Antiobesity activity of Acer tegmentosum Maxim on 3T3-L1 preadipocyte and high-fat diet-induced obese rats CURRENT REVIEW

Background: The aim of this study was to investigate the effect of Acer tegmentosum Maxim (ATM) on adipocyte differentiation in 3T3-L1 adipocyte-derived cells and antiobesity properties in high fat diet (HFD)-induced obese rats. Methods: 3T3-L1 adipocytes and HFD-induced obese rats were treated with ATM, and its effect on gene expression was analyzed using RT-PCR and Western blotting experiments. Results: Cellular lipid contents in DMI (dexamethasone, 3-isobutyl-1-methylxanthine, and insulin mixture)-treated cells increased, while ATM treatment caused a significant reduction in lipid accumulation in differentiated 3T3-L1 cells. ATM caused inhibition of adipogenesis via down-regulation of the CCAAT/enhancer binding protein β (C/EBPβ), C/EBPα, and peroxisome proliferator-activated receptor γ (PPARγ) expressions in 3T3-L1 cells. Moreover, treatment with ATM caused a decrease in the expressions of adipocyte-specific genes, such as adipocyte fatty acid-binding protein-2 (aP2), fatty acid synthase (FAS), and lipoprotein lipase (LPL), compared with DMI-stimulated adipocytes. In addition, phosphorylation levels of protein kinase B (Akt) and its downstream substrate, glycogen synthase kinase 3β (GSK3β), were significantly decreased by ATM treatment of 3T3-L1 adipocytes. Together, these results indicated that ATM caused inhibition of both adipocyte differentiation via suppression of the C/EBP family and PPARγ expressions and the Akt signaling pathway in 3T3-L1 adipocytes. In the present study, we further investigated antiobesity effects of ATM on HFD-induced obese rats. Rats fed with HFD demonstrated elevations in body weight gain, while the administration of ATM significantly reversed BW gains and adipose tissue weights in rats fed HFD. ATM supplementation also caused a decrease in the circulating triglyceride levels and total cholesterol the present study, we investigated the inhibitory effects of ATM on adipocyte differentiation in 3T3-L1 cell and the anti-obesity ATM activities on HFD-induced obesity rats was investigated by analyzing body and fat pad weights, adipocyte size, and blood biochemical profiles. lipid an hepatic lipogenesis, augmented lipid uptake, and/or TG of In the the liver tissue in the obese HFD-fed rats showed lipid droplet accumulation in hepatocytes, while the treatment with ATM caused a decrease in the deposits of hepatic lipids in liver tissue. These results indicate that the livers of ATM-treated rats had less adipocytes than the HFD-fed rats, thus inducing lipid droplet reduction in liver tissue. These findings highlight the critical role of ATM in abnormal lipid accumulation in liver tissue in addition to causing a reduction in

3 ATM-treated group was significantly smaller than that of HFD group. These results strongly demonstrate that ATM administration caused a reduction in adiposity via attenuation in adipose tissue mass and adipocyte size.
Conclusion: These finding demonstrated that ATM exerted anti-obesity effects through inhibition of adipocyte differentiation and adipogenesis, leading to a decrease in BW and fat tissue mass in HFD-induced obesity in rats.

