A Nomogram for Predicting Recurrence-free Survival of intermediate and High risk Neuroblastoma

DOI: https://doi.org/10.21203/rs.3.rs-1587209/v1

Abstract

Purpose

This study aimed to confirm the independent risk factors for recurrence-free survival (RFS) in intermediate and high-risk neuroblastoma (NB) patients, and set up an effective Nomogram model for predicting the recurrence of NB.

Methods

A total of 212 children with intermediate- and high-risk neuroblastoma, who had ever achieved complete remission (CR) or very good partial remission (VGPR) after standardized treatment in this hospital, were chosen as study objects. After retrospective analysis of the clinical data, Cox regression model was used to explore the factors related to the recurrence of neuroblastoma, to determine the variables to construct the Nomogram. The consistency index would predict the accuracy of this Nomogram.

Results

RFS rate in 1-year, 3-year, 5-year, and 10-year was 0.811, 0.662, 0.639, and 0.604, respectively. Children with MYCN amplification had a higher Neuron-specific Enolase (NSE) value (P = 0.031) at the initial diagnosis than MYCN non-amplification. The univariate analysis predicted that increased Vanilmandelic Acid (VMA) and NSE value, and Dehydrogenase (LDH) > 1000U/L were important adverse factors for the recurrence of NB. Multivariate analysis demonstrated that age at diagnosis, tumor localization, MYCN state, histologic subtype, and tumor capsule were significantly associated with RFS (all P values < 0.05). Nomograms were established for predicting the recurrence of NB according to the Cox regression analysis. Internal verification by the Bootstrap method showed that the prediction of the nomogram's consistency index (C-index) was 0.824 (P = 0.023).

Conclusion

Age at diagnosis, tumor localization, MYCN state, histologic category, and tumor capsule were independent risk factors for the recurrence of NB. The Nomogram model could accurately predict the recurrence of children with neuroblastoma.

Introduction

Neuroblastoma (NB) sprang from the multipotent neural crest cells in the sympathetic nervous system. It preferably occurred in the adrenal medulla. It could also be extended to the paravertebral sympathetic nerves of the neck, chest, abdomen, and pelvis(1). NB was the most typical extracranial solid tumor in childhood, with an incidence rate of about 8–10% of all childhood malignant tumors. Nevertheless, mortality accounted for 15% of childhood malignant tumor-related deaths (2–4). Due to the insidious onset of NB, about 40% of children had bone marrow, bone, or other metastases at the first diagnosis, leading to a poor general prognosis (5). Their prognoses could vary greatly due to the high heterogeneity. The 5-year survival rate of low-risk NB exceeded 90%, while the 5-year survival rate of children in the intermediate and high-risk groups was not satisfactory (6). It was still difficult to cure through comprehensive multidisciplinary treatment such as surgery, chemotherapy, and radiotherapy. Recurrence often occurred after complete remission (CR), and the survival rate after recurrence was less than 20% (2, 7). It was crucial to precisely determine the recurrence by monitoring NB-related indicators and to give timely salvage treatment, especially in the intermediate and high-risk groups.

Clinically, we defined relapse-free survival (RFS) as the duration from CR or very good partial response (VGPR) to recurrence. Chinese Children’s Cancer Group (CCCG) of the China Anti-Cancer Association developed the Multidisciplinary Treatment Guideline for Neuroblastoma. It defined CR that all primary and metastatic tumors disappeared and the value of catecholamines and metabolites decreased to standard Level. It defined VGPR that the primary tumor volume decreased by 90–99%; all measurable metastases disappeared; catecholamines and metabolites got back to normal. VGPR allowed a 99Tc scan of bone lesions to be positive (because bone metastases did not heal), but if (123I)-MIBG was performed, all lesions would be negative(8). Studies had shown that the (123I)-MIBG scan could effectively predict RFS in high-risk NB patients, but the related factors affecting RFS needed further study (9). Many studies reported that the prognostic factors for NB include age, pathological type, International Neuroblastoma Staging System (INSS), MYCN state, etc. (10, 11). It also reported that the time from diagnosis to the first recurrence could affect survival in recurrent NB patients (7). The survival rate of recurrent NB was relatively low, so it was significant to monitor recurrence accurately. Therefore, the study was performed to analyze: (a) the clinical features of patients with recurrent NB; (b) the relevant risk factors for RFS and set up a Nomogram model to predict RFS. It could accurately predict the risk of recurrence of NB for children who had reached CR or VGPR and guide the follow-up interval after CR or VGPR in this center.

