Combining multiple anticancer agents in a nanocarrier can result in a formula with a low dose and few undesirable side effects.The purpose of the study was to formulate Garlic oil-loaded Mefloquine and Tamoxifen (TQ) in an oil-based nanoemulsion and evaluate its potential for inhibiting growth of lung cancer cells and normal skin cells.The antimalarial drug Mefloquine was repurposed using garlic oil nanoemulsion, which demonstrated better anti-cancer effects against A549 cell lines than Tamoxifen loaded nanoemulsion. The Mefloquine-loaded garlic nanoemulsion significantly reduced cell viability and promoted apoptosis in A549 cells in cytotoxic experiments. Physicochemical characterization, drug release tests, and cytotoxic studies were used to compare the drug-loaded nano emulsions. Mefloquine indicated less variance in hydrodynamic size, with a value of 1.01 ±0.13nm, than Tamoxifen-loaded nanoemulsion. Mefloquine loaded nanoemulsion showed better-sustained release, lower coarsening and constant colour stability. n simulated intestinal fluid, the drug release study of Mefloquine loaded nanoemulsion is 53.5% at 12 hours and Tamoxifen loaded nanoemulsion is 26.3 % at 8 hours (SIF). The percentage of cell viability of Mefloquine loaded Garlic oil nanoemulsion and Tamoxifen loaded Garlic oil nanoemulsion against lung cancer cells was 75.65 % and 64.35 %, respectively (A549). In normal cells, the cell viability of Mefloquine loaded Garlic oil nanoemulsion was lower than that of Tamoxifen loaded Garlic oil nanoemulsion. The findings imply that the Mefloquine-loaded Garlic oil nanoemulsion could be used as a nanotherapeutic carrier to target cancer cell without hampering the normal cells.