Background
Obesity is a major risk factor of many metabolic disorders, including hyperlipidemia, type 2 diabetes, atherosclerosis, nonalcoholic fatty liver disease, and cardiovascular diseases [1]. Fat is properly defined as adipose tissue and is a biological caloric reservoir that expands in response to overnutrition and releases lipids in response to energy deficient conditions. Although adipose tissues are the major site for excess energy storage, excess adiposity and adipocyte dysfunction cause induction of increased levels of plasma free fatty acids and triglycerides (TGs), decreased levels of high-density lipoprotein (HDL), and abnormally low-density lipoprotein (LDL) composition in plasma and tissues, such as liver and muscle. These changes lead to pathological dysfunction of these tissues [2,3]. Also, it is important for an individual to maintain an adequate balance between energy accumulation and consumption in order to prevent and improve obesity.
Adipose cell hyperplasia (cell number increase) and hypertrophy (cell size increase) might be an important factor in obesity development. Adipocyte differentiation and intracellular lipid accumulation are associated with the occurrence and development of obesity [4].
Adipogenesis is a differentiation process by which undifferentiated preadipocytes are converted to fully differentiated adipocytes. Thus, inhibition of adipogenesis and adipocyte differentiation from fibroblast-like preadipocytes into fully mature adipocytes in addition to identifying critical factors that regulate these processes is extremely 4 important in an approach to primary obesity prevention and treatment.
The 3T3-L1 cell line is a well-established and widely used in vitro model of obesity for assessing adipocyte differentiation [5]. The differentiation process of 3T3-L1 cells into adipocytes follows a precisely ordered and temporal series of events regulated by a number of transcription factors and several signaling pathways for the development of the mature lipid-filled adipocytes [6,7]. During the differentiation process, the many key transcriptional factors that are involved, such as the CCAAT enhancer binding protein δ (C/EBPδ) and C/EBPβ, work in conjunction to induce peroxisome proliferator-activated receptor γ (PPARγ) and C/EBPα expressions [8]. C/EBPβ and C/EBPδ are expressed comparatively early in adipogenesis; this event is an important factor for initiating the transcriptional cascades that culminate in the expression of two essential adipogenic factors, PPARγ and C/EBPα [9]. PPARγ and C/EBPα are critical transcription factors involved in adipogenesis and regulatin of the genes, such as lipoprotein lipase (LPL), adipocyte fatty acid-binding protein-2 (aP2), and fatty acid synthase (FAS) [10,11], which are involved in adipogenesis and lipogenesis. Moreover, these transcription factors are also known to influence adipocyte differentiation in vivo.
The protein kinase B (Akt) signaling pathway plays a major role in adipocyte differentiation induced by a downstream function of insulin, and activation of the Akt pathway in 3T3-L1 preadipocytes contributes to adipocyte differentiation [12], whereas inactivation of the PI3K/Akt pathway inhibits adipogenesis [13]. Akt phosphorylates and regulates a number of substrates involved in a diverse array of biological process [14] and is essential for induction of PPARγ expression [15]. Moreover, overexpression of constitutively active Akt causes an increase in glucose uptake and adipocyte differentiation in 3T3-L1 adipocytes [16]. In contrast, the RNAi-mediated decrease in Akt expression blocks 3T3-L1 cells differentiation [17]. Adipocyte differentiation and lipid 5 accumulation is impaired in the absence of Akt, whereas Akt overexpression in Aktdeficient cells leads to lipid droplet accumulation [18].
Acer tegmentosum Maxim (ATM) is widely used as a traditional medicinal plant in Asia. A. tegmentosum has traditionally been used for treatment of various hepatic disorders, including hepatitis, cirrhosis, and liver cancer [19]. Phytochemical compounds isolated from A. tegmentosum has been reported to be one of the important sources of useful bioactive secondary metabolites, such as flavonoids, lignans, and phenolic compounds [20,21]. In addition, antioxidants, anti-inflammatory, and anti-lipogenic activities were also shown in biological investigations on ATM extracts [22,23]. However, the anti-obesity effects of ATM are still poorly understood.
In the present study, we examined the inhibitory effects of ATM on adipogenesis, its underlying molecular mechanisms in 3T3-L1 cells, and body weight reduction effects in high-fat diet (HFD)-induced obesity in rats.

Preparation of ATM
The Acer tegmentosum Maxim (ATM) stem was purchased from Jinju traditional herbal market (GyeongsangNam-do, Jinju, Korea). The dried and chopped twigs and inner barks of ATM was powdered and extracted with 70% ethanol at 60 °C for 72 h. The extract was filtered, dried under reduced pressure, and concentrated by an evaporator to obtain an ethanol-soluble fraction. Lyophilized ATM powder was dissolved in with dimethyl sulfoxide (DMSO, Sigma-Aldrich) at a concentration of 200 mg/mL and stored at -20 °C for further use.