Medical Nomograms used clinic data and other variables, such as tumor stage and age, to set up a statistical prediction model. It could predict the probability of recurrence or death for a specific individual (12). The most significant advantage was that it could assess the risk of clinical events individually according to patient and disease characteristics. The Nomogram could incorporate continuous variables and independent risk factors for the disease into the prognosis. It was superior to the clinician in assessing events and had a wide range of applications in various cancers (13–15). Therefore, we established a predicting nomogram based on the relevant factors affecting the RFS of recurrent NB, which could help clinicians plan the follow-up interval and detect recurrence in time.

Materials And Methods

2.1.1 Patients

There were 212 cases of pediatric NB enrolled in this study from January 1, 2007, to December 31, 2018. They were all treated by the Multidisciplinary Treatment Guideline for Neuroblastoma in the Department of Pediatric Hematology/Oncology, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine. The inclusion criteria for patients were indicated as below, (a) risk groups were the intermediate-risk group or high-risk group according to Children's Oncology Group (COG). (b) patients had achieved CR or VGPR after standardized treatment according to CCCG’S guidelines. (c) patients were younger than 18 years old at diagnosis. We retrospectively collected clinical characteristics of each patient concerning age, gender, chief complaint, INSS stage, primary site, metastasis site (especially bone and bone marrow metastasis), MYCN state (amplified or not), histologic category, intact tumor capsule (with, without), tumor size (5cm or less, 5.1-9.9cm, 10cm or more), the time-point for reaching CR or VGPR, etc. The value of Vanilmandelic Acid (VMA), Neuron-specific Enolase (NSE), serum ferritin, and Lactic Dehydrogenase (LDH) before treatment also were recorded. The tumor capsule and tumor size were recorded according to the surgeon's exploration and measurement during the operation. For the recurrent children, we paid more attention to identifying the recurrent time to determine RFS.

2.1.2 Diagnosis, pathology, stage, and risk stratification

Two methods were used to diagnose neuroblastoma in this center. (a) Pathological diagnostic criteria were the gold standard for NB. (b) Imaging, bone marrow puncture or biopsy suggested suspicious NB, accompanied by a significant increase in urine (or serum) catecholamines or their metabolites. International Neuroblastoma Pathology Classification (INPC) was adopted for the histologic category of neuroblastoma. It included NB(undifferentiated or poorly undifferentiated ), NB༈differentiated ), Ganglioneuroblastoma intermixed (GNBi), and Ganglioneuroblastoma nodular (GNBn)(16, 17). Patients were classified by INSS. It defined Stage 1, 2A, or 2B, and 4s as the localized tumor, Stage 3 as an unresectable infiltrating tumor, and Stage 4 as any primary tumor with dissemination. Finally, patients were divided into the low-risk group, intermediate-risk group, and high-risk group respectively according to age, INSS, INPC, MYCN state, etc, by COG criteria.

2.1.3 Treatment Procedure

Regarding the CCCG’s guidelines, children in the intermediate-risk group would be treated with alternative surgery before chemotherapy or after (about four cycles), then following chemotherapy till the end of four cycles after CR or VGPR. The total cycles of chemotherapy did not exceed eight. A second surgery could be operated on if necessary. The maintenance treatment was 13-cis-RA 160mg/m2/d, consecutive 14 days per month, six months in total. While cases of the high-risk group were treated with neoadjuvant chemotherapy (about four cycles) followed by selective operation, then additional four cycles of chemotherapy after CR or VGPR. This group should be taken tandem autologous stem cells transplantation and local radiotherapy after conventional chemotherapy. But patients who could not afford stem cell transplantation should take 10 to 12 cycles of chemotherapy in total. The maintenance treatment was the same as the intermediate-risk group.

2.1.4 Follow-up of Patients

Each patient had a unique portfolio that recorded their treatment response, treatment effect, and follow-up. We looked through their portfolio to find out when they achieved CR or VGPR. If the portfolio was incomplete or the patient did not follow up on schedule, we would call them to complete a brief oral questionnaire through the telephone. At first, all patients were followed up with lab examinations (VMA and NSE) and Imaging examinations (CT, MRI, or PET/CT) every two cycles of chemotherapy. After CR or VGPR, they were followed up every three to six months. Event was defined as recurrence or death for any cause.

