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Research article
Qianfeng Xia
Hainan Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The emergence of antimicrobial resistance against mycobacterium tuberculosis has become the major concern in global tuberculosis control due to its limited therapy options and high mortality. However, the clinical and molecular characteristics of drug-resistant strains vary in different geographical areas. Hainan Island located in southern China, is a high drug-resistant tuberculosis burden area. This study aimed to determine the dynamic changes of drug-resistance patterns and drug-related gene mutation types of mycobacterium tuberculosis in Hainan from 2014 to 2019.
Results
A total of 1484 culture-confirmed mycobacterium tuberculosis were included in this study. It was found that the proportions of drug resistance to isoniazid and rifampin were 31.3% and 31.1% respectively. Overall, the proportion of multidrug resistant mycobacterium tuberculosis was 24.9%. Multivariate logistic regression analysis showed that age and the treatment history were independent influencing factors of drug resistant tuberculosis. The proportions of drug-resistant tuberculosis in retreatment patients were considerably higher than those in new patients. The most common mutation types of isoniazid were Ser315 → Thr (66.3%), and the most common mutation types of rifampin were Ser 531 → Leu (41.5%).
Conclusions
Our data suggests that the prevalence of drug resistant TB remains high in Hainan, and the risks for developing drug resistance with diversified and complicated mutation types increased significantly in retreatment patients. These results contribute to the knowledge of the prevalence of drug resistance in Hainan Province and also expand the molecular characteristics of drug resistance in China.
Keywords
Mycobacterium tuberculosis, CapitalBioTM DNA microarray, Drug sensitivity test, Molecular-characteristics, Drug-resistant rate
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CURRENT STATUS: Under Review
Version 1
Posted 25 Jan, 2021
No community comments so far
Editorial decision: Minor revision
On 21 Mar, 2021
Review #2 received
Received 19 Mar, 2021
Reviewer #2 agreed
On 07 Mar, 2021
Review #1 received
Received 06 Mar, 2021
Reviewer #1 agreed
On 04 Mar, 2021
Reviewers invited
Invitations sent on 03 Mar, 2021
Editor assigned
On 10 Jan, 2021
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On 10 Jan, 2021
Editor invited
On 10 Jan, 2021
First submitted
On 07 Jan, 2021
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This preprint is under consideration at BMC Microbiology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Qianfeng Xia
Hainan Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 25 Jan, 2021
No community comments so far
Editorial decision: Minor revision
On 21 Mar, 2021
Review #2 received
Received 19 Mar, 2021
Reviewer #2 agreed
On 07 Mar, 2021
Review #1 received
Received 06 Mar, 2021
Reviewer #1 agreed
On 04 Mar, 2021
Reviewers invited
Invitations sent on 03 Mar, 2021
Editor assigned
On 10 Jan, 2021
Submission checks complete
On 10 Jan, 2021
Editor invited
On 10 Jan, 2021
First submitted
On 07 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The emergence of antimicrobial resistance against mycobacterium tuberculosis has become the major concern in global tuberculosis control due to its limited therapy options and high mortality. However, the clinical and molecular characteristics of drug-resistant strains vary in different geographical areas. Hainan Island located in southern China, is a high drug-resistant tuberculosis burden area. This study aimed to determine the dynamic changes of drug-resistance patterns and drug-related gene mutation types of mycobacterium tuberculosis in Hainan from 2014 to 2019.
Results
A total of 1484 culture-confirmed mycobacterium tuberculosis were included in this study. It was found that the proportions of drug resistance to isoniazid and rifampin were 31.3% and 31.1% respectively. Overall, the proportion of multidrug resistant mycobacterium tuberculosis was 24.9%. Multivariate logistic regression analysis showed that age and the treatment history were independent influencing factors of drug resistant tuberculosis. The proportions of drug-resistant tuberculosis in retreatment patients were considerably higher than those in new patients. The most common mutation types of isoniazid were Ser315 → Thr (66.3%), and the most common mutation types of rifampin were Ser 531 → Leu (41.5%).
Conclusions
Our data suggests that the prevalence of drug resistant TB remains high in Hainan, and the risks for developing drug resistance with diversified and complicated mutation types increased significantly in retreatment patients. These results contribute to the knowledge of the prevalence of drug resistance in Hainan Province and also expand the molecular characteristics of drug resistance in China.
Figure 1
Figure 2
The full text of this article is available to read as a PDF.
This is a list of supplementary files associated with this preprint. Click to download.
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