Oil Red O staining and triglyceride assay
After the induction of differentiation, cells were stained with Oil Red O (60% isopropanol and 40% water), Briefly, the cells were gently washed with phosphate-buffered saline

RT-PCR
Cellular RNA was extracted from 3T3-L1 adipocytes using the Trizol reagent (Invitrogen, CA, USA) according to the manufacturer's instructions. One microgram of total RNA was used for first strand cDNA synthesis with oligo (deoxythymidine) primers and Superscript II 7 reverse transcriptase (Invitrogen, CA, USA). The target cDNA was amplified using the

Animal experiments
The study protocol was approved by the Ethic committee for Animal Experiments of Gyeongsang National University. All rats were handled according to the University Guidelines and the Animal Care Committee Guidelines of Gyeongsang National University (GNU-181231-R0066). 40 male Sprague-Dawley (SD) rats weighing approximately 180 g were purchased from the Central Lab. Animal Inc. (Seoul, Korea). Rats were acclimatized to the experimental facility for 1 week. All animals were kept under controlled temperature and on a 12 h light and 12 h dark cycle. The rats were divided into 3 groups of 10 and individually housed in polycarbonated cages in a room maintained at 22 °C and 55% relative humidity. All rats were allowed free access to their respective diets and drinking water for 5 weeks. Food intake was measured daily and body weight every two days. Obese rats were generated by feeding them a high fat diet (HFD). Normal diet group (ND) was maintained on a normal diet based on a commercial diet (#55VXT0038, Samyang Co, Korea). HFD group (HFD) was fed an HFD based on a commercial diet (rodent diet with 60% kcal fat, Research Diet, Korea). HFD plus ATM group (HFD+ATM) was fed an HFD, and ATM (150 mg/kg BW) was administered through the gastrointestinal tract.

Biochemical assays
After 5 weeks on experimental diets, the rats were anesthetized, and the tissues were dissected and analyzed. The body weight and fatty tissue mass were measured with sensitivity limits of 0.1 g and 0.01 g, respectively. The body weight index was calculated by dividing the weight (g) by the square of body length (cm2). Blood was isolated from each rat, stored at 37 °C for 30 min, and centrifuged at 4000 x g at 4 °C for 10 min to obtain plasma. The epididymal fat pad and perirenal fat pad were excised, weighed and stored at -20 °C until assayed. The concentrations of plasma triglyceride (TG), total cholesterol (TC), and high-density lipoprotein (HDL)-cholesterol were assayed enzymatically using commercial kits (Asan phams, Co., Korea).

Histological Analysis
The epididymal fat pads and liver tissues was removed and fixed in 10% neutral buffered formalin. The fat pads and livers were subsequently embedded in paraffin, sectioned into 5 μm sections (Leica, Wetzlar, Germany), and stained with hematoxylin-eosin for microscopic assessment (Olympus, Tokyo, Japan). Three different cross-sectional areas and their cell populations were calculated using an image analysis program (Image-Pro Plus 6.0).

Statistical analysis
All experiment results were reported as the means ± SD and the experiments were repeated three ~ four times. The differences between groups were determined using ANOVA and Duncan's multiple range tests. P < 0.05 was usually considered as statistically significant.

Effect of ATM on intracellular lipid accumulation and TG content in 3T3-L1 cells
The anti-adipogenic effects caused by ATM on preadipocyte differentiation into adipocytes were investigated. The degree of adipogenesis of 3T3- ATM strongly affected cellular lipid accumulation, which was decreased by approximately 40% in a dose-dependent manner (Fig. 1A). Intracellular TG levels were also measured on day 4 or 8 of 3T3-L1 adipogenic differentiation. The cellular TG content significantly increased in the DMI-treated group during 3T3-L1 differentiation for four or eight days, while the addition of ATM into the differentiation medium strongly blocked TG accumulation (Fig. 1B). Together, the results from Oil Red O staining and TG content assay demonstrated that ATM caused suppression of 3T3-L1 preadipocyte differentiation and decreased adipogenesis, intracellular lipid accumulation, and TG contents.

