Molecular characterization of a putative alphapartitivirus from Impatiens balsamina L.

doi:10.21203/rs.3.rs-1533276/v1

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Molecular characterization of a putative alphapartitivirus from Impatiens balsamina L.

https://doi.org/10.21203/rs.3.rs-1533276/v1

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Two double stranded RNAs (dsRNAs) that likely represent the genome of an alphapartitivirus tentatively named impatiens cryptic virus 1 (ICV1) were recovered from a symptomless Impatiens balsamina L.. DsRNA1 (2008 bp) codes for the RNA-dependent RNA polymerase (RdRp) of ICV1, which shares <83% amino acid sequence identities with the RdRp of other alphapartitiviruses. DsRNA2(1906 bp) codes for the capsid protein (CP) of ICV1, which shares <60% amino acid sequence identities with the CP of other alphapartitiviruses. Phylogenetic analysis suggested that ICV1 is closely related to plant alphapartitiviruses including vicia cryptic virus, beet cryptic virus 1, carrot cryptic virus and white clover cryptic virus 1. Reverse-transcription polymerase chain reaction with primers specific for dsRNA1 or dsRNA2 showed that ICV1 is common in I. balsamina from a wide range of places of China.

Partitiviridae

alphapartitivirus

impatiens cryptic virus 1

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Funding: This work was supported by the Science and Technology Project of Guizhou tobacco company（No. 201921) and the National Natural Science Foundation of China (No. 31972243). We are grateful to Dr. Fangluan Gao and Zhenguo Du from Fujian Agriculture and Forestry University for their help in preparing the manuscript.

Conflict of Interest: All authors declare that they have no conflict of interest.

Ethical approval: This article does not contain any studies with human participants or animals performed by any of the authors.

Vainio EJ, Chiba S, Ghabrial SA, Maiss E, Roossinck M, Sabanadzovic S, Suzuki N, Xie J, Nibert M (2018) ICTV Virus Taxonomy Profile: Partitiviridae. J Gen Virol 99(1):17–18& Ictv Report Consortium
Nibert ML, Ghabrial SA, Maiss E, Lesker T, Vainio EJ, Jiang D, Suzuki N (2014) Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research. Virus Res 188:128–141
Boccardo G, Lisa V, Luisoni E, Milne RG (1987) Cryptic plant viruses. Adv Virus Res 32:171–214
Boccardo G, Candresse T (2005) Complete sequence of the RNA2 of an isolate of white clover cryptic virus 1, type species of the genus Alphacryptovirus. Arch Virol 150(2):403–405
Suzuki A, Kobayashi F, Abe M, Uchiumi T, Higashi S (2001) Cloning and expression of a down-regulated gene (TrEnodDR1) of white clover responded by thenod genes derived from Rhizobium leguminosarum bv. trifolii strain 4S. 266:77–84Gene1
Nakatsukasa-Akune M, Yamashita K, Shimoda Y, Uchiumi T, Abe M, Aoki T, Kamizawa A, Ayabe S, Higashi S, Suzuki A (2005) Suppression of root nodule formation by artificial expression of the TrEnodDR1 (coat protein of white clover cryptic virus 1) gene in Lotus japonicus. Mol Plant Microbe Interact 18:1069–1080
Li H, Hu T, Fu J (2012) Identification of genes associated with adaptation to NaCl toxicity in perennial ryegrass (Lolium perenne L.). Ecotoxicol Environ Saf 79:153–162
Safari M, Ferrari MJ, Roossinck MJ (2019) Manipulation of aphid behavior by a persistent plant virus. J Virol 93(9):e01781–e01718
Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858
Guo L, Yang X, Wu W, Tan G, Fang S, Zhang S, Li F (2016) Identification and molecular characterization of Panax notoginseng virus A, which may represent an undescribed novel species of the genus Totivirus, family Totiviridae. Archives of virology, 161(3), 731–734
Blawid R, Stephan D, Maiss E (2007) Molecular characterization and detection of vicia cryptic virus in different Vicia faba cultivars. Arch Virol 152(8):1477–1488
Szegő A, Enünlü N, Deshmukh SD, Veliceasa D, Hunyadi-Gulyás É, Kühne T, Ilyés P, Potyondi L, Medzihradszky K, Lukács N (2010) The genome of beet cryptic virus 1 shows high homology to certain cryptoviruses present in phylogenetically distant hosts. Virus Genes 40(2):267–276
Willenborg J, Menzel W, Vetten HJ, Maiss E (2009) Molecular characterization of two alphacryptovirus dsRNAs isolated from Daucus carota. Arch Virol 154(3):541–543
Lu S, Wang J, Chitsaz F, Derbyshire MK, Geer RC, Gonzales NR, Gwadz M, Hurwitz DI, Marchler GH, Song JS, Thanki N, Yamashita RA, Yang M, Zhang D, Zheng C, Lanczycki CJ, Marchler-Bauer A (2020) CDD/SPARCLE: the conserved domain database in 2020. Nucleic Acids Res 48(D1):D265–D268
Nguyen LT, Schmidt HA, Von Haesele A, Quang MB (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular biology and evolution, 32(1), 268–274

renamede85b3.pdf
Full names of the viruses are presented in supplemental Table 1.

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Reviews received at journal
11 Apr, 2022
Reviewers invited by journal
11 Apr, 2022
Editor assigned by journal
09 Apr, 2022
First submitted to journal
07 Apr, 2022

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Molecular characterization of a putative alphapartitivirus from Impatiens balsamina L.

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