Complete genome analysis of a novel chuvirus from a southern green stink bug, Nezara viridula

doi:10.21203/rs.3.rs-1557313/v1

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Research Article

Complete genome analysis of a novel chuvirus from a southern green stink bug, Nezara viridula

https://doi.org/10.21203/rs.3.rs-1557313/v1

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A novel chuvirus from the southern green stink bug (Nezara viridula) was identified by RNA sequencing in this study, which was named "Nbu southern green stink bug chuvirus-1" (NbuSGSBV-1). The complete genome sequence of NbuSGSBV-1 consisted of 11,375 nucleotides, which was confirmed to be a circular form by ‘around-the-genome’ reverse transcription polymerase chain reaction (RT-PCR) and sanger sequencing. Furthermore, three open reading frames (ORFs) were predicted in the NbuSGSBV-1 genome, including a large polymerase protein (L protein), a glycoprotein (G protein) and a nucleocapsid protein (N protein). Thereafter, a phylogenetic tree was constructed based on the RNA-dependent RNA polymerase amino acid sequences of all the currently available viruses in the family Chuviridae. As a result, NbuSGSBV-1 was clustered together with Sanya chuvirus 2 and Hubei odonate virus 11, indicating that NbuSGSBV-1 might belong to the genus Odonatavirus. Meanwhile, motif prediction results revealed five conserved sites among the L proteins of NbuSGSBV-1 and its homologous chiviruses. Moreover, the high abundance and typical characteristics of the NbuSGSBV-1-derived small interfering RNAs suggested the active replication of NbuSGSBV-1 in the host insect. To the best of our knowledge, this is the first report of a chuvirus identified from the insect family Pentatomidae. The discovery and characterization of NbuSGSBV-1 will help to understand the diversity of chuviruses in insects.

Nbu southern green stink bug chuvirus-1

Chuviridae

Virus discovery

Nezara viridula

Acknowledgements

This work was supported by the National Natural Science Foundation of China (U20A2036) and Ningbo Science and Technology Innovation 2025 Major Project (2019B10004).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical statement

No experimental work with humans was done in this study.

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fig.s1.tif
Supplementary Figure S1: Sanger sequencing for the conjunction region of 5′ and 3′ terminus of NbuSGSBV-1.
fig.s2.tif
Supplementary Figure S2: Multiple sequence alignments and motif locations of L protein from NbuSGSBV-1 and closely related chuviruses. Five conserved motifs were identified using MEME, And the conserved sites are marked with red boxes.
Supp.file.s1.COIsequenceoftheNezaraviridula.fasta
Supplementary File S1: The COI sequence of Nezara viridula.
Supp.file.s2.FullgenomesequenceofNbuGSBV1.fasta
Supplementary File S2: The full genome sequence of NbuSGSBV-1.
SupplementaryTableS1.BLASTresultsofNbuGSBV1.docx
Supplementary Table S1: The BLAST results of NbuSGSBV-1 compared with the NCBI NT and NR databases.
SupplementaryTableS2.Primersusedinthisstudy.docx
Supplementary Table S2: Primers used in this study.

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Reviews received at journal
02 May, 2022
Reviewers invited by journal
02 May, 2022
Editor assigned by journal
14 Apr, 2022
First submitted to journal
14 Apr, 2022

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Complete genome analysis of a novel chuvirus from a southern green stink bug, Nezara viridula

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