Effect of ATM on cell viability
The effects of ATM on cell viability were measured with the 3-(4,5 dimethylthiazol-2, 5 diphenyl) tetrazolium bromide (MTT) assay. 3T3-L1 cells were exposed to various concentrations (20~60 μg/mL) of ATM extracts for four or eight days. Results from the MTT assay showed that 24 h incubation of cells with the ATM extract produced no toxic effects on cell viability at concentrations up to 60 µg/mL (Fig. 1C). In this study, ATM concentrations in additional experiments were used up to 60 µg/mL.

Effect of ATM on the expression of adipogenic-specific genes during adipocyte differentiation
Adipogenesis was accompanied by an increase in adipogenic-related transcription factors and adipocyte-specific gene expression. In particular, C/EBPβ, C/EBPα, and PPARγ play a major role in adipogenesis. We examined whether ATM affected the mRNA levels related to expression of adopogenic transcription factors, such as C/EBPβ, C/EBPα, and PPARγ using reverse-transcriptase polymerase chain reaction (RT-PCR) during adipocyte differentiation.
Furthermore, the effects of ATM on the protein expression of the adipogenic transcription factors was examined using Western blotting analysis. C/EBPβ, C/EBPα, and PPARγ protein expression levels decreased in a dose-dependent manner in response to ATM in the 3T3-L1 adipocytes compared with those of untreated control cells (Fig. 2B). Our data indicate that ATM induced inhibition of adipogenesis transcription factor expression with subsequent blocking of adipocyte differentiation.
Effect of ATM on the expression of lipogenesis-specific genes during adipocyte differentiation Next, we investigated whether ATM could regulate the expression levels of lipogenesisspecific genes, such as FAS, LPL, and aP2. C/EBPα and PPARγ regulated the expression of their target genes, such as FAS, LPL, and aP2, during adipogenesis since those genes play a crucial role in determining and maintaining the mature adipocyte phenotype.
Differentiated 3T3-L1 adipocytes showed an increase in FAS, LPL, and aP2 expression levels, while treatment with ATM caused a significant, dose-dependent, down-regulation in the expression of these genes (Fig. 2C). These results indicate that ATM produces strong inhibition of lipid accumulation and causes a reduction in lipogenesis-specific gene expression levels in 3T3-L1 adipocytes.

Effect of ATM on the regulation of Akt and GSK3β during adipocyte differentiation
In order to investigate whether the Akt signaling pathway, a key regulator in adipogenesis, is activated by ATM during differentiation of 3T3-L1 cells, the phosphorylation level of Akt was examined after treatment with DMI alone or with DMI and ATM together. The results indicate that ATM treatment caused a significant and dosedependent inhibition of phosphorylated Akt levels (Fig. 3A). Moreover, ATM caused a reduction in the level of phosphorylated GSK3β, a downstream substrate of Akt (Fig. 3B).
These results demonstrate that activation of phosphorylated-Akt was inhibited by ATM; moreover, phosphorylation of GSK3β during adipogenesis in 3T3-L1 cells was also inhibited.

Effect of ATM on body weight and adipose tissue weight in HFD induced obesity rats
We further investigated the effects of ATM on body weight and adiposity in rats fed an HFD for five weeks. After one week of adaption, the rats used for this study were randomly distributed into three groups of ten rats: (1) a normal diet (ND) group; (2) high fat diet (HFD) group; and (3) HFD group with ATM (150 mg/kg/day; HFD+ATM). Body weight (BW) at five weeks significantly increased in rats in the HFD group compared with ND group, demonstrating that HFD fed rats developed into obese rats (Fig. 4A). The weight gains in the group of rats fed with the ATM extract significantly decreased compared with the HFD group. However, there were no significant differences in the amount of food intake in the HFD group compared with that of the other groups during the experimental period. Thus, ATM-induced suppression of body weight gain did not depend on reduction of food and water intake. We also found that rats treated with ATM exhibited lower perirenal and epididymal fat mass compared to HFD group (Fig. 4B), demonstrating that the reduction in body weight gain was primarily due to reduced lipid accumulation in adipocytes.

