Controlled release of vitamin D 3 using a nanocellulose-based membrane

doi:10.21203/rs.3.rs-1519306/v1

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Controlled release of vitamin D 3 using a nanocellulose-based membrane

https://doi.org/10.21203/rs.3.rs-1519306/v1

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Background

Epidemiological studies show that a significant fraction of the global population presents low levels of vitamin D₃. In order to address this problem, one way to administer the vitamin is to incorporate it in novel drug delivery systems, such as transdermal devices. A possible substance for this purpose is cellulose, which has a long history of use in the health area. However, the application of nanostructured cellulose membranes, as local drug delivery systems, remains a challenge.

Objective

To develop a crystalline nanocellulose membrane as a new tool for the release of vitamin D₃.

Methods

A new nanostructured membrane containing nanocellulose extracted from cotton linter and vitamin D₃ was produced using the “casting” technique. The membrane was characterized using high-resolution scanning electron microscopy (FEG-SEM) and Fourier transform infrared spectroscopy (FT-IR). The kinetics of vitamin release was quantified using molecular spectroscopy (UV-Vis).

Results

The FT-IR spectra showed the presence of all the active components in the membrane sample, without structural alterations or the formation of new bonds. The FEG-SEM images showed the presence of vitamin crystals on the surface and in the interior of the membrane. The release of vitamin D₃ occurred in a sustained manner, obtaining 3,029 IU mL^-1 of vitamin D₃ in 60 min.

Conclusions

The findings demonstrated that the membrane could be used for the sustained release of vitamin D₃. This new biomaterial has potential as a new model for vitamin supplementation in individuals with vitamin D₃ deficiency.

Drug release system

Cholecalciferol

Poly(vinyl alcohol)

Bioproducts

Biomaterial

Vitamin D₃ (cholecalciferol) is a steroid hormone produced by the photolytic action of UV-B radiation on the 7-dehydrocholesterol molecule found in the epithelial tissue, producing pre-vitamin D₃, which is absorbed and then metabolized in the liver to 25-hydroxyvitamin D₃. The latter subsequently reaches the renal tubules and is converted into the active form, 1,25-dihydroxyvitamin D₃ [1–3]. Evidence obtained in the laboratory indicates that the active hormonal form of vitamin D₃ induces a range of extra-skeletal biological responses, in addition to its effects in bone metabolism, including regulation of skin cell proliferation, effects in the cardiovascular system, and protection against various autoimmune diseases, multiple sclerosis, cancer, obesity, and inflammatory intestinal disease [4, 5].

More recently, vitamin D₃ has received increasing attention, because deficiency of this hormone has led to increasing cases of rickets, making it a global health issue. Epidemiological studies have found low levels of this vitamin throughout the global population, irrespective of age, level of education, or race [2, 6].

The three main factors involved in maintaining satisfactory levels of vitamin D₃ are adequate consumption of foods rich in cholecalciferol, sufficient exposure to solar UV-B to permit transdermal synthesis of the hormone, and dietary supplementation [5]. Populations that present inadequacy of one or more of these sources of vitamin D₃ are at risk of deficiency [7]. A difficulty is that oral ingestion methods can have limitations in individuals with intestinal disorders, Crohn’s disease, or chronic hepatic, gastric, and renal disorders. In these individuals, the intestinal absorption of this vitamin is compromised [8, 9].

The main risk factors for decreased serum levels of vitamin D₃ include excessive use of sunscreens, living in closed environments, chronic use of drugs that reduce serum cholesterol and consequently decrease levels of 7- dehydrocholesterol in the skin, advanced age, distance from the equator, air pollution, black skin, smoking, and intestinal malabsorption syndromes, among others [3, 5, 10].

Vitamin D₃ has low water solubility, so various formulations have been developed to increase its solubility, such as the use of liposomes, lipid emulsions, and mixed micelles [11–13]. However, these formulations are unstable, with degradation and oxidation easily occurring during storage. Therefore, further studies are needed of the incorporation of this hormone in new release systems [14, 15].

As an alternative to oral administration of vitamin D₃, which may present low efficiency due to the various barriers and different conditions encountered, application to the skin can offer advantages such as the treatment of psoriasis, atopic dermatitis, and other allergic skin diseases [16].