2.1.5 Statistical analysis

All statistical analysis was performed using SPSS statistical software for Windows, version19.0 (SPSS, Chicago, IL, USA). The quantitative data were expressed as median (range). The value of VMA, NSE, serum ferritin, and LDH satisfied the non-normal distribution. Wilcoxon rank-sum test was used to show the correlation between MYCN amplification and clinical features of neuroblastoma. The count data were expressed by proportion (%). RFS was analyzed through Kaplan–Meier curves, and the Log-rank test was used for univariate survival analysis to determine clinical factors related to recurrence. Cox proportional hazards model was applied to multivariate analysis to evaluate independent risk factors affecting recurrence. Finally, those risk factors that had been demonstrated statistically significant in univariate survival analysis would be built into a predictive recurrence Nomogram. All tests were bilateral, and p-values less than 0.05 were regarded as statistical significance(18).

The nomogram was derived from the results of Cox regression analysis and compiled using the RMS package in R version 3.6.2 (http://www.R-project.org/). One thousand re-sampling Bootstraps were performed for internal verification to reduce over-fitting bias. The consistency index (C-index) was used to evaluate the predictive performance of the nomogram. It represented the probability of consistency between actual recurrence and recurrence predicted from the model. The C-index ranged from 0 to 1. The prediction would be more accurate if the C-index were close to one(19).

Results

2.2.1 Demographics

In this cohort, there were 131 boys (61.8%) and 81 girls (38.2%), and the male-to-female ratio was 1.61:1. Sixty-three children (29.7%) were less than 18 months old, and 249 children (70.3%) were 18 months and above. The median age at initial diagnosis was 32 months (range from 1.2 to 156.3 months). Fifty-seven cases (26.9%) were found in the physical examination without any symptoms. Forty-nine cases (23.1%) were palpated by their parents. The chief complaint of 59 cases (27.8%) was digestive system symptoms such as jaundice, vomiting, and diarrhea. The initial symptom of 21 cases (9.9%) was asthenia, muscle strength and muscle tension changes, or other motor symptoms. Cough, hemoptysis, dyspnea, and other respiratory symptoms were chief complaints in 10 patients (4.7%). Fourteen patients (6.6%) only presented with fever (6.6%). MYCN genetic testing had been carried out since 2012 in this center, so 170 out of 212 patients had been detected, including 125 (58.9%) non-amplified patients and 45 (21.2%) amplified patients. RFS rate in 1-year, 3-year, 5-year, and 10-year was 0.811, 0.662, 0.639, and 0.604, respectively. The demographic and clinical characteristics of 212 NB patients were listed in Table 1.

The primary sites of neuroblastoma were mainly retroperitoneal/paranephros (166 cases, 78.3%) and mediastinum (33 cases,15.6%). Bone and bone marrow were the main metastasis sites. There were 69 patients (32.5%) with bone and bone marrow metastasis simultaneously. Eighty-nine children (42%) had bone metastases, including 10 cases (4.7%) with skull metastases, 20 cases (9.4%) with limb bone metastases, 17 cases (8%) with trunk bones metastases, and 42 cases (19.8%) with multiple bone metastases.

Eighty-two children (38.7%) had bone marrow metastasis at the initial onset. They accepted bone marrow biopsy before the therapy. The minimal residual disease (MRD) in their bone marrow was positive (MRD%≥0.01%) through flow cytometers. So, they were asked to have a bone marrow puncture to monitor MRD every two cycles of chemotherapy. After two cycles, MRD turned negative (<0.01%) in 54 cases (25.5%), while MRD in 24 cases (11.3%) and 4 patients (1.9%) converted negative after four and six cycles, respectively. Univariate analysis of RFS was performed on the above three groups of patients (2, 4, and 6 cycles). The median RFS of the three groups was 25.75 months, 12.07 months, and 39.80 months, respectively (P=0.023). Given the small number of patients whose MRD turned negative after 6 cycles of chemotherapy, the above comparison might be biased, so we compared the RFS of patients with negative MRD after 2 and 4 cycles by the Kaplan-Meier curve. (Figure 1).

