A total of 39 sequences consisting of 679 characters were included in the phylogenetic analysis. The alignment includes 37 sequences of A. mougeotii, E. deutziae, E. deutziicola, and some Type sequences of Golovinomyces spp., as well as two outgroup sequences. As shown in Fig. 1, the desired sequence placed in Arthrocladiella clade, and forms a sister clade with Golovinomyces spp. This sequence showed one base substitution when compared with other sequences of A. mougeotti retrieved from GenBank.
Mycelium amphigenous, hyphal appressoria nipple-shaped, conidiophores arising from upper surface of the mother cell, erect, 60–130 × 9–11 µm, sometimes there is a concavity below the mother cell, foot-cells straight to slightly sinuous, 28–44 × 8–13 µm, followed by 1–4 (5) shorter cells (mostly 3), forming conidia in chain, conidia ellipsoid, obovoid, cylindrical, 28–37 × 10–16 µm, conidial germination ± terminal, from one or both ends, sometimes two sides of one end (Fig. 3).
We found some immature chasmothecia on examined specimens. However, there is a brief description of the sexual state of this specimen in Iran (Abbasi et al 2013): It seems these authors found more chasmothcia and used them for their examination. They have described the sexual state for this specimen as follows: chasmothecia were 100–160 µm diam., asci often obovoid or rather clavate, 50–56 × 22–26 µm, the appendages mostly tend to point to one direction, dichotomously or trichotomously branched, primary branches deeply divided and the following branchlets are shorter. Apex of the appendages are straight. According to above mentioned features it seems they observed some morphological characteristics fits well with A. mougeotii especially the features related to the appendages.
After fungal identification and having molecular support for A. mougeotii, we re-examined the host plant specimen (Fig. 2) using JSTOR Global Plants database, and compared Iranian specimen with type material of Deutzia gracilis (L0176009) and two authentic specimens of Lycium europaeum (MA108036 & B -W 04474 -01 0). The above comparison also confirmed the fungus ID and showed that host plants is definitely belong to the genus Lycium.
Specimen examined: onLycium sp., J. Boujari, 3/Dec/2012, Tehran, Iran (IRAN 16117 F)
Based on host plant and primary investigations, this specimen was regarded as a member of Erysiphe deutziae species complex, but current molecular studies shed light on the mysterious state of this specimen. The genus Arthrocladiella is reported as a new genus for the Mycobiota of Iran.