Materials based on nanocellulose (NC) have the potential for use in the sustained release of cosmetics and drugs [17, 18]. In addition to systemic effects, the local release of drugs using materials based on NC has also been explored [19]. The transdermal materials that have been developed, such as membranes, hydrogels, and microspheres, have been shown to enhance the efficacy of drugs, while reducing side effects [20, 21].

The market for nanocellulose was estimated to be worth US$250 million in 2019, while the number of scientific articles concerning the use of nanocellulose in medical and pharmaceutical applications has steadily increased over the last ten years [22]. However, there is still a vast field open for the development of new innovations, such as using different functionalities and interactions of bioactive agents, employing functional nanocelluloses for the development of new drug delivery systems and cell culture media, as well as for tissue engineering and for combating bacteria and viruses [23].

Meanwhile, the number of such products marketed in the medical and pharmaceutical areas remains low, which can be attributed to challenges such as the development of formulations incorporating highly lipophilic or high molar mass drugs, stability during storage, quality control of the drugs, and other factors such as limited production for large batches. It is expected that nanomaterials will find uses in cosmetic products such as lotions, soaps, and skin treatments, as has been discussed at conferences and can be seen in the patent literature. Cosmetics companies have shown interest in bio-based nanomaterials that are biocompatible and biodegradable [19].

Many investigations have already been undertaken by researchers and companies, with the aims of optimizing the production and marketing of nanocellulose. Nanocellulose has a wide range of applications in the health area, which could contribute to addressing the multiple challenges of modern society. It could have significant future contributions in materials engineering and the attainment of global sustainable development goals [24].

The aim of the present work was to develop an innovative system for the sustained release of vitamin D₃, employing a matrix of crystalline NC extracted from cotton linter. The development of a new nanostructured system for the sustained transdermal release of vitamin D₃ could enable convenient treatment of various skin conditions, as well as provide appropriate supplementation of this hormone.

The materials used for preparation of the membranes were as follows: Nanocellulose, obtained by acid hydrolysis of cellulose from cotton linter, polyvinyl alcohol (Dinâmica^®, batch 94332), vitamin D₃ (Fagron^®, sourced from Zhejiang Garden Biochemical High-Tech, batch C20170603A-5), liquid glycerin (Synth^®), Tween 80 surfactant (Dinâmica^®), and ultrapure water (resistivity > 18.3 MΩ.cm).

2.1. Nanocellulose extraction

The extraction of NC was performed by acid hydrolysis of cotton linter cellulose with sulfuric acid (64% w/w), using a ratio of 1:10 (pulp (g): acid (mL)), temperature of 60 ºC, and hydrolysis time of 30 min [25]. After this step, the solution obtained was dialyzed using a 21 mm diameter Servapor membrane. The size of the nanocellulose obtained was determined using high-resolution field emission gun scanning electron microscopy (FEG-SEM) [26].

2.2. Preparation of vitamin D₃ solution

The vitamin D₃ used in this work was supplied in the form of a whitish crystalline powder with a concentration of 40,000,000 IU g^-1. The amount of vitamin D₃ is usually presented using two units: i) micrograms (µg), or ii) international units (IU), where one microgram is equal to 40 IU [9]. Due to this high concentration, a 1:50 (w/w) pre-dilution was performed. A mass of 1.0 g of vitamin D₃ was weighed out and solubilized in 49.0 g of absolute ethyl alcohol, resulting in a concentration of 800,000 IU per gram of solution.

2.3. Synthesis of the membranes

Three types of membranes were produced (Table 1), two containing vitamin D₃ at different concentrations, and a control membrane without addition of the active agent. One membrane, denoted “nanovit”, was produced with a vitamin D₃ concentration of 8,000 IU mL^-1. A second membrane, denoted “nanovit super”, was produced with 40,000 IU mL^-1 of vitamin D₃. The control membrane was produced by adding glycerin, in the absence of the vitamin.