2.2.2 Recurrent neuroblastoma

Seventy-one patients (33.5%) developed recurrent NB including 47 boys (66.2%) and 24 girls (33.8%). The ratio of male-to-female was 1.95:1. The median interval of recurrence from CR or VGPR was 10.27 months (range from 1.53 to 64.83months). While the median follow-up time was 70.80 months (range from 13.30 to 161.06months). Forty cases (56%) relapsed within one year. Sixty-two cases (87.3%) were older than 18 months at the initial diagnosis. Two cases (2.8%) were in stage INSS 2; 9 cases (12.7%) were in INSS stage 3; while 60 patients (84.5%) were in INSS stage 4. Eight (11.2%) were divided into the intermediate-risk group, while 63 cases (88.8%) were divided into the high-risk group (COG). The pathological type of 40 patients was undifferentiated neuroblastoma. Forty-six (64.8%) of them had bone marrow metastases.

2.2.3 Univariate analysis of RFS

The Kaplan-Meier curve was used to conduct a univariate analysis of the correlation between clinical data and recurrent NB. The univariate analysis was performed on the relevant laboratory tests of the newly diagnosed children. Children with increased NSE values, LDH>1000U/L, and increased VMA values had lower RFS (P=0.048, P=0.002, P<0.001, respectively, Log-rank test, figures 2A, B, C). Children with MYCN amplification also had lower RFS (median: 34.03 months vs. 42.23 months, P = 0.017, Log-rank test, figure 2D). Age and RFS showed a negative correlation. The risk of recurrence was increased in older children (median:62.30 months vs. 59.77 months vs. 36.33months, P corrected=0.0002, Log-rank test, figure 2E). For the pathological subtypes, the median RFS was the longest (57.47 months) in children with GNBi and the shortest (32.60 months) in children with GNBn (P = 0.023, Log-rank test, figure 2F). RFS was correlated with tumor localization. The median RFS of stages 1, 2, and 4s was 90.67 months, the median RFS of stage 3 was 52.46 months, and it was only 36.33 months in stage 4 (P corrected<0.0001, Log-rank test, figure 2G). The integrity of the tumor capsule was also correlated with RFS. Children with intact tumor capsules had a higher RFS (median: 62.60 months vs. 30.83 months, P <0.0001, Log-rank test, figure 2H). RFS was related to whether there was bone marrow metastasis at the initial onset. Children with bone marrow metastases had lower RFS (median: 21.05 months vs. 60.28 months, P<0.0001, Log-rank test, figure 2I). In summary, according to the univariate analysis, it can be concluded that the older onset age, with distant metastasis, bone marrow involvement, MYCN amplification, without intact tumor capsule, increased VMA and NSE value, and LDH>1000U/L were unfavorable factors for recurrence of NB. The univariate analysis data related to RFS was shown in Table 2.

2.2.4 Association Between MYCN and Relevant Laboratory Tests of NB

The study demonstrated the correlation between the MYCN gene and the data from the relevant laboratory tests. Children without MYCN amplification had a median initial VMA value of 2.03(0.48-35.89) mg/24h, and children with MYCN amplification had a median initial VMA value of 3.29(0.50-184.70) mg/24h (P=0.038). The median value of initial NSE in children without MYCN amplification was 74.55(12.79-1519.00) ug/l, and the median value of initial NSE with MYCN amplification was 244.00(12.90-4534.00) ug/l (P =0.031). It demonstrated that the MYCN gene was correlated with NSE and 24-hour urine VMA value at the initial onset. The correlation between the MYCN gene and other clinical features was listed in Table 3.

2.2.5 Multivariate Cox regression and nomogram

The significant variables in the univariate analysis included age at diagnosis, tumor localization, histologic category, bone marrow metastasis, MYCN state, the integrity of tumor capsule, VMA, NSE, and LDH value. The results from multivariate Cox regression analysis of RFS showed that onset age, tumor localization, MYCN state, histologic category, and integrity of tumor capsule were independent risk factors for RFS (all P values <0.05). The data of multivariate analysis were shown in Table 4.

