Association of MUC1 5640G>A and PSCA 5057C>T polymorphisms with the Risk of Gastric Cancer in Northern Iran
Background: Gastric cancer is the fourth most common cancer worldwide and the second leading cause of cancer-related death. Genome-Wide Association Studies (GWAS) have shown that genetic diversities MUC1 (Mucin 1) and PSCA (Prostate Stem Cell Antigen) genes are involved in gastric cancer. The aim of this study was to determine the association between rs4072037G>A polymorphism in MUC1 and rs2294008 C>T in PSCA gene with gastric cancer in northern Iran.
Methods: DNA was extracted from 99 formalin fixed paraffin-embedded (FFPE) tissue samples of gastric cancer and 96 blood samples from healthy individuals (sex matched) as controls. DNA was extracted followed by PCR using specific primers. Two desired polymorphisms, 5640G>A and 5057C>T for MUC1 and PSCA genes were genotyped using PCR-RFLP method.
Results: The G allele at rs4072037 of MUC1 gene was associated with a significant decreased gastric cancer risk (OR = 0.507, 95% CI: 0.322–0.799, p = 0.003). A significant decreased risk of gastric cancer was observed in people with either AG vs. AA, AG+AA vs. GG and AA+GG vs. AG genotypes of MUC1 polymorphism (OR = 4.296, 95% CI: 1.190–15.517, p = 0.026), (OR = 3.726, 95% CI: 2.033–6.830, p = 0.0001) and (OR = 0.223, 95% CI: 0.120-0.413, p = 0.0001) respectively. Overall, no significant association was observed between the PSCA 5057C>T polymorphism and risk of gastric cancer in all genetic models.
Conclusion: Results indicated that the MUC1 5640G>A polymorphism may have protective effect for gastric cancer and could be considered a potential molecular marker in gastric cancer in the Northern Iran population.
Figure 1
Posted 16 Jun, 2020
On 13 Jul, 2020
On 15 Jun, 2020
On 15 Jun, 2020
On 14 Jun, 2020
On 14 Jun, 2020
On 26 May, 2020
Received 25 May, 2020
On 07 May, 2020
Received 30 Apr, 2020
Invitations sent on 29 Apr, 2020
On 29 Apr, 2020
On 27 Apr, 2020
On 26 Apr, 2020
On 20 Feb, 2020
On 06 Apr, 2020
Received 21 Mar, 2020
Received 17 Mar, 2020
On 03 Mar, 2020
On 02 Mar, 2020
On 23 Feb, 2020
Invitations sent on 23 Feb, 2020
On 21 Feb, 2020
On 20 Feb, 2020
Association of MUC1 5640G>A and PSCA 5057C>T polymorphisms with the Risk of Gastric Cancer in Northern Iran
Posted 16 Jun, 2020
On 13 Jul, 2020
On 15 Jun, 2020
On 15 Jun, 2020
On 14 Jun, 2020
On 14 Jun, 2020
On 26 May, 2020
Received 25 May, 2020
On 07 May, 2020
Received 30 Apr, 2020
Invitations sent on 29 Apr, 2020
On 29 Apr, 2020
On 27 Apr, 2020
On 26 Apr, 2020
On 20 Feb, 2020
On 06 Apr, 2020
Received 21 Mar, 2020
Received 17 Mar, 2020
On 03 Mar, 2020
On 02 Mar, 2020
On 23 Feb, 2020
Invitations sent on 23 Feb, 2020
On 21 Feb, 2020
On 20 Feb, 2020
Background: Gastric cancer is the fourth most common cancer worldwide and the second leading cause of cancer-related death. Genome-Wide Association Studies (GWAS) have shown that genetic diversities MUC1 (Mucin 1) and PSCA (Prostate Stem Cell Antigen) genes are involved in gastric cancer. The aim of this study was to determine the association between rs4072037G>A polymorphism in MUC1 and rs2294008 C>T in PSCA gene with gastric cancer in northern Iran.
Methods: DNA was extracted from 99 formalin fixed paraffin-embedded (FFPE) tissue samples of gastric cancer and 96 blood samples from healthy individuals (sex matched) as controls. DNA was extracted followed by PCR using specific primers. Two desired polymorphisms, 5640G>A and 5057C>T for MUC1 and PSCA genes were genotyped using PCR-RFLP method.
Results: The G allele at rs4072037 of MUC1 gene was associated with a significant decreased gastric cancer risk (OR = 0.507, 95% CI: 0.322–0.799, p = 0.003). A significant decreased risk of gastric cancer was observed in people with either AG vs. AA, AG+AA vs. GG and AA+GG vs. AG genotypes of MUC1 polymorphism (OR = 4.296, 95% CI: 1.190–15.517, p = 0.026), (OR = 3.726, 95% CI: 2.033–6.830, p = 0.0001) and (OR = 0.223, 95% CI: 0.120-0.413, p = 0.0001) respectively. Overall, no significant association was observed between the PSCA 5057C>T polymorphism and risk of gastric cancer in all genetic models.
Conclusion: Results indicated that the MUC1 5640G>A polymorphism may have protective effect for gastric cancer and could be considered a potential molecular marker in gastric cancer in the Northern Iran population.
Figure 1