling.
Results: A higher transcription level of OsSHMT was detected in Lsi1-OX rice than in the wild type. Histone H1 and nucleic acid binding protein were found to bind to the promoter region of OsSHMT and regulate its expression, and the transcription levels of these proteins were also up-regulated in the Lsi1-OX rice. Moreover, OsSHMT interacts with ATP synthase subunit α, heat shock protein Hsp70, mitochondrial substrate carrier family protein, ascorbate peroxidase 1 and ATP synthase subunit β. Lsi1-encoded protein OsNIP2;1 also interacts with ATP synthase subunit β, and the coordination of these proteins appears to function in reducing reactive oxygen species, as the H2O2 content of transgenic OsSHMT Arabidopsis thaliana was lower than that of the non-transgenic line under chilling treatment.
Conclusions: Our results indicate that ER-localised OsSHMT plays a role in scavenging H2O2 to enhance the chilling tolerance of Lsi1-OX rice and that ATP synthase subunit β is an intermediate junction between OsNIP2;1 and OsSHMT.
Keywords
Rice, chilling, serine hydroxymethyltransferase, ROS, protein-protein interactions
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Received 22 Apr, 2020
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On 31 Mar, 2020
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Received 28 Mar, 2020
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On 28 Mar, 2020
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Editorial decision: Major revision
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Received 12 Mar, 2020
Review #1 received
Received 05 Mar, 2020
Reviewer #2 agreed
On 28 Feb, 2020
Editor assigned
On 27 Feb, 2020
Reviewers invited
Invitations sent on 27 Feb, 2020
Reviewer #1 agreed
On 27 Feb, 2020
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance
Wenxiong Lin
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0930-5145
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 3
Posted 19 May, 2020
No community comments so far
Editor assigned
On 06 May, 2020
Editor invited
On 05 May, 2020
Submission checks complete
On 18 Feb, 2020
No comments provided
Editorial decision: Minor revision
On 05 May, 2020
Review #2 received
Received 22 Apr, 2020
Reviewer #2 agreed
On 31 Mar, 2020
Review #1 received
Received 28 Mar, 2020
Reviewers invited
Invitations sent on 28 Mar, 2020
Reviewer #1 agreed
On 28 Mar, 2020
Editor assigned
On 25 Mar, 2020
Submission checks complete
On 24 Mar, 2020
Editor invited
On 24 Mar, 2020
No comments provided
Editorial decision: Major revision
On 13 Mar, 2020
Review #2 received
Received 12 Mar, 2020
Review #1 received
Received 05 Mar, 2020
Reviewer #2 agreed
On 28 Feb, 2020
Editor assigned
On 27 Feb, 2020
Reviewers invited
Invitations sent on 27 Feb, 2020
Reviewer #1 agreed
On 27 Feb, 2020
Submission checks complete
On 19 Feb, 2020
Editor invited
On 19 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Rice is a chilling-sensitive crop that would suffer serious damage from low temperatures. Overexpression of the Lsi1 gene (Lsi1-OX) in rice enhances its chilling tolerance. This study revealed that a serine hydroxymethyltransferase (OsSHMT) mainly localised in the endoplasmic reticulum (ER) is involved in increasing tolerance to chilling.
Results: A higher transcription level of OsSHMT was detected in Lsi1-OX rice than in the wild type. Histone H1 and nucleic acid binding protein were found to bind to the promoter region of OsSHMT and regulate its expression, and the transcription levels of these proteins were also up-regulated in the Lsi1-OX rice. Moreover, OsSHMT interacts with ATP synthase subunit α, heat shock protein Hsp70, mitochondrial substrate carrier family protein, ascorbate peroxidase 1 and ATP synthase subunit β. Lsi1-encoded protein OsNIP2;1 also interacts with ATP synthase subunit β, and the coordination of these proteins appears to function in reducing reactive oxygen species, as the H2O2 content of transgenic OsSHMT Arabidopsis thaliana was lower than that of the non-transgenic line under chilling treatment.
Conclusions: Our results indicate that ER-localised OsSHMT plays a role in scavenging H2O2 to enhance the chilling tolerance of Lsi1-OX rice and that ATP synthase subunit β is an intermediate junction between OsNIP2;1 and OsSHMT.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8