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Research article
Yu HUANG
Corresponding Author
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Background: Duck hepatitis A virus type 1 (DHAV-1) causes a highly contagious disease in domestic ducklings, which is traditionally characterized by lesions in the liver and rarely in the pancreas. However, several outbreaks of DHAV-1, which were characterized by yellow coloration and hemorrhage in pancreatic tissues, have occurred in China. The causative agent was named as pancreatitis-type DHAV-1. Genomic sequencing indicated variation rates of 3.4-6.5% in the genome of the pancreatitis-type DHAV-1 compared with those of the classical type DHAV-1. The antigenic relationship between the pancreatitis-type DHAV-1 and classical type DHAV-1 indicated large variation. However, the mechanism involved in infection of the pancreatitis-type DHAV-1 is still unclear.
Results: In the present study, transcriptome analysis of duck pancreas infected with classical type DHAV-1 and pancreatitis-type DHAV-1 was carried out. Following deep sequencing with Illumina-Solexa, a total of 53.9 Gb clean data were obtained from the cDNA library of the pancreas and a total of 29,597 unigenes with an average length of 993.43 bp were generated following de novo sequence assembly. The expression levels of D-3-phosphoglyceratedehydrogenase, phosphoserine aminotransferase, phosphoserine phosphatase, which are involved in glycine, serine and threonine metabolism pathway, were significantly downregulated in the pancreatitis-type DHAV-1-infected group compared with those of the classical type DHAV-1-infected group.
Conclusion: These findings provide information regarding differential expression levels of metabolism-associated genes between pancreatitis-type DHAV-1 and classical type DHAV-1, indicating that intensive metabolism disorders may contribute to different subtypes of DHAV-1-infection.
Keywords
Duck hepatitis A virus type 1, Duck pancreas, Metabolism disorder
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Yu HUANG
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 4
Posted 21 Jan, 2021
No community comments so far
No comments provided
No comments provided
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Duck hepatitis A virus type 1 (DHAV-1) causes a highly contagious disease in domestic ducklings, which is traditionally characterized by lesions in the liver and rarely in the pancreas. However, several outbreaks of DHAV-1, which were characterized by yellow coloration and hemorrhage in pancreatic tissues, have occurred in China. The causative agent was named as pancreatitis-type DHAV-1. Genomic sequencing indicated variation rates of 3.4-6.5% in the genome of the pancreatitis-type DHAV-1 compared with those of the classical type DHAV-1. The antigenic relationship between the pancreatitis-type DHAV-1 and classical type DHAV-1 indicated large variation. However, the mechanism involved in infection of the pancreatitis-type DHAV-1 is still unclear.
Results: In the present study, transcriptome analysis of duck pancreas infected with classical type DHAV-1 and pancreatitis-type DHAV-1 was carried out. Following deep sequencing with Illumina-Solexa, a total of 53.9 Gb clean data were obtained from the cDNA library of the pancreas and a total of 29,597 unigenes with an average length of 993.43 bp were generated following de novo sequence assembly. The expression levels of D-3-phosphoglyceratedehydrogenase, phosphoserine aminotransferase, phosphoserine phosphatase, which are involved in glycine, serine and threonine metabolism pathway, were significantly downregulated in the pancreatitis-type DHAV-1-infected group compared with those of the classical type DHAV-1-infected group.
Conclusion: These findings provide information regarding differential expression levels of metabolism-associated genes between pancreatitis-type DHAV-1 and classical type DHAV-1, indicating that intensive metabolism disorders may contribute to different subtypes of DHAV-1-infection.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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