Based on the multivariate analysis of RFS, all variables in the multivariate Cox regression were applied to build the nomogram model diagram for predicting RFS in the 1st-year, 5th-year, and 10th-year, as shown in Figure 3. Nomogram could assign points to each risk factor for recurrence, and finally, predict the RFS based on the total score individually. The corresponding meaning and description of the Arabic numbers in each risk factor in Figure 3 were shown in Table 5. For example,   if there were a 3-year-old patient with INSS Stage 4 at diagnosis, normal VMA and NES, LDH≤1000U/L, MYCN non-amplification, bone marrow MRD-negative, histologic category classified as undifferentiated neuroblastoma, and fracted tumor capsule, The total score was calculated as follows, 0 + 0 + 23 + 51 + 5 + 29 + 5 + 19 + 0 = 132 (scores), and the patient's 1st-year, 5th-year, and 10th-year recurrence-free probability was approximately 87%, 62%, and 45% referring to Figure 3, respectively.

Two methods were taken to verify the model: (1) used the Bootstrap method and repeated the sample 1000 times for internal verification. It showed that the recurrence prediction model had a C-index of 0.824 (P=0.023). (2) used the graph calibration method to verify the RFS of the 1st-year, 5th-year, and 10th-year RFS. See Figure 4 for details. It showed that the prediction curve of 1-year, 5-year, and 10-year was entangled with the standard curve, indicating that the model had good predictive potential.

Discussion

Neuroblastoma was one of the common extracranial solid tumors in childhood. The proportion of boys was larger in the analysis of clinical characteristics. However, gender was not the factor affecting RFS. It had been reported that boys had a higher incidence than girls, but the reason was not yet apparent, further research was needed (2). The relationship between onset age and time to recurrence was still unclear. This study concluded that onset age was an independent factor affecting RFS. The older children would be prone to earlier relapse. The prognosis of recurrent neuroblastoma was poor. Garaventa et al. (20) reported that the 10-year overall survival (OS) rate of relapsed patients was 14.4%, and age over 18 months was a poor prognostic factor. Most of the 71 relapsed cases in this cohort were 18 months and older, INSS stage 4, with bone marrow metastases. Therefore, the recurrence should be paid more attention to the first-onset patients with the clinical characteristics above.

More than half of children with neuroblastoma were accompanied by distant metastases at the first diagnosis, especially bone and bone marrow metastasis. It was consistent with the data of this study. According to Figure 1, the later MRD of bone marrow turned negative, the earlier children would relapse. The reason might be that if tumor cells in the bone marrow were more sensitive to chemotherapy, the tumor cells in the primary site were also sensitive to chemotherapy, and the treatment effect was better, and vice versa. It suggested that if patients developed bone marrow metastasis and positive MRD for a long time, it was necessary to increase the frequency of follow-up and shorten the time interval between follow-ups.

MYCN gene amplification was an essential prognostic factor for neuroblastoma. The 3-year OS of children with MYCN amplification was less than 30%. It had been reported that MYCN amplification was related to the primary abdomen, high LDH, massive tumor, poor pathological types, and chromosomes aberration (21). This study showed that 21.2% of children presented MYCN amplification, and the MYCN state was also an independent influencing factor for RFS. Children with MYCN amplification relapsed earlier, the reason might be that the MYCN amplification was positively related to tumor aggressiveness.

INPC proposed a prognostic classification based on age and histologic category, which was divided into favorable histology (FH) and unfavorable histology (UH)(16). GNBi of any age was classified as FH group, and GNBn of any age was in the UH group. Nakazawa et al. (22)verified that the prognosis of the FH group was better than the UH group (OS rates were 88.6% and 43.1%, respectively). Sokol E et al. (23)studied the influence of histologic category in INPC on the prognosis. The 5-year EFS of GNBi was greater than 85%, and the 5-year EFS of NB(differentiated ) was higher than that of NB(undifferentiated or poorly undifferentiated ). This study concluded that histologic category could be an independent factor for RFS. The RFS of GNBi was the longest, while the RFS of GNBn was the shortest, which was consistent with the trend of the above-mentioned prognostic analysis.

The tumor localization and tumor capsule were independent prognostic factors that affect RFS. Children with localized tumors and intact capsules had longer RFS. The reason might be that surgeons could completely remove those localized tumors with intact capsules. ZY Li et al. (24)also discussed that pediatric neuroblastomas in the head and neck were easier to complete resection if bulks with clear borders and intact capsules. So, these children's risks of recurrence were reduced. 

