6-nitrodopamine (6-ND) is an endogenous modulator of the contractility in the rat isolated epididymal vas deferens (RIEVD) and considered to be the main peripheral mediator of the emission process. Use of selective and unselective b-adrenergic receptor antagonists have been associated with ejaculatory failure. Here, the effects of selective b1- and b1/ b2- adrenergic receptor antagonists on RIEVD contractions induced by 6-ND, dopamine, noradrenaline, adrenaline and EFS were investigated. The selective b1 adrenergic receptor antagonists atenolol (0.1 and 1mM), betaxolol (1mM) and metoprolol (1mM) and the unselective b1/b2- adrenergic receptor antagonists propranolol (1 and 10mM) and pindolol (10mM) caused significant rightward shifts of the concentration-response curve to 6-ND (pA2 6.41, 6.91, 6.75, 6.47 and 5.74; for atenolol, betaxolol, metoprolol, propranolol and pindolol), but had no effect on dopamine-, noradrenaline- and adrenaline-induced contractions. The effects of selective b1- and b1/ b2- adrenergic receptor antagonists at a higher concentration (atenolol 1mM, betaxolol 1mM, metoprolol 1mM, propranolol 10mM and pindolol 10mM) also reduced the EFS-induced RIEVD contractions in control but not in RIEVD obtained from L-NAME-treated animals. The selective b1- adrenoceptor agonist RO-363, the selective b2- adrenoceptor agonist salbutamol and the selective b3-adrenoceptor agonist mirabegron, up to 300 mM, had no effect on the RIEVD tone. The results demonstrate that b1- and b1-/ b2- adrenoceptor receptor antagonists act as 6-ND receptor antagonists in RIEVD, further confirming the main role of 6-ND in the RIEVD contractility.