A study of the phytochemicals of stems, Millettia phuwuaensis. This led to the discovery of solvent extracts and pure substances from chromatography. Compound (1), 7-methoxy-5/,6/-methylenedioxyisoflavone, isoflavonoid, and compound (2), 12-deoxo-12α-hydroxyelliptone, rotenoid were structurally proven using NMR-based spectroscopy. Both extracts and pure compounds were evaluated for their biological activity, including antibacterial activity, AIDs, and cancer. The antibacterial test results of the extracts and pure compound were found to be valuable, MIC is in the range of 12.5-200 mg/mL, in the range of 0.188-6 mg/mL, respectively. Mechanistic anti-AIDs affect RT and MC99 found that ethyl acetate extract inhibited the very high level with IC50 with 75.93 %. It was also found that all extracts were effective in inhibiting AIDs By mechanism MC99 at EC50 at 1.35 µM (TI>2.41). Further, the ethyl acetate extract showed marked cytotoxicity (ED50= 17.58 µg/ml against the SH-SY5Y cancer cell line. Additionally, compound 1 was also exhibited RT, moderately active with IC50 55.19 % inhibition. More than that, compounds 1 and 2 were also exhibited to MC99 at 50% (EC50) values of > 3.01 (TI >1.70) and 1.78 (TI >1.70), respectively.