Other researchers had reported that the VMA value was correlated with MYCN amplification (20, 25, 26). Cangemi et al. (27) found that the ratio of urine VMA/HVA was an independent predictor of prognosis in patients with localized NB and MYCN non-amplification. LDH had an independent prognostic value in patients at all stages without MYCN amplification. In this study, MYCN amplification was correlated with the NSE value at the initial onset and 24-hour urine VMA value. It also showed that NSE value and 24-hour urine VMA value were significant predictors of recurrence in univariate analysis. Now in China, few centers could perform MYCN state testing for pediatric NB. Doctors might indirectly speculate about the MYCN state through these laboratory tests for children who had not been tested for MYCN state for any reason. If their NSE and 24-hour urine VMA values were abnormal, we could indicate that the MYCN gene amplification was likely to be positive, and the risk of recurrence was correspondingly increased. Therefore, these patients should be paid more attention to follow-ups after CR or VGPR. 

Researchers and members from major countries and international cooperative organizations in North America, Europe, and Asia had developed the International Neuroblastoma Risk Group (INRG) Classification and Staging System (INRGSS), which used preoperative radiological features and images-defined the risks Factors (IDRFs) to distinguish low-risk tumors from high-risk tumors(28, 29). It analyzed 13 clinical and biological variables on the impact of event-free survival, including two variables, LDH, and serum ferritin(30). This study also incorporated the two indicators and found that LDH value of more than 1000U/L was an important factor for RFS, while serum ferritin had no statistically significant effect on RFS (P=0.082). It might be due to the poor specificity of serum ferritin for NB. The laboratory tests concerning VMA, NES, and LDH had a critical influence on RFS in Univariate analysis. Further comparison of the three showed that the Hazard Ratio (HR) value was the highest in the NSE group (3.012 vs 5.742 vs 1.815) (see Figures 2A, 2B, and 2C for details). It had been reported that more than 90% of NB children with an abnormally increased NSE value on the first onset(31). Furthermore, Zelter(32) reported that NSE showed a significant correlation with INSS, patients with metastases had higher NSE values. So NSE value was of much more value for the early diagnosis and recurrence. For children with abnormal NSE values, we should shorten the follow-up interval, increase the frequency of follow-up, and closely monitor their recurrence.

The Nomogram model was accurate and concise. It could display the results graphically. It was widely used in clinical model prediction. Liang et al.(33) incorporated eight prognostic factors to build a Nomogram model to predict the OS rate of NB based on the SEER database. The applicability needed further verification for Chinese patients because Nomogram clinical prediction model was based on public databases, not Chinese patients, besides they did not target the recurrent NB patients. While this research had certain limitations. The dataset was limited to data from a single-center and of small size, which might be less convincing to demonstrate the effeteness and robustness of the proposed method. We conducted internal verification on the nomogram, but further external verification should have been determined whether it could be universally applied.

Conclusion

Increased VMA and NSE value, and LDH>1000U/L were important adverse factors for the recurrence of NB. NSE value was more valuable for predicting NB recurrence. Age at diagnosis, tumor localization, MYCN state, histologic category, and tumor capsule were independent risk factors for the recurrence of NB. The results demonstrated that the Nomogram could accurately predict the recurrence of children with neuroblastoma.

Declarations

Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Author Contributions

All authors contributed to the study conception and design. Data collection, follow-up, and analysis were performed by Quan Sun, Yanmin Chen, Qianya Jin, and Xiaojun Yuan. The first draft of the manuscript was written by Quan Sun and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Acknowledgments

The authors wish to thank the patients and the staff in the Department of Pediatric Hematology/Oncology, Xinhua Hospital Affiliated to Medicine School of Shanghai Jiaotong University.

Ethics Statement

The ethics approval was granted by the ethics committee of Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. This study was conducted in accordance with the principles of the Decla-ration of Helsinki.

