The Plasmonic surface plasmon resonance (SPR) device was used to develop a rapid, simple, and specific immunoassay to detect adulteration in milk. The rapid detection of adulteration such as Urea, Melamine, and Salmonella is a major concern for the food industry. In the present study, urea, melamine, and salmonella are detected in cow and buffalo milk using SPR based technique. The performance of the proposed sensor for urea detection in buffalo and cow milk is 37.952°/RIU and 38.44°/RIU, respectively. In contrast, the sensitivity detected by the proposed SPR sensor for the salmonella and melamine salmonella adulteration in the milk is 22.0025°/RIU and 179.125°/RIU, respectively.