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Research article
Mohammad Bagher Hashemi-Soteh
Immunogenetic Research Center, Molecular and Biology Research Center, Medical Faculty, Mazandaran University of Medical, Sari, mazandaran, Iran
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5361-520X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Gastric cancer is the fourth most common cancer worldwide and the second leading cause of cancer-related death. Genome-Wide Association Studies (GWAS) have shown that genetic diversities MUC1 (Mucin 1) and PSCA (Prostate Stem Cell Antigen) genes are involved in gastric cancer. The aim of this study was to determine the association between rs4072037G>A polymorphism in MUC1 and rs2294008 C>T in PSCA gene with gastric cancer in northern Iran.
Methods: DNA was extracted from 99 formalin fixed paraffin-embedded (FFPE) tissue samples of gastric cancer and 96 blood samples from healthy individuals (sex matched) as controls. DNA was extracted followed by PCR using specific primers. Two desired polymorphisms, 5640G>A and 5057C>T for MUC1 and PSCA genes were genotyped using PCR-RFLP method.
Results: The G allele at rs4072037 of MUC1 gene was associated with a significant decreased gastric cancer risk (OR = 0.507, 95% CI: 0.322–0.799, p = 0.003). A significant decreased risk of gastric cancer was observed in people with either AG vs. AA, AG+AA vs. GG and AA+GG vs. AG genotypes of MUC1 polymorphism (OR = 4.296, 95% CI: 1.190–15.517, p = 0.026), (OR = 3.726, 95% CI: 2.033–6.830, p = 0.0001) and (OR = 0.223, 95% CI: 0.120-0.413, p = 0.0001) respectively. Overall, no significant association was observed between the PSCA 5057C>T polymorphism and risk of gastric cancer in all genetic models.
Conclusion: Results indicated that the MUC1 5640G>A polymorphism may have protective effect for gastric cancer and could be considered a potential molecular marker in gastric cancer in the Northern Iran population.
Keywords
Gastric cancer, Genome-Wide Association Studies (GWAS), rs4072037G>A polymorphism, rs2294008 C>T
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Received 30 Apr, 2020
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Editor invited
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Editorial decision: Major revision
On 06 Apr, 2020
Review #2 received
Received 21 Mar, 2020
Review #1 received
Received 17 Mar, 2020
Reviewer #2 agreed
On 03 Mar, 2020
Reviewer #1 agreed
On 02 Mar, 2020
Editor assigned
On 23 Feb, 2020
Reviewers invited
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See the published version of this preprint at BMC Medical Genetics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Mohammad Bagher Hashemi-Soteh
Immunogenetic Research Center, Molecular and Biology Research Center, Medical Faculty, Mazandaran University of Medical, Sari, mazandaran, Iran
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5361-520X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Medical Genetics.
Version 3
Posted 16 Jun, 2020
No community comments so far
Editor assigned
On 15 Jun, 2020
Editorial decision: Minor revision
On 15 Jun, 2020
Submission checks complete
On 14 Jun, 2020
Editor invited
On 14 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 26 May, 2020
Review #2 received
Received 25 May, 2020
Reviewer #2 agreed
On 07 May, 2020
Review #1 received
Received 30 Apr, 2020
Reviewers invited
Invitations sent on 29 Apr, 2020
Reviewer #1 agreed
On 29 Apr, 2020
Editor assigned
On 27 Apr, 2020
Editor invited
On 26 Apr, 2020
Submission checks complete
On 20 Feb, 2020
No comments provided
Editorial decision: Major revision
On 06 Apr, 2020
Review #2 received
Received 21 Mar, 2020
Review #1 received
Received 17 Mar, 2020
Reviewer #2 agreed
On 03 Mar, 2020
Reviewer #1 agreed
On 02 Mar, 2020
Editor assigned
On 23 Feb, 2020
Reviewers invited
Invitations sent on 23 Feb, 2020
Submission checks complete
On 21 Feb, 2020
Editor invited
On 20 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Medical Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Medical Genetics.
Background: Gastric cancer is the fourth most common cancer worldwide and the second leading cause of cancer-related death. Genome-Wide Association Studies (GWAS) have shown that genetic diversities MUC1 (Mucin 1) and PSCA (Prostate Stem Cell Antigen) genes are involved in gastric cancer. The aim of this study was to determine the association between rs4072037G>A polymorphism in MUC1 and rs2294008 C>T in PSCA gene with gastric cancer in northern Iran.
Methods: DNA was extracted from 99 formalin fixed paraffin-embedded (FFPE) tissue samples of gastric cancer and 96 blood samples from healthy individuals (sex matched) as controls. DNA was extracted followed by PCR using specific primers. Two desired polymorphisms, 5640G>A and 5057C>T for MUC1 and PSCA genes were genotyped using PCR-RFLP method.
Results: The G allele at rs4072037 of MUC1 gene was associated with a significant decreased gastric cancer risk (OR = 0.507, 95% CI: 0.322–0.799, p = 0.003). A significant decreased risk of gastric cancer was observed in people with either AG vs. AA, AG+AA vs. GG and AA+GG vs. AG genotypes of MUC1 polymorphism (OR = 4.296, 95% CI: 1.190–15.517, p = 0.026), (OR = 3.726, 95% CI: 2.033–6.830, p = 0.0001) and (OR = 0.223, 95% CI: 0.120-0.413, p = 0.0001) respectively. Overall, no significant association was observed between the PSCA 5057C>T polymorphism and risk of gastric cancer in all genetic models.
Conclusion: Results indicated that the MUC1 5640G>A polymorphism may have protective effect for gastric cancer and could be considered a potential molecular marker in gastric cancer in the Northern Iran population.
Figure 1
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