This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research Article
Jose-Luis Martinez-Guitarte
National University of Distance Education
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Plastic pollution is one of the leading environmental problems. Phthalates are widely used plastic additives released into the environment. Although the phthalates' effects have been extensively studied on vertebrates, there is a gap in knowledge on their effects on invertebrates. This work analyzes the impact of three phthalates, diethyl phthalate (DEP), benzyl butyl phthalate (BBP), and bis-(2-ethylhexyl) phthalate (DEHP), on the gastropod Physella acuta at the molecular level to establish the putative pathways involved in its response to them. By real-time PCR, we obtained the expression profile of thirty genes in one-week exposed animals at 0.1, 10, and 1000 µg/L. The genes cover the DNA repairing mechanism, detoxification mechanisms, apoptosis, oxidative and stress responses, immunity, energy reserves, and lipid transport. The results show that while DEP and DEHP did not cause alteration of the mRNA levels, BBP modulates almost all the genes tested. It can be concluded that the impact of BBP is extensive at the molecular level. However, it cannot be dismissed that the increase in transcriptional activity is a general response due to this compound’s well-known role as an endocrine disruptor. Additional research is needed to elucidate the differences observed in the impact of these compounds on this gastropod.
Keywords
Invertebrates, aquatic toxicology, plasticizers, gene expression array
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 01 Feb, 2021
No community comments so far
Editorial decision: Major revision
On 18 Mar, 2021
Reviews received
Received 14 Feb, 2021
Reviewers agreed
On 01 Feb, 2021
Reviewers invited
Invitations sent on 01 Feb, 2021
Editor assigned
On 01 Feb, 2021
Editor invited
On 28 Jan, 2021
Submission checks complete
On 28 Jan, 2021
First submitted
On 24 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Jose-Luis Martinez-Guitarte
National University of Distance Education
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 01 Feb, 2021
No community comments so far
Editorial decision: Major revision
On 18 Mar, 2021
Reviews received
Received 14 Feb, 2021
Reviewers agreed
On 01 Feb, 2021
Reviewers invited
Invitations sent on 01 Feb, 2021
Editor assigned
On 01 Feb, 2021
Editor invited
On 28 Jan, 2021
Submission checks complete
On 28 Jan, 2021
First submitted
On 24 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Plastic pollution is one of the leading environmental problems. Phthalates are widely used plastic additives released into the environment. Although the phthalates' effects have been extensively studied on vertebrates, there is a gap in knowledge on their effects on invertebrates. This work analyzes the impact of three phthalates, diethyl phthalate (DEP), benzyl butyl phthalate (BBP), and bis-(2-ethylhexyl) phthalate (DEHP), on the gastropod Physella acuta at the molecular level to establish the putative pathways involved in its response to them. By real-time PCR, we obtained the expression profile of thirty genes in one-week exposed animals at 0.1, 10, and 1000 µg/L. The genes cover the DNA repairing mechanism, detoxification mechanisms, apoptosis, oxidative and stress responses, immunity, energy reserves, and lipid transport. The results show that while DEP and DEHP did not cause alteration of the mRNA levels, BBP modulates almost all the genes tested. It can be concluded that the impact of BBP is extensive at the molecular level. However, it cannot be dismissed that the increase in transcriptional activity is a general response due to this compound’s well-known role as an endocrine disruptor. Additional research is needed to elucidate the differences observed in the impact of these compounds on this gastropod.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
Loading...