Table 1

Concentrations of vitamin D₃ in the membranes.
Membranes	Nanocellulose (mL)	PVA 5% (mL)	Glycerin (mL)	Tween 80 (mL)	Vitamin D₃ (IU mL^-1)
Nanovit	15	50	30	5	8,000
Nanovit super	15	50	30	5	40,000
Control	15	50	30	5	No addition

Liquid glycerin was used to solubilize the vitamin D₃ and as a plasticizer in the mixture, in order to promote membrane formation [27]. For synthesis of the membranes containing the active agent, two liquid glycerin solutions were prepared,

with different concentrations of the vitamin. For the first, 1.0 g of the solution of vitamin D₃ diluted 1:50 in absolute ethyl alcohol was weighed out and added to 30 mL of liquid glycerin, resulting in 800,000 IU of the vitamin in the solution. For the second solution, 5.0 g of the 1:50 solution of vitamin D₃ in absolute ethyl alcohol was used, resulting in 4,000,000 IU of the vitamin in the liquid glycerin solution.

The weighing employed an analytical balance with precision of 0.0001 g (model AW 220, Marte/Shimadzu^®).

As shown schematically in Fig. 1, the components were processed according to the “casting” technique, involving the preparation of a solution capable of forming membranes, following the methodology described by Fakhouri et al. [28].

A 15 mL volume of nanocellulose was added to 50 mL of 5% PVA solution, with sonication for 60 min to ensure complete dissolution of the nanocellulose, using an ultrasonicator (Ultronique^®, Ecosonics) with a 13 mm diameter titanium macrotip, operated at 500 W and frequency of 20 kHz. After these procedures, the PVA and NC solution was mixed with the glycerin solutions containing different concentrations of vitamin D₃. Tween 80 surfactant was then added to all the solutions, followed by sonication for 60 min.

The solutions were transferred to Petri dishes and left to evaporate at ambient temperature for 48 h, followed by heating in an oven for 24 h at 60 ºC.

2.4. Physical characterization of the membranes

The following parameters were considered in evaluation of the physical characteristics of the membranes: mass, thickness, diameter, elasticity, flexibility, tensile strength, and resistance to tearing and crushing [29–31]. The tests were performed in triplicate and the results were reported as means and standard deviations. The temperature during the tests was around 26 ºC.

The membranes were divided into two groups: i) control membranes, without addition of vitamin D₃, and ii) nanovit membranes, with addition of vitamin D₃.

Mechanical traction tests were performed using a Qualitylabor^® dynamometer to measure the stretching and strength of the membranes before and after incorporation of vitamin D₃ [32].

Cross-sectional area measurements were performed using membrane specimens with standardized dimensions of 60 mm (length), 20 mm (width), and 4 mm (thickness). These measurements were carried out following the dynamometer specifications for the test, using test specimens with standardized cross-sectional area of 80 mm² (width x thickness).

Tear resistance was measured using an Elmendorf model 53984 Digital Tear Tester (Frank^®), which uses a pendulum with defined loads to determine the force (kg.f) indicating the tear resistance of a material. Test specimens of the control and nanovit membranes were prepared with dimensions of 100 mm (length), 60 mm (width), and 4 mm (thickness) [33].

In the crushing test, the compression resistance (kN/m) of the membranes was measured using a Short Span Compression Tester (SCT, model 18510, Frank^®). The sample was placed in the open clamps, the device was switched on, and the clamps closed at a rate of 3 +/- 0.1 mm/min. The standardized specimen size was 60 mm (length), 20 mm (width), and 4 mm (thickness) [34].

2.5. Fourier transform infrared spectroscopy (FT-IR)

Spectra of the dried membrane samples were acquired using a Bruker Tensor 27 FT-IR instrument operated in fully attenuated reflection mode, in the range 350–4000 cm^-1, at a resolution of 4 cm^-1, with accumulation of 32 scans. The membranes were divided into two groups, without (controls) and with addition of vitamin D₃ (nanovit membranes) [35, 36].

2.6. High-resolution scanning electron microscopy (FEG-SEM)

A high-resolution field emission electron microscope (model JSM-7500F, JEOL) [26, 37, 38] was used to acquire images for evaluation of the control and nanovit membrane surfaces, the surface of the nanovit membrane after release of vitamin D₃ in absolute ethyl alcohol solution, and the size of the nanocellulose obtained from the cotton linter.

