Activation of hepatic stellate cells (HSCs) is the most important factor in the hepatic fibrosis progression. Autophagy is closely related to the activation of HSCs. Based on our previous works, this research tried to explore the anti-fibrotic mechanism of combination therapy with Taurine, Epigallocatechin gallate（EGCG） and Genistein, referred to as TEG, by monitored autophagy in HSCs and CCl4-induced liver fibrosis rats. Transmission electron microscopy showed that there were many autophagosomes and autophagy lysosomes in HSCs and fibrotic rats. After TEG intervention, autophagosomes are decreased, HSCs proliferation is inhibited and liver tissue damage is repaired. Western blot and IHC results found that TEG reduced the expression of autophagy marker proteins LC3-II and Beclin1 in HSCs or fibrotic rats. mRFP-GFP-LC3 showed that green fluorescence increased after TEG treat HSCs. AMPK-mTOR pathway analysis revealed that TEG inhibited LC3, Beclin1, AMPK(Adenosine 5'-monophosphate (AMP)-activated protein kinase) and mTOR genes and phosphorylated protein expression in HSCs. Our results indicate that TEG anti-fibrotic may be associated with reduce autophagosomes by inhibit AMPK-mTOR pathway.