Effect of ATM on blood biochemical indexes in HFD-induced obese rats
In order to further investigate the anti-obesity effects of ATM, we estimated the changes in serum TG, TC, and HDL-C levels in HFD-induced obesity in rats. As shown in Fig 5, TG and TC levels were significantly higher in the HFD-fed rat group compared with those in the ND group (Fig. 5A). However, TG and TC levels in the HFD group significantly decreased by 36% and 30%, respectively, following ATM supplementation. Moreover, serum HDL-C levels in the ATM group significantly increased compared to those in the HFD group (Fig. 5A). Thus, the ATM extracts caused a significant decrease in serum TG and TC levels and increase in HDL-C levels.

Effect of ATM on lipid accumulation in epidydymal adipose tissue
Next, in order to investigate whether the epididymal fat mass reduction in the ATMtreated group led to a decrease in epididymal adipose tissue cell size in obese rats, histological analyses of epididymal adipose tissue was performed. As shown in Fig. 5, hematoxylin and eosin (H&E) staining results for epididymal tissue from each group showed that lipid accumulation significantly increased in the HDF compared with ND group (Fig. 5B). However, the size of the adipocytes in the ATM administration group in the epididymal fat was significantly smaller than in the HFD-fed rats (Fig. 5B), and the size of adipocytes in the epididymal adipose tissue was reduced by 35% in ATM-treated rats.

Effect of ATM in the fatty lever in indexes in HFD-induced obese rats
Body weights of HFD-fed rats significantly increased in comparison to control rats after five weeks of feeding initiation. The HFD-fed rats exhibited obesity-related characteristics in terms of body weight, weight gain, and food intake compared to those in the ND group at the end of the study. H&E staining showed that the number of lipid droplets increased in the liver of rats undergoing HFD-induced obesity. Hepatic steatosis and ballooning degeneration of hepatocytes in the liver tissues were higher in the HFD than the ND group ( Fig. 5C). However, ATM administration significantly blocked the effects of HFD-induced hepatic steatosis (Fig. 5C).

