During the hot stamping process, heat exchange always occurs between the hot sheet and cold tools. The interfacial heat transfer in the whole forming process will not only affects the forming quality of the parts but also partially determines their post-form mechanical properties and microstructure distribution. As an essential parameter, the interface heat transfer coefficient (IHTC) is of great significance for the prediction of temperature fields in the finite element simulations, especially for the novel forming process named triple-layer sheet hot stamping. In this study, the triple-layer sheet heat exchange experiment is carried out to investigate the interfacial heat transfer behavior of the titanium alloy sheet in the triple-layer sheet hot stamping process. The effects of contact pressure, cladding steel sheet thickness, and contact gap on the interfacial heat transfer behavior and post-form mechanical properties of titanium alloy sheet are analyzed, and the interface heat transfer coefficient between the titanium alloy and high-strength steel sheets is calculated. The finite element model of triple-layer sheet heat transfer is established to verify the accuracy of the calculated interfacial heat transfer coefficient. The results show that the upper and lower steel sheets maintain the temperature of the titanium alloy sheets at a higher level in the triple-layer sheet hot stamping compared with the single-layer one. The temperature of the titanium alloy sheet under the single-layer hot stamping decreases from 900°C to 781.6°C after transferring, a decrease of 118.4°C. The temperature of the titanium alloy sheet only declines by 57.5°C during this period in the triple-layer sheet hot stamping process. In addition, the titanium alloy parts obtained by the triple-layer sheet hot stamping process have better mechanical properties. The IHTC increases with the increase of contact pressure and decreases with the increase of steel sheet thickness and contact gap. The accuracy of the calculated IHTC between the titanium alloy and cladding steel sheets is validated by comparing the results of the experiment and simulation. The maximum error between the simulation results and the experimental measurements is 5.5%.