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Negar Azarpira
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Background
Tissue engineering is considered as a promising tool for remodeling the native cells microenvironment. In the present study, the effect of alginate hydrogel and collagen microspheres integrated with extracellular matrix components were evaluated in the decrement of apoptosis in human pancreatic islets.
Methods
For three dimensional culture, the islets were encapsulated in collagen microspheres, containing laminin and collagen IV and embedded in alginate scaffold for one week. After that the islets were examined in terms of viability, apoptosis, genes and proteins expression including BAX; BCL2; active caspase-3; and insulin. Moreover, the islets function was evaluated through the glucose-induced insulin and C-peptide secretion assay. In order to evaluate the scaffolds structure and the pancreatic islets morphology in three-dimensional microenvironments, scanning electron microscopy was done.
Results
Our findings showed that the designed hydrogel scaffolds significantly improved the islets viability the activated caspase-3 and TUNEL positive cells were clearly reduced.
Conclusion
The reduced pancreatic islet survival and function occurs, following the degradation of extracellular matrix and the stresses introduced into the cells during isolation and culture. The reconstruction of the destructed matrix with alginate hydrogels and collagen microspheres might be an effective step to promote the culture and transplantation of the islets.
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Negar Azarpira
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
View the published article at Acta Histochemica.
No community comments so far
Version 1
Posted 24 Feb, 2020
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Stem Cell & Developmental Cell Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Acta Histochemica.
Background
Tissue engineering is considered as a promising tool for remodeling the native cells microenvironment. In the present study, the effect of alginate hydrogel and collagen microspheres integrated with extracellular matrix components were evaluated in the decrement of apoptosis in human pancreatic islets.
Methods
For three dimensional culture, the islets were encapsulated in collagen microspheres, containing laminin and collagen IV and embedded in alginate scaffold for one week. After that the islets were examined in terms of viability, apoptosis, genes and proteins expression including BAX; BCL2; active caspase-3; and insulin. Moreover, the islets function was evaluated through the glucose-induced insulin and C-peptide secretion assay. In order to evaluate the scaffolds structure and the pancreatic islets morphology in three-dimensional microenvironments, scanning electron microscopy was done.
Results
Our findings showed that the designed hydrogel scaffolds significantly improved the islets viability the activated caspase-3 and TUNEL positive cells were clearly reduced.
Conclusion
The reduced pancreatic islet survival and function occurs, following the degradation of extracellular matrix and the stresses introduced into the cells during isolation and culture. The reconstruction of the destructed matrix with alginate hydrogels and collagen microspheres might be an effective step to promote the culture and transplantation of the islets.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
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