The samples were previously dehydrated, mounted on stubs, and metalized using a Bal-Tec^® SCD-50 system, followed by coating with carbon, under vacuum [39]. For all the carbon depositions, the distance from the sample was 50 mm and the vacuum was 2x10^-1 mbar. The presence of a conductive carbon layer is usually required to reduce the effect of electrical charge on the surface caused by the interaction between the electron beam and the sample, which can lead to distortion of the image [40].

2.7. Vitamin D₃ release kinetics

In the release assays, the quantification of vitamin D₃ released was performed by ultraviolet-visible (UV-Vis) spectrophotometry, using a SPECORD 50^® single-beam spectrophotometer fitted with deuterium (UV) and halogen (Vis) lamps, and a detector for measurement in the range from 190 to 1100 nm. The absorbance readings were obtained with the samples in 1 cm quartz cuvettes. Although the American Pharmacopoeia recommends the use of chromatographic methods for the direct quantification of drugs, spectrophotometric techniques have the advantages of being fast and relatively inexpensive. Hence, the use of spectrophotometry is an attractive option, given standardization of the methodology [41, 42].

The following procedures were performed in the release assays: i) selection of the wavelength at which vitamin D₃ presented the highest optical absorbance; ii) construction of the standard curve for calibration of the optical response of vitamin D₃, using serial dilutions in the linear range from 0 to 8,000 IU mL^-1, in absolute ethyl alcohol [43]; iii) selection of the receptor liquids for release of the vitamin, either a solution containing a mixture of 50% (v/v) absolute ethyl alcohol and 50% (v/v) alkaline phosphate buffer, or a solution of absolute ethyl alcohol alone; iv) recording of the absorbance peaks for the reagents used in the membrane procedures; and v) measurement of the release of the vitamin in the receptor liquid and construction of the curve of release as a function of time. The release assays were performed in beakers containing the membranes and 200 mL volumes of the receptor liquids, with 5 mL aliquots of solution being withdrawn after 5, 15, 30, 45, and 60 min, for analysis by UV-Vis spectrophotometry and determination of the concentration of vitamin D₃ released as a function of time [44].

3.1. Physical characterization of the membranes

Polymers, such as cellulose membranes, show behavior intermediate between elastic materials and high viscosity liquids [50]. The mechanical testing indicated that addition of the vitamin to the polymeric membrane matrix altered the resistance and specific deformation of the material. In principle, the addition of vitamin D₃ in the bulk membrane could lead to increases of cross-linking and interaction between the chains by van der Waals forces and hydrogen bridges, forming a three-dimensional network within the material [51].

The formation of crosslinks has a major influence on the mechanical properties of polymeric materials. In the case of sustained release techniques, it is essential that the mechanical characteristics of the materials are well known, since the strengths of the bonds between the components present in the membranes can influence processes such as those involved in the action of local drug release systems. Matrices with crosslinks between the polymer chains may enable membranes to absorb a greater quantity of water without dissolving, so their continuing adhesion to the surfaces on which they are applied can allow prolonged drug release over an extended period [52].

Evaluation was made of the general characteristics of the synthesized membranes. Figure 2 shows the physical aspects of the membranes, noting their elasticity and flexibility. It can be seen that the membranes were quite transparent, with an opalescent white color.

The results of the physical tests (Table 2) showed that the control and nanovit membranes had very similar physical characteristics, with no significant differences in terms of mass, thickness, and diameter. The nanovit membrane had a surface that was more oily, compared to the control membrane, which was due to the lipophilic nature of vitamin D₃.