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Tables

Table 1. Clinical Characteristics of Neuroblastoma

Factors

No.%

Factors

No.%

gender

 

VMAmg/24h

 

Male

131(61.8)

Normal

118(55.7)

Female

81 (38.2)

Abnormal

94(44.3)

agemonths

 

NSE (ug/l)

 

≤12

40(18.9)

Normal

13(6.1)

12~18

23(10.8)

Abnormal

199(93.9)

≥18

149(70.3)

LDH(U/L)

 

INSS

 

>1000

42(19.8)

1

8(3.8)

≤1000

170(80.2)

2

32(15.1)

Serum ferritin (ug/l)

 

3

60(28.3)

Normal

62(29.2)

4

106(50)

Abnormal

150(70.8)

4s

6(2.8)

MYCN state

 

Primary location

 

Non-amplification

125(58.9)

retroperitoneum/ paranephros

166(78.3)

amplification

45(21.2)

mediastinum

33(15.6)

NA

42(19.9)

Neck

5(2.4)

histologic category

 

Vertebra

4(1.9)

 

NB(undifferentiated or poorly undifferentiated )

 

121(57.1)

 

pelvis/ sacrococcyx

4(1.9)

NB(differentiated )

37(17.5)

Metastasis location

 

GNBi

45(21.2)

None

63(29.7)

GNBn

9(4.2)

Bone

89(42)

Tumor capsule

 

 lymph node

29(13.7)

Intact

133(62.7)

Multiple metastases*

37(17.5)

Fracted 

77(36.3)

Bone marrow

82(38.7)

NA (without surgery)

2(0.9)

Others

14(6.6)

Tumor sizescm

 

Chief complaint

 

≤5

96(45.3)

Physical examination

57(26.9)

5.1-9.9

106(50.0)

Asymptomatic mass

49(23.1)

≥10

10(4.7)

Digestive system symptoms

59(27.8)

recurrence

 

Respiratory symptoms

10(4.7)

Yes

71(33.5)

fever

14(6.6)

No

141(66.5)

Motor symptoms

21(9.9)

 

 

others

2(0.9)

 

 

*Multiple metastases*(≥3 metastases)

The normal value of VMA: <2mg/24h (<2 years old), <5mg/24h (2~18 years old); the normal value of NSE: 0-16.3ng/ml; the normal value of serum ferritin: 20-290ug/l.

Abbreviation:NB: neuroblastoma; GNBi: Ganglioneuroblastoma intermixed; GNBn: Ganglioneuroblastoma nodular; MRD: Minimal residual disease; LDH: lactate dehydrogenase; NES: Neuron-specific enolase; VMA: Vanilmandelic acid

Table 2. univariate analysis of RFS in recurrent Neuroblastoma

factors

 

Recurrence

 (no.)

non-recurrence

(no.)

median RFS

m)

P

HR*

95%CI

gender

male

47

84

39.53

0.302

 

 

female

24

57

48.20

 

 

Age (months)

≤12

4

36

62.30

<0.001

 

 

12~18

5

18

59.77

2.306

0.587-9.060

≥18

62

87

36.33

2.096

1.061-4.141

INSS

1,2,4s

2

44

90.67

<0.001

 

 

3

9

51

52.46

4.109

1.259-13.41

4

60

46

21.05

5.275

3.280-8.484

VMA(mg/24h)

normal

24

94

60.28

<0.001

3.012

1.871-4.849

increased

47

47

28.95

NSE (ug/l)

normal

1

12

91.33

0.048

5.742

2.385-13.830

increased

70

129

40.93

LDH(U/L)

≤1000

51

119

45.70

0.02

1.815

0.983-3.336

>1000

20

22

32.93

Serum Ferritin(ug/l)

normal

56

94

40.62

0.082

 

 

increased

15

47

52.23

 

 

MYCN state

Non-amplification

36

89

42.23

0.017

1.872

1.017-3.466

amplification

21

24

34.03

histologic category

NB(undifferentiated or poorly undifferentiated )

47

74

37.63

0.023

 

 

NB(differentiated )

14

23

49.73

GNBi

6

39

57.47

GNBn

4

5

32.60

tumor capsule

Intact

31

102

62.60

<0.001

2.651

1.696-4.378

fracted

39

38

30.83

tumor sizes(cm)

≤5

32

64

43.19

0.095

 

 

5.1-9.9

33

73

45.7

 

 

≥10

6

4

22.87

 

 

MRD in bone marrow

Negative/non-metastasis

24

92

60.28

<0.001

3.551

2.173-5.802

Positive /metastasis

46

36

21.05











Abbreviation:NB: neuroblastoma; GNBi: Ganglioneuroblastoma intermixed; GNBn: Ganglioneuroblastoma nodular; MRD: Minimal residual disease; LDH: lactate dehydrogenase; NES: Neuron-specific enolase; VMA: Vanilmandelic acid

The normal value of VMA: <2mg/24h (<2 years old), <5mg/24h (2~18 years old); the normal value of NSE: 0-16.3ng/ml; the normal value of serum ferritin: 20-290ug/l.

