The Phytochemical analysis, Metabolic profiling, Anti-bacterial and Anti-oxidant activity of Nepeta cataria

DOI: https://doi.org/10.21203/rs.3.rs-154568/v1

Abstract

Introduction: Nepeta cataria is a naturally growing plant in the regions of Pakistan, in English name is Catnip mint and the local name is Badranj boya. This plant has immense pharmacological and medical importance. It is used in herbal and traditional medicine as an antipathogenic agent. However detailed phytochemical analyses in a wide spectrum of solvents and their antimicrobial analysis against both Gram-positive and negative limited studied.

Objectives: This study aimed to identify phytochemicals, metabolome, antimicrobial potential, and antioxidant analysis of Nepeta cataria (Pakistani plant).

Methods: Plant collection, identification, cleaning, and grinding were performed followed by extract preparation in five solvents i.e. methanol, ethanol, water, acetone, and hexane. Qualitative phytochemical analysis was performed by general biochemical tests. . GC/MS was used to identify metabolic compounds in all extracts. Antibacterial analysis for both gram-positive and negative bacteria was performed by Kirby’s Disc method, 96 well test, and Resazurin test. The antioxidant activity was determined by DPPH assay.

Results: Kirby disk diffusion method results indicated that Nepeta cataria extracts in different solvents maximum inhibition percentage against tested bacteria was 250-1000 µg/ml. Resazurin method inhibition against bacterial strains was 6.25-100 µl/ml. The Phytochemical analysis for Phenolic Compounds, Flavonoids, Terpenoids, Cardiac Glycosides, Free Anthraquinones, Combined Anthraquinones, Tannins, Alkaloids, and DPPH was positive in the different extracts. The ORAC was 133759.021um/100ml in concentration. GC/MS analysis of methanolic extract (71 identified + 48 unknown),ethanol-based extract ( 80 known + 31unknown), water-based extract ( 28 known + 11 unknown ), acetone-based extract ( 13 known + 9 unknown) , hexane based extract (11 known + 8 unmatched) phytochemicals were detected.

Conclusion: Current study concludes the remarkable antibacterial and antioxidant potential of Nepeta cataria extracts.

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