Discussion
Obesity is a medical condition in which abnormal or excessive fat accumulates mainly due to sedentary behavior that is linked to lower health-related quality of life, lack of exercise, and intake of foods rich in fats and oils. Trends in overweight prevalence have been increasing rapidly in children and adults and are associated with serious mortalities, including a high hyperlipidemia incidence, type 2 diabetes mellitus, insulin resistance, heart disease, and fatty liver in addition to various types of cancer and osteoarthritis [1].
14 The currently available treatment options are not potent enough to prevent obesity permanently, and other effective strategies for weight loss may cause other side effects.
Therefore, there is a great demand for long-term use of complementary, alternative and safe and effective medicines to treat this pandemic global obesity problem.
In the present study, we investigated the inhibitory effects of ATM on adipocyte differentiation in 3T3-L1 cell and the anti-obesity ATM activities on HFD-induced obesity rats was investigated by analyzing body and fat pad weights, adipocyte size, and blood biochemical profiles.
ATM is well-known as a functional tree with potential medicinal benefits. Diarylheptanoids [24], rhododendrol glycoside [25], and tannins [26] have been isolated from the genus Acer. Other studies have also demonstrated that ATM is rich in abundant functional compounds, including polyphenols, phenethyl glycosides, and flavonoids, suggesting that these compounds exhibit hepatoprotective activities [20,21]. ATM exhibits potent antiangiogenic activity both in vivo and in vitro [27] and has been shown to be cytotoxic to cancer cells [28].
These results are the first to demonstrate that ATM causes inhibition of differentiation and adipogenesis of 3T3-L1 preadipocytes and lipid accumulation in mature differentiated adipocytes via regulation of adipogenic transcription factor expression and Akt signaling.
In order to investigate whether ATM affects adipocyte differentiation, we measured the effects of ATM on lipid accumulation during 3T3-L1 preadipocyte differentiation.
Adipogenesis is defined as the process by which preadipocytes differentiate into adipocytes. 3T3-L1 cells are induced to differentiate into adipocytes according to a coordinated program and are one of the most useful cell lines for studying adipogenesis [29]. In the present study, lipid accumulation was measured by Oil Red O staining and the TG assay. Our data demonstrated that ATM treatment caused a reduction in TG levels and In line with these findings, we examined the effects of ATM on adipogenesis and lipid accumulation in 3T3-L1 adipocytes via regulation of adipogenic gene expression.
Differentiation of preadipocytes is regulated by a complex network of transcription factors, mainly consisting of the C/EBP family and PPARγ. This cooperative function helps to maintain each of their own high expression levels. Moreover, adipocytes from mice in which the C/EBPα gene was disrupted showed defects in lipid accumulation [30].
Transgenic mice specifically lacking PPARγ in adipose tissue exhibited greatly reduced fat pad sizes [31]. PPARγ stimulates adipocytes and induces differentiation, while C/EBPα also supports the adipocyte differentiation process. Therefore, low levels of these adipocyte differentiation-specific genes are important markers of adipocyte inhibition.
In the present study, the treatment with ATM caused a decrease in mRNA expression of C/EBPβ, C/EBPα, and PPARγ in 3T3-L1 adipocytes. Western blot analysis showed that C/EBPβ, C/EBPα, and PPARγ protein levels during adipogenesis of 3T3-L1 preadipocytes demonstrated a dose-dependent reduction. These results show that the ATM treatment caused inhibition of adipocyte differentiation via inhibition of PPARγ and C/EBP familyassociated adipogenesis signaling, and ATM effects on adipogenic transcription factors involved in adipocyte differentiation in 3T3-L1 cells play a critical role in adipogenesis mediation.
It is well known that adipocyte differentiation is associated with multifunctional cellular pathways and requires sequential regulation of adipogenic and lipogenic genes [32].
PPARγ and C/EBPα activate gene expressions, such as aP2, LPL, FAS and others, that are involved in adipogenesis and lipogenesis and participate in creating the adipocyte phenotype [10,11,33].
In the present study, the effects of ATM on the expression of aP2, FAS, and LPL were investigated during 3T3-L1 differentiation. The expression of aP2, FAS, and LPL genes is significantly down-regulated in a dose-dependent manner following ATM treatment.
Together, our results demonstrate that ATM led to strong suppression of the expression of critical genes involved in creating and maintaining adipogenesis and lipogenesis via lipid storage and accumulation in 3T3-L1 adipocytes.
