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Zhang Yuan
Chongqing Medical University
Corresponding Author
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Background: MSCs have been proven to have immune modulation and anti-inflammation capabilities, but the mechanisms are still under investigation. Recently, oxylipins have been identified to be involved in the immuno-regulation function of MSCs. However, the impact of proinflammatory stimulation on the profiles of MSCs derived oxylipins has never been studied.
Methods: In the present research, we employed a newly developed UPLC-MS/MS method to identify and quantify the oxylipin profile of ADSCs under proinflammatory stimulation (TNF-α and IFN-𝛾).
Results: As a result, among the analyzed 71 oxylipins, we detected and quantified 49 oxylipins derived from six major n-6 and n-3 polyunsaturated fatty acids (PUFAs) including arachidonic (AA), linoleic (LA), alpha-linolenic (ALA), dihomo-𝛾-linolenic acid (DGLA), eicosapentaenoic (EPA), and docosahexaenoic (DHA) acids in all samples, while 4 oxylipins were detected only in a part of samples and 18 were not detected in all samples. Nine oxylipins were found to be significantly differentially produced in ADSCs after 24 hours of inflammatory stimulation, among which, several oxylipins were reported to be having anti-inflammation or proresolving functions and involved in lipid mediator switching.
Conclusions: The results reported here make a fundamental step towards a comprehensive characterization of MSCs derived oxylipins as potential modulators of inflammation and immunoreaction.
Keywords
Oxylipins, Mesenchymal stem cells (MSCs), Lipidomics, Inflammation
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This is a list of supplementary files associated with this preprint. Click to download.
- TableS11009.pdf
Supplementary table S1. Standard calibration curve of 71 target lipid metabolites. The peak area ratio between the analyte and its respective internal standard were used (“y”) and the concentrations (“x”) were calculated using the calibration curve equation. LLOQ（nMol/L） and ULOQ（nMol/L） represent lower and upper limit of quantification respectively.
- TableS21009.pdf
Supplementary table S2. Quantification results of ADSCs derived oxylipins (nMol/sample). M1, M2 and M3 refer to ADSC samples without stimulation, while TI1, TI2 and TI3 refer to ADSC samples with stimulation of recombinant human IFN-γ (20 ng/ml) and TNF-α (20 ng/ml), n = 3 experimental replicates per group. N/A= below the limits of detection.
- TableS31009.pdf
Supplementary table S3. A list of 41 metabolic pathway involved in differentially expressed oxylipins was analyzed using the HMDB database.
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Zhang Yuan
Chongqing Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
The most recent version of this article is available here.
No community comments so far
Version 1
Posted 19 Jan, 2021
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Cell Biology & Physiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Background: MSCs have been proven to have immune modulation and anti-inflammation capabilities, but the mechanisms are still under investigation. Recently, oxylipins have been identified to be involved in the immuno-regulation function of MSCs. However, the impact of proinflammatory stimulation on the profiles of MSCs derived oxylipins has never been studied.
Methods: In the present research, we employed a newly developed UPLC-MS/MS method to identify and quantify the oxylipin profile of ADSCs under proinflammatory stimulation (TNF-α and IFN-𝛾).
Results: As a result, among the analyzed 71 oxylipins, we detected and quantified 49 oxylipins derived from six major n-6 and n-3 polyunsaturated fatty acids (PUFAs) including arachidonic (AA), linoleic (LA), alpha-linolenic (ALA), dihomo-𝛾-linolenic acid (DGLA), eicosapentaenoic (EPA), and docosahexaenoic (DHA) acids in all samples, while 4 oxylipins were detected only in a part of samples and 18 were not detected in all samples. Nine oxylipins were found to be significantly differentially produced in ADSCs after 24 hours of inflammatory stimulation, among which, several oxylipins were reported to be having anti-inflammation or proresolving functions and involved in lipid mediator switching.
Conclusions: The results reported here make a fundamental step towards a comprehensive characterization of MSCs derived oxylipins as potential modulators of inflammation and immunoreaction.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
- TableS11009.pdf
Supplementary table S1. Standard calibration curve of 71 target lipid metabolites. The peak area ratio between the analyte and its respective internal standard were used (“y”) and the concentrations (“x”) were calculated using the calibration curve equation. LLOQ（nMol/L） and ULOQ（nMol/L） represent lower and upper limit of quantification respectively.
- TableS21009.pdf
Supplementary table S2. Quantification results of ADSCs derived oxylipins (nMol/sample). M1, M2 and M3 refer to ADSC samples without stimulation, while TI1, TI2 and TI3 refer to ADSC samples with stimulation of recombinant human IFN-γ (20 ng/ml) and TNF-α (20 ng/ml), n = 3 experimental replicates per group. N/A= below the limits of detection.
- TableS31009.pdf
Supplementary table S3. A list of 41 metabolic pathway involved in differentially expressed oxylipins was analyzed using the HMDB database.
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