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Research article
Michael J. Adang
University of Georgia
Corresponding Author
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Background Anopheles gambiae is the predominant vector of malaria, the fourth largest cause of infant mortality, in sub- Saharan Africa. Furthermore, A. gambiae is also the primary vector of O'nyong-nyong virus. The complexity of handling A. gambiae and infectious pathogens has led to the use of A. gambiae cell lines, including Ag55 cells, as a potential model to study vector-pathogen interactions and immune responses. The utility of cell lines can be maximized if their detailed gene expression profile and properties are available.
Results We provide detailed gene expression profiles, proteome and information about the properties of Ag55 cells. KEGG pathway analysis on genes with transcript levels of ≥200 FPKM revealed phagosome term enriched. Further, transcriptomic data backed by confocal microscopy and flow cytometry suggest that Ag55 cells have are hemocyte-like with phagocytic properties and immune competence.
Conclusion As Ag55 cells are immune competent and express hemocyte like properties they can be used as a model to study vector-pathogen immune response. Furthermore, the availability of transcriptomic data of Ag55 cells will help researchers use and engineer the Ag55 cell line in an efficient way, for example by developing strategies to make it more suitable for studies of interactions with Plasmodium and other microbes.
Keywords
Anopheles gambiae, next generation sequencing, flow-cytometry, phagocytosis, hemocyte-like, confocal microscopy, FPKM
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Michael J. Adang
University of Georgia
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 27 Feb, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background Anopheles gambiae is the predominant vector of malaria, the fourth largest cause of infant mortality, in sub- Saharan Africa. Furthermore, A. gambiae is also the primary vector of O'nyong-nyong virus. The complexity of handling A. gambiae and infectious pathogens has led to the use of A. gambiae cell lines, including Ag55 cells, as a potential model to study vector-pathogen interactions and immune responses. The utility of cell lines can be maximized if their detailed gene expression profile and properties are available.
Results We provide detailed gene expression profiles, proteome and information about the properties of Ag55 cells. KEGG pathway analysis on genes with transcript levels of ≥200 FPKM revealed phagosome term enriched. Further, transcriptomic data backed by confocal microscopy and flow cytometry suggest that Ag55 cells have are hemocyte-like with phagocytic properties and immune competence.
Conclusion As Ag55 cells are immune competent and express hemocyte like properties they can be used as a model to study vector-pathogen immune response. Furthermore, the availability of transcriptomic data of Ag55 cells will help researchers use and engineer the Ag55 cell line in an efficient way, for example by developing strategies to make it more suitable for studies of interactions with Plasmodium and other microbes.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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