Table 2

Physical characteristics of the control and nanovit membranes.
Membrane	Nanovit	Control
Mass (g)	12.095 ± 1.27	11.30 ± 1.25
Thickness (mm)	3.75 ± 0.35	3.55 ± 0.26
Diameter (mm)	93 ± 1.41	91.5 ± 1.29
Strength (N)	23.7 ± 2.68	13.9 ± 0.98
𝓁0 (mm)	60	60
Δ 𝓁 (mm)	40.8 ± 2.76	27.1 ± 1.87
𝓁 f (mm)	98.8 ± 2.77	85.7 ± 1.87
Area (mm²)	80	80
Strength (N/mm²)	0.29 ± 0.04	0.17 ± 0.17
Specific deformation (%)	64.7 ± 4.66	42.9 ± 3.18
Young’s modulus (MPa)	0.46 ± 0.02	0.40 ± 0.05
Crushing test (kN/m)	3.16 ± 0.10	3.02 ± 0.10
Tear resistance test (kg.f)	15.2 ± 0.66	11.5 ± 1.50

The data obtained in the traction tests indicated that the membrane chemical composition influenced the stretching behavior, with the presence of the vitamin acting to increase the elasticity. The nanovit membrane presented a stretching value of 40.8 mm, with a standard deviation of 2.76 mm, while the value for the control membrane was 27.1 mm, with a standard deviation of 1.87 mm.

The Young’s modulus parameter provides an indication of the intrinsic rigidity of a material [45, 46]. The nanovit membrane presented an average value of 0.46 MPa, with a standard deviation of 0.02 MPa, while the average value for the control membrane was 0.40 MPa, with a standard deviation of 0.05 MPa. The higher the Young’s modulus, the greater the resistance of the material to deformation [47]. Similar results were reported by Cinman (2014) and Drago (2014) for latex membranes impregnated with drugs, which presented Young’s modulus values of 0.86 and 0.63 MPa, respectively [48, 49].

Higher deformation percentage was observed for the membrane containing vitamin D₃ (average of 64.7%). When a stress was applied to the material, it became plastically deformed and did not return to its original shape.

3.2. FT-IR spectroscopic analysis

Analysis using FT-IR can enable the detection of possible changes in the physicochemical properties of the material and interactions among the components [53]. Hence, spectra were obtained for the nanocellulose, vitamin D₃, and the control and nanovit membranes.

The FT-IR spectrum for the nanocellulose showed two main absorption regions, with one in the low wavenumber region (800–1800 cm^-1) and the other at higher wavenumbers (2700–3800 cm^-1). A band at 1633 cm^-1 was characteristic of lignin and cellulose, attributed to the intramolecular hydrogen bonds of lignin, and vibrations of C-H and C-O in the cellulose polysaccharide rings. A band at 3286 cm^-1 corresponded to -OH stretching [54, 55].

The vitamin D₃ spectrum presented a band at 3317 cm^-1, attributed to -O-H hydrogen bonds, while bands at 2972 and 2891 cm^-1 corresponded to -C-H stretching. A band at 1377 cm^-1 corresponded to angular deformation of geminal dimethyl, while a band at 880 cm^-1 could be attributed to vibration of C = CH₂. The observed bands were in agreement with the literature [56, 57].

It could be seen from comparison of the FT-IR spectra for the control and nanovit membranes (Fig. 3) that there were no structural alterations of the membrane components. There was no appearance of new bands or other spectral alterations, indicating the absence of cross-linking or formation of new bonds in the membrane [58]. If such bonds were formed, they would be expected to influence the process of sustained release of vitamin D₃ [49].

The FT-IR results showed that all the components presented -OH stretching signals. The bands at 3286 and 1633 cm^-1 in the NC spectrum were also present in the spectra for the control and nanovit membranes. The bands at 1377, 1043, and 881 cm^-1 in the vitamin D₃ spectrum were also present in the nanovit membrane spectrum.

It should be stressed that no new bands were detected in the FT-IR spectra of the membranes, confirming the integrity of the drug in the membrane. Its functional groups were maintained, with no induction of cross-linking, indicating that this system possessed the necessary characteristics to function as a carrier for this active agent [59].

3.3. Characterization of the nanocellulose and the membranes by high-resolution electron microscopy (FEG-SEM)

The FEG-SEM images enabled visualization of the topography of the membranes, providing a detailed evaluation of the surface of the material and assessment of the dimensions of the NC used in the synthesis of the membranes.

