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Research
Xiangdong Li
China Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2261-2393
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Background
Type 2 diabetes mellitus (T2DM) is the most common type of metabolic disorder involving multiple organ systems. Grape has been reported to improve the symptoms of T2DM, the precise mechanism of its action is unclear. Our study was aimed to determine the effect and mechanism of grape pomace extract in T2DM mice induced by high fat diet (HFD).
Materials and methods
Ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry were used to identify the main active compounds in grape pomace extract to improve T2DM. C57BLK/6J mice induced by HFD supplemented with or without quercetin were used to show the effects of quercetin improving T2DM. By online database research, bioinformatics analysis and molecular biology experiments, Estrogen receptor alpha (ERα)-lncSHGL (lncRNA suppressor of hepatic gluconeogenesis and lipogenesis) pathway was identify as the target for quercetin.
Results
Quercetin was identified as one of the most active compounds in grape pomace extract to improve T2DM. Quercetin could inhibit HFD-induced T2DM in mice by activing ERα. LncSHGL was identified as the downstream of ERα and inhibited HFD-induced T2DM.
Conclusions
Quercetin could be beneficial for T2DM by promoting lncSHGL transcription and activating the lncSHGL pathway, and may be used as a drug component to treat T2DM.
Keywords
grape pomace extract, quercertin, T2DM, ERα, lncSHGL, heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1)
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Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 24 Feb, 2020
No community comments so far
Editorial decision: Minor revision
On 23 Apr, 2020
Review #2 received
Received 30 Mar, 2020
Review #1 received
Received 30 Mar, 2020
Reviewer #2 agreed
On 17 Mar, 2020
Reviewers invited
Invitations sent on 10 Mar, 2020
Reviewer #1 agreed
On 10 Mar, 2020
Editor assigned
On 26 Feb, 2020
Editor invited
On 25 Feb, 2020
Submission checks complete
On 21 Feb, 2020
First submitted
On 20 Feb, 2020
Metrics
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HTML Views: ...
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This preprint is under consideration at Nutrition & Metabolism. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Xiangdong Li
China Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2261-2393
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 24 Feb, 2020
No community comments so far
Editorial decision: Minor revision
On 23 Apr, 2020
Review #2 received
Received 30 Mar, 2020
Review #1 received
Received 30 Mar, 2020
Reviewer #2 agreed
On 17 Mar, 2020
Reviewers invited
Invitations sent on 10 Mar, 2020
Reviewer #1 agreed
On 10 Mar, 2020
Editor assigned
On 26 Feb, 2020
Editor invited
On 25 Feb, 2020
Submission checks complete
On 21 Feb, 2020
First submitted
On 20 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Type 2 diabetes mellitus (T2DM) is the most common type of metabolic disorder involving multiple organ systems. Grape has been reported to improve the symptoms of T2DM, the precise mechanism of its action is unclear. Our study was aimed to determine the effect and mechanism of grape pomace extract in T2DM mice induced by high fat diet (HFD).
Materials and methods
Ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry were used to identify the main active compounds in grape pomace extract to improve T2DM. C57BLK/6J mice induced by HFD supplemented with or without quercetin were used to show the effects of quercetin improving T2DM. By online database research, bioinformatics analysis and molecular biology experiments, Estrogen receptor alpha (ERα)-lncSHGL (lncRNA suppressor of hepatic gluconeogenesis and lipogenesis) pathway was identify as the target for quercetin.
Results
Quercetin was identified as one of the most active compounds in grape pomace extract to improve T2DM. Quercetin could inhibit HFD-induced T2DM in mice by activing ERα. LncSHGL was identified as the downstream of ERα and inhibited HFD-induced T2DM.
Conclusions
Quercetin could be beneficial for T2DM by promoting lncSHGL transcription and activating the lncSHGL pathway, and may be used as a drug component to treat T2DM.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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