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Case report
Yujian Liang
Sun Yat-Sen University
Corresponding Author
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Background: Pneumocystis jirovecii and Aspergillus fumigatus, are opportunistic pathogenic fungus that has a major impact on mortality in patients with systemic lupus erythematosus. With the potential to invade multiple organs, early and accurate diagnosis is essential to the survival of SLE patients, establishing an early diagnosis of the infection, especially coinfection by Pneumocystis jirovecii and Aspergillus fumigatus, still remains a great challenge.
Case presentation: In this case, we reported that the application of next
-generation sequencing in diagnosing Pneumocystis jirovecii and Aspergillus fumigatus coinfection in a Chinese girl with systemic lupus erythematosus (SLE). Voriconazole was used to treat pulmonary aspergillosis, besides sulfamethoxazole and trimethoprim (SMZ-TMP), and caspofungin acetate to treat Pneumocystis jirovecii infection for 6 days. On Day 10 of admission, her chest radiograph displayed obvious absorption of bilateral lung inflammation though the circumstance of repeated fever had not improved. Unfortunately, the patient discharged from the hospital since the financial burden, therefore further condition couldn't be tracked.
Conclusions: This successful application of the next generation sequencing assisting the rapid diagnosis of Pneumocystis jirovecii and Aspergillus fumigatus coinfection provides a new perspective in the clinical approach against the systematic fungi infections and highlights the potential of this technique in rapid etiological diagnosis.
Keywords
Pneumocystis jirovecii, Aspergillus fumigatus, Next generation sequencing, Case report
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CURRENT STATUS: Under Review
Version (no preprint)
Posted 19 May, 2021
Reviewer #1 agreed
On 19 May, 2021
Review #1 received
Received 19 May, 2021
Editor assigned
On 18 May, 2021
Reviewers invited
Invitations sent on 18 May, 2021
Submission checks complete
On 18 May, 2021
Editor invited
On 18 May, 2021
This version was not preprinted
Version 2
Posted 19 Mar, 2021
No community comments so far
Review #2 received
Received 02 May, 2021
Editorial decision: Minor revision
On 02 May, 2021
Review #1 received
Received 22 Mar, 2021
Reviewer #2 agreed
On 18 Mar, 2021
Editor assigned
On 11 Mar, 2021
Reviewers invited
Invitations sent on 11 Mar, 2021
Reviewer #1 agreed
On 11 Mar, 2021
Submission checks complete
On 11 Mar, 2021
Editor invited
On 11 Mar, 2021
First submitted
On 07 Mar, 2021
No comments provided
Editorial decision: Reject before peer review
On 11 Feb, 2021
Editor assigned
On 10 Feb, 2021
Editor invited
On 10 Feb, 2021
Submission checks complete
On 23 Jan, 2021
First submitted
On 21 Jan, 2021
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Subject Areas
Applied & Industrial Microbiology
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A new preprint design is coming!
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This preprint is under consideration at Italian Journal of Pediatrics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Case report
Yujian Liang
Sun Yat-Sen University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version (no preprint)
Posted 19 May, 2021
Reviewer #1 agreed
On 19 May, 2021
Review #1 received
Received 19 May, 2021
Editor assigned
On 18 May, 2021
Reviewers invited
Invitations sent on 18 May, 2021
Submission checks complete
On 18 May, 2021
Editor invited
On 18 May, 2021
This version was not preprinted
Version 2
Posted 19 Mar, 2021
No community comments so far
Review #2 received
Received 02 May, 2021
Editorial decision: Minor revision
On 02 May, 2021
Review #1 received
Received 22 Mar, 2021
Reviewer #2 agreed
On 18 Mar, 2021
Editor assigned
On 11 Mar, 2021
Reviewers invited
Invitations sent on 11 Mar, 2021
Reviewer #1 agreed
On 11 Mar, 2021
Submission checks complete
On 11 Mar, 2021
Editor invited
On 11 Mar, 2021
First submitted
On 07 Mar, 2021
No comments provided
Editorial decision: Reject before peer review
On 11 Feb, 2021
Editor assigned
On 10 Feb, 2021
Editor invited
On 10 Feb, 2021
Submission checks complete
On 23 Jan, 2021
First submitted
On 21 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Pneumocystis jirovecii and Aspergillus fumigatus, are opportunistic pathogenic fungus that has a major impact on mortality in patients with systemic lupus erythematosus. With the potential to invade multiple organs, early and accurate diagnosis is essential to the survival of SLE patients, establishing an early diagnosis of the infection, especially coinfection by Pneumocystis jirovecii and Aspergillus fumigatus, still remains a great challenge.
Case presentation: In this case, we reported that the application of next
-generation sequencing in diagnosing Pneumocystis jirovecii and Aspergillus fumigatus coinfection in a Chinese girl with systemic lupus erythematosus (SLE). Voriconazole was used to treat pulmonary aspergillosis, besides sulfamethoxazole and trimethoprim (SMZ-TMP), and caspofungin acetate to treat Pneumocystis jirovecii infection for 6 days. On Day 10 of admission, her chest radiograph displayed obvious absorption of bilateral lung inflammation though the circumstance of repeated fever had not improved. Unfortunately, the patient discharged from the hospital since the financial burden, therefore further condition couldn't be tracked.
Conclusions: This successful application of the next generation sequencing assisting the rapid diagnosis of Pneumocystis jirovecii and Aspergillus fumigatus coinfection provides a new perspective in the clinical approach against the systematic fungi infections and highlights the potential of this technique in rapid etiological diagnosis.
Figure 1
Figure 2
Figure 3
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