The control membrane presented continuity, homogeneity, absence of porosity, and light surface texture (Figure 4(a)). There were no signs of aggregation of polymers visible on the membrane surface. This is a very important feature for this type of composite, since it maximizes the effect of incorporation of nanoparticles in the polymer matrix [60]. Similar results have been reported previously for electron microscopy analyses of nanocellulose membranes with characteristics analogous to those obtained here, in terms of topography [61–63].

Figure 4(b) shows a FEG-SEM image of the NC suspension obtained by acid hydrolysis of the cotton linter. It can be seen that at least one of the dimensions was at the nanometric scale, below 100 nm [64–66]. There was an entangled network of nanocrystals with a high degree of parallel orientation. The NC fibers presented tubular shapes, with widths in the region of 15 nm. It has been reported previously that cellulose nanocrystals obtained from the acid hydrolysis of fibers have the shape of small cylinders or rods [38, 67, 68].

Figure 4(c) shows the presence of micellar structures in the nanovit membrane, as expected since Tween 80 surfactant was added in the membrane synthesis, in order to reduce the surface tension between the components of the formulation [69]. This surfactant has the characteristic of forming micelles in mixtures of oil in water [70]. The image in Fig. 4(d), obtained at a magnification of 1,500x, shows the presence of many micelle-like structures. Figure 4(e) shows an image of a nanovit membrane after use in the assays of vitamin release in alcoholic solution. After release of the active agent, the membrane presented a porous, heterogeneous, and irregular surface. The presence of vitamin D₃ crystals can be seen within the bulk membrane. The structure of the membrane was altered, compared to the control membrane, suggesting that release of the drug occurred by a process of erosion, rather than by diffusion.

The image shown in Fig. 4(f), obtained at a magnification of 50,000x, shows the presence of crystals within the micelles, which could be attributed to vitamin D₃, since these structures were not observed in the control membrane. There was an absence of agglomerates on the membrane surface, providing further evidence that the NC and PVA matrix could be considered a satisfactory system for the release of vitamin D₃.

The characteristics of the control and nanovit membranes were consistent with those of polymeric materials with homogeneous topography, presenting absence of porosity, smoothness, continuity, and absence of agglomerates [71–73]. There was a clear difference in surface texture between the control and nanovit membranes, concerning surface oiliness and the presence of micelles. The FEG-SEM images showed the presence of vitamin D₃ crystals on the surface of the nanovit membrane, indicating that the system could provide rapid release of the drug.

3.4. Drug release assays

The release assays were performed using the control membrane (without addition of vitamin D₃), the nanovit membrane (with 8,000 IU mL^-1 of vitamin D₃), and the nanovit super membrane (with 40,000 IU mL^-1 of vitamin D₃).

The receptor liquid that showed the best optical response was absolute ethyl alcohol, since use of the solution with 50% ethyl alcohol and 50% alkaline phosphate buffer resulted in interference in the spectrophotometric reading, so it was not possible to perform UV-Vis analysis of these solutions [74–76]. After immersion of the membranes in 200 mL of absolute ethyl alcohol, under constant agitation, 5 mL aliquots were withdrawn at intervals of 60 min for measurement of absorbance [77].

Figure 5 shows the concentrations as a function of time, using the control membrane as a reference and obtaining the vitamin D₃ concentrations in the solutions exposed to the nanovit and nanovit super membranes. For the nanovit membrane, the drug reached the peak concentration rapidly, in 5 min, with a concentration of 250 IU mL^-1 in phase 1 of the graph. This behavior could be explained by the release of the drug adsorbed on the surface of the membrane, with the concentration values reaching a plateau in phase 2, due to the low concentration of vitamin D₃ [78, 79].

The nanovit super membrane presented 3 distinct phases of vitamin D₃ release. In phase 1, the release from the surface occurred in 5 min, reaching a concentration of 1,066 IU mL^-1. In phase 2, release between 5 and 30 min was related to the fraction of the drug present within the bulk membrane, obtaining values between 1,066 and 2,965 IU mL^-1. In phase 3, the release from the membrane reached a plateau, with a maximum value of 3,063 IU mL^-1 [80, 81].

It should be noted that the release from the nanovit membrane occurred rapidly, in 5 min, while the release from the nanovit super membrane lasted 30 min, until saturation of the receptor liquid.