Several lines of evidence support the concept that the lipogenic pathway is localized to peroxisomes and is important for endogenous activation of PPARγ. PPARγ mainly regulates the gene network expression involved in adipogenesis and lipid metabolism [34]. PPARγ has a major role in the differentiation of preadipocytes into adipocytes involved in obesity development [35,36]. The fact that PPARγ null mice complete lack adipose tissue clearly demonstrates that PPARγ is essential for adipocyte differentiation [37]. Focusing on lipid metabolism genes, FAS regulates fatty acid synthesis from acetyl-and malonyl-CoA and via the tricarboxylic acid cycle [38]. Therefore, reduction of FAS via ATM treatment could explain the way in which ATM extracts suppress lipid accumulation. aP2 is highly expressed in adipose tissue and plays a lipid binding protein, which reacts as a key factor in intracellular fatty acid transport and lipid metabolism [10,11]. The expression of lipoprotein lipase (LPL) is stimulated by PPARγ, thus increasing fatty acid uptake into adipocytes [11]. These genes are activated in response to PPARγ regulation [10,11]. In this study, ATM caused a reduction in the expressed level of FAS, LPL, and aP2, suggesting that intracellular fatty acid transport and lipid metabolism are reduced due to low lipid accumulation. The expression levels of those adipogenic and lipogenic-related genes decreased in ATM-treated 3T3-L1 adipocytes. These studies explain ATM-induced inhibition of adipocyte differentiation and lipogenesis in 3T3-L1 preadipocytes. Therefore, ATM reduced adipogenesis and lipid synthesis within differentiated adipocytes at the gene level through inhibition of genes involved in adipocyte lipogenesis and fatty acid transport and synthesis, thus leading to lipogenesis.
Akt kinases play an important role in adipogenesis and glucose transport [39]. Inhibition of Akt activation in fibroblasts displays lack of capability to differentiate preadipocytes into adipocytes, and Akt overexpression results in an increase in glucose uptake and adipocyte differentiation [40]. In the present study, our results demonstrate that ATM caused a significant and concentration-dependent decrease in the phosphorylation of Akt in 3T3-L1 adipocytes. In addition, treatment with ATM caused a marked decreased in phosphorylation of GSK3β during adipocyte differentiation of the 3T3-L1 preadipocytes. It is known that Akt phosphorylation can promote adipocyte differentiation via PPARγ upregulation [41]. The Akt signaling cascade is considered important for adipogenesis as it appears to activate PPARγ and C/EBPα during induction of 3T3-L1 adipocyte differentiation [41]. Xu et al. showed that Akt activation enhances an important association between the Akt signaling cascade and transcription factors, PPARγ and C/EBPα, in induction of 3T3-L1 adipocyte differentiation [42]. Taken together, these results demonstrate that ATM suppressed adipocyte differentiation and lipid accumulation in 3T3-L1 cells via downregulation of adipogenic transcription factors and the Akt signaling pathway associated with intracellular lipid accumulation.
Rodent models of HFD-induced obesity have been widely used to investigate human obesity and its related metabolic diseases. Five weeks of an HFD leads to overt obesity in rats and is characterized by body weight gain along with an increase of fat tissue weight.
In this animal study, we established that HFD-induced obesity in rats caused an increase in body weight of rats and serum TG and TC levels and a decrease in HDL-C levels.
However, our results showed that BW gain was lower in rats administrated the ATM extract. The weights of epididymal and perirenal adipose tissue were also markedly reduced in rats supplemented with ATM, indicating that BW loss is mainly due to decreased fat accumulation in epididymal and perirenal adipose tissues. We also observed that serum TG and TC were lower in rats fed HFD plus ATM than those of rats fed HFD, indicating that ATM is beneficial for improvements in numerous serum metabolic parameters. Thus, these data suggest that ATM suppressed lipid disorders caused by the excess lipid found in HFD-induced obese rats, thereby improving hypertriglyceridemia and hypercholesterolemia caused by HFD-induced obesity. In addition, increased adipose tissue mass, particularly the amount of visceral fat, is associated with an increased risk of metabolic diseases. In this study, ATM-fed rats showed lower masses of epididymal and perirenal adipose tissues. Thus, supplementation with ATM caused reduction in BW and fat tissue weights, thus preventing fat accumulation and improving to obesity.
Next we investigated the effect of ATM extract for the prevention on fatty liver. The liver is mostly regarded as an essential organ in lipid metabolism. Imbalance between lipid deposition and removal results in hepatic lipid accumulation, which is related to an increase in hepatic lipogenesis, augmented lipid uptake, and/or reduced TG export of βoxidation products (43). In the present study, the liver tissue in the obese HFD-fed rats showed lipid droplet accumulation in hepatocytes, while the treatment with ATM caused a decrease in the deposits of hepatic lipids in liver tissue. These results indicate that the livers of ATM-treated rats had less adipocytes than the HFD-fed rats, thus inducing lipid droplet reduction in liver tissue. These findings highlight the critical role of ATM in reducing abnormal lipid accumulation in liver tissue in addition to causing a reduction in 19 BW gain.

Conclusions
In this study, we showed the inhibitory effects of ATM on adipocyte differentiation. ATM