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Research
Jian Li
the Third Hospital of Mianyan
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5807-2360
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Claudin 18 (CLDN18) is a transmembrane protein localized in the apical regions to form a tight-junction complex. The expression of CLDN18 was restricted to normal lung and stomach tissues but was aberrantly activated in some types of cancers, and this molecule can serve as a promising target for targeted treatment.
Methods
The mRNA expression, genetic variations and prognostic values of CLDN18 in pancreatic cancer (PC) were analyzed using public data from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), Human Protein Atlas (HPA) databases and multiple online tools. The role of cyclic AMP-responsive element binding protein (CREB) and DNA methylation in regulating the expression of CLDN18 was determined by correlation analysis.
Results
The expression of CLDN18 was restricted to the lung and stomach in normal tissues, but was ectopically overexpressed in PC. CLDN18 mRNA expression was higher in cancers with advanced local invasion, later clinical stage, infiltrating duct or mucinous carcinoma types. Genetic variations of CLDN18 are rarely observed in PC. Correlation analysis revealed that CREB3L1, CREB3L3, and CREB3L4 were significantly positively coexpressed with CLDN18, and CLDN18 expression increased as methylation gradually decreased. The expression of CLDN18 was not observed to plays a role in predicting the survival of PC patients.
Conclusions
This data-driven study summarizes the expression features of CLDN18 in PC and demonstrates that it may serve as an ideal biomarker and therapy target for PC.
Keywords
CLDN18, Pancreatic cancer, Gene expression, Bioinformatic analysis, Prognosis
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This is a preprint. It has not completed peer review.
Research
Jian Li
the Third Hospital of Mianyan
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5807-2360
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 28 Feb, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Claudin 18 (CLDN18) is a transmembrane protein localized in the apical regions to form a tight-junction complex. The expression of CLDN18 was restricted to normal lung and stomach tissues but was aberrantly activated in some types of cancers, and this molecule can serve as a promising target for targeted treatment.
Methods
The mRNA expression, genetic variations and prognostic values of CLDN18 in pancreatic cancer (PC) were analyzed using public data from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), Human Protein Atlas (HPA) databases and multiple online tools. The role of cyclic AMP-responsive element binding protein (CREB) and DNA methylation in regulating the expression of CLDN18 was determined by correlation analysis.
Results
The expression of CLDN18 was restricted to the lung and stomach in normal tissues, but was ectopically overexpressed in PC. CLDN18 mRNA expression was higher in cancers with advanced local invasion, later clinical stage, infiltrating duct or mucinous carcinoma types. Genetic variations of CLDN18 are rarely observed in PC. Correlation analysis revealed that CREB3L1, CREB3L3, and CREB3L4 were significantly positively coexpressed with CLDN18, and CLDN18 expression increased as methylation gradually decreased. The expression of CLDN18 was not observed to plays a role in predicting the survival of PC patients.
Conclusions
This data-driven study summarizes the expression features of CLDN18 in PC and demonstrates that it may serve as an ideal biomarker and therapy target for PC.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
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