Vitamin D₃ was successfully incorporated in a new system for drug delivery, formed by the association between crystalline nanocellulose, extracted from cotton linter, and polyvinyl alcohol. The “casting” technique used for membrane production was shown to be reproducible, facilitating the production of new membranes.

Analysis using FT-IR spectroscopy demonstrated that there was compatibility among the constituents of the membrane, without the formation of new chemical bonds. The FEG-SEM images showed that the material presented a homogeneous composition, highlighting the presence of drug crystals on the surface and within the membrane. The results confirmed that vitamin D₃ was incorporated in the membrane, with the interaction enabling fast and progressive release of the drug into the receptor liquid.

Further development of this system should consider the incorporation of other drugs, the use of the membrane in in vivo drug release studies, and the development of a quality control protocol to evaluate the stability of the membrane components.

Nanocellulose

PVA

Poly(vinyl alcohol)

FT-IR

Fourier transform infrared spectroscopy

UV-Vis

Molecular spectroscopy in the ultraviolet and visible regions

FEG-SEM

Field emission gun scanning electron microscopy

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

Not applicable.

CONSENT FOR PUBLICATION

Not applicable.

AVAILABILITY OF DATA AND MATERIALS

All data generated or analysed during this study are included in this published article.

COMPETING INTERESTS

All authors declare that they have no competing interests.

FUNDING

Not applicable.

AUTHORS' CONTRIBUTIONS

PLC and DG developed the original ideas of the study. PLC conducted most of the experiments. PLC and DG wrote the manuscript. DG helped to edit and revise the manuscript. All authors read and approved the final manuscript.

ACKNOWLEDGEMENTS

DG grant #2019/24684-2, São Paulo State Research Foundation (FAPESP).

AUTHORS' INFORMATION

a Department of Bioprocess and Biomaterial Engineering, Faculty of Pharmaceutical Sciences, State University of São Paulo (UNESP), Araraquara, SP, Brazil; b Itapeva Experimental Campus, State University of São Paulo (UNESP), Itapeva, SP, Brazil

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No competing interests reported.

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Controlled release of vitamin D 3 using a nanocellulose-based membrane

Archived Versions:

Version 1

Abstract

Background

Objective

Methods

Results

Conclusions

Figures

1. Introduction

2. Materials And Methods

2.1. Nanocellulose extraction

2.2. Preparation of vitamin D₃ solution

2.3. Synthesis of the membranes

2.4. Physical characterization of the membranes

2.5. Fourier transform infrared spectroscopy (FT-IR)

2.6. High-resolution scanning electron microscopy (FEG-SEM)

2.7. Vitamin D₃ release kinetics

3. Results And Discussion

3.1. Physical characterization of the membranes

3.2. FT-IR spectroscopic analysis

3.3. Characterization of the nanocellulose and the membranes by high-resolution electron microscopy (FEG-SEM)

3.4. Drug release assays

4. Conclusions

Abbreviations

Declarations

References

Additional Declarations

Supplementary Files

Archived Versions:

Version 1

Controlled release of vitamin D 3 using a nanocellulose-based membrane

Archived Versions:

Version 1

Abstract

Background

Objective

Methods

Results

Conclusions

Figures

1. Introduction

2. Materials And Methods

2.1. Nanocellulose extraction

2.2. Preparation of vitamin D3 solution

2.3. Synthesis of the membranes

2.4. Physical characterization of the membranes

2.5. Fourier transform infrared spectroscopy (FT-IR)

2.6. High-resolution scanning electron microscopy (FEG-SEM)

2.7. Vitamin D3 release kinetics

3. Results And Discussion

3.1. Physical characterization of the membranes

3.2. FT-IR spectroscopic analysis

3.3. Characterization of the nanocellulose and the membranes by high-resolution electron microscopy (FEG-SEM)

3.4. Drug release assays

4. Conclusions

Abbreviations

Declarations

References

Additional Declarations

Supplementary Files

Archived Versions:

Version 1

2.2. Preparation of vitamin D₃ solution

2.7. Vitamin D₃ release kinetics