*HR denotes increased risk of the event for the second row within the given category as compared with the first row.

Table 3. Correlation between MYCN amplification and clinical features of neuroblastoma

 

factors

MYCN non-amplification

MYCN amplification

P

No.

Median(range)

No.

Median(range)

 

VMA(mg/24h)

125

2.03(0.48-35.89)

45

3.29(0.50-184.70)

0.038

NSE (ug/l)

125

74.55(12.79-1519.00)

45

244.00(12.90-4534.00)

0.031

LDH(U/L)

125

393.00(18.50-3966.00)

45

417.00(18.50-5425.00)

0.07

Serum ferritin(ug/l)

125

105.00(34.90-1710.00)

45

108.00(18.60-1010.00)

0.665








Abbreviation: LDH: lactate dehydrogenase; NES: Neuron-specific enolase; VMA: Vanilmandelic acid

 

Table 4. Multivariate analysis of RFS in recurrent neuroblastoma

factors

 

HR

95%CI

p-value

VMA

increased/normal

1.154

0.605-2.201

0.663

NSE

increased/normal

1.092

0.136-8.795

0.934

LDH

>1000/≤1000

1.022

0.535-1.954

0.947

MYCN amplification

with/without

1.948

1.075-3.530

0.028

Bone marrow metastasis

no/yes

0.716

0.378-1.356

0.305

Age(months)

≤12

*

*

0.037

 

12-18

2.854

0.560-14.544

0.207

 

≥18

4.682

1.411-15.536

0.012

INSS

1、2、4s

*

*

0.002

 

3

3.163

0.375-26.649

0.29

 

4

16.368

2.075-132.286

0.009

Histologic category

NB(undifferentiated or poorly undifferentiated )

*

*

0.007

 

NB(differentiated )

2.179

1.067-4.451

0.033

 

GNBi

0.325

0.123-0.856

0.023

 

GNBn

2.416

0.493-11.845

0.277

tumor capsule

intact/fracted

3.313

1.68-6.612

0.001

Abbreviation:NB: neuroblastoma; GNBi: Ganglioneuroblastoma intermixed; GNBn: Ganglioneuroblastoma nodular; MRD: Minimal residual disease; LDH: lactate dehydrogenase; NES: Neuron-specific enolase; VMA: Vanilmandelic acid

The normal value of VMA: <2mg/24h (<2 years old), <5mg/24h (2~18 years old); the normal value of NSE: 0-16.3ng/ml; the normal value of serum ferritin: 20-290ug/l.

HR denotes increased risk of the event for the second row within the given category as compared with the first row.

*Age≤12months, INSS stage1、2、4s, and pathological type NB(undifferentiated or poorly undifferentiated ) was the reference level.

Table 5. the score of each risk factor for recurrence in the nomogram model

factors

representatives

score

Age(m)

≤12

35

 

12~18

20

 

≥18

0

INSS

stage1、2、4s

100

 

stage3

58

 

stage4

0

VMA

normal

23

 

Increased

0

NSE

normal

51

 

Increased

0

LDH(U/L)

≤1000

5

 

>1000

0

MYCN

nonamplification

29

 

amplification

0

MRD in bone marrow

Negative/non-metastasis

0

 

Positive /metastasis

5

Pathological type

GNBn

0

(Histologic category)

NB(undifferentiated or poorly undifferentiated )

19

 

NB(differentiated )

31

 

GNBi

68

Tumor capsule

Fracted

0

 

Intact

41

Abbreviation:NB: neuroblastoma; GNBi: Ganglioneuroblastoma intermixed; GNBn: Ganglioneuroblastoma nodular; MRD: Minimal residual disease; LDH: lactate dehydrogenase; NES: Neuron-specific enolase; VMA: Vanilmandelic acid

The normal value of VMA: <2mg/24h (<2 years old), <5mg/24h (2~18 years old); the normal value of NSE: 0-16.3ng/ml.