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Research article
Wenjun Jiang
Sichuan Academy of Medical Sciences and Sichuan People's Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3400-3536
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
This article focuses on the roles and mechanism of lncRNA CRNDE on the progression of HCC.
Methods
We used qRT-PCR to detect the expression of lncRNA CRNDE in HCC cells, normal cells and clinical tissues. MTT assay, FCM analysis, Transwell migration and invasion assay were used to detect the effects of lncRNA CRNDE on cell viability, apoptosis, migration and invasion of HCC cells. The expression of apoptosis-related proteins Bcl-2, Bax, Cleaved Caspase 3, Cleaved Caspase 9, EMT epithelial marker E-cadherin and mesothelial marker Vimentin were analyzed by Western blot. Online prediction software was used to predict the binding sites between lncRNA CRNDE and miR-539-5p, or miR-539-5p and POU2F1 3’UTR. Dual luciferase reporter assay, qRT-PCR and RNA pulldown were used to detect target-relationship between lncRNA CRNDE and miR-539-5p. Dual luciferase reporter assay, qRT-PCR, Western blot and Immunofluorescence were used to detect target-relationship between miR-539-5p and POU2F1. qRT-PCR was used to detect the expression of miR-539-5p and POU2F1 in clinical tissues. Rescue experiments was used to evaluate the association among lncRNA CRNDE, miR-539-5p and POU2F1. Finally, we used Western blot to detect the effects of lncRNA CRNDE, miR-539-5p and POU2F1 on NF-κB and AKT pathway.
Results
lncRNA CRNDE was highly expressed in HCC cells and HCC tissues compared with normal cells and the corresponding adjacent normal tissues. lncRNA CRNDE promoted the cell viability, migration and invasion of HCC cells, while inhibited the apoptosis and promoted the EMT process of HCC cells. lncRNA CRNDE adsorbed miR-539-5p acts as a competitive endogenous RNA to regulate POU2F1 expression indirectly. In HCC clinical tissues, miR-539-5p expression decreased and POU2F1 increased compared with the corresponding adjacent normal tissues. lncRNA CRNDE/miR-539-5p/POU2-F1 participated the NF-κB and AKT pathway in HCC.
Conclusion
lncRNA CRNDE promotes the expression of POU2F1 by adsorbing miR-539-5p, thus promoting the progression of HCC.
Keywords: liver cancer, lncRNA CRNDE, miR-539-5p, POU2F1, ceRNA
Keywords
liver cancer, lncRNA CRNDE, miR-539-5p, POU2F1, ceRNA
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Fig5DNFkB.jpg
- Fig5ApIKB.jpg
- Fig5BAKT.jpg
- Fig5BpAKT.jpg
- Fig5CERK.jpg
- Fig5CpERK.jpg
- Fig5DGAPDH.jpg
- Fig3JPOU2F1.jpg
- Fig4BGAPDH.jpg
- Fig4BPOU2F1.jpg
- Fig5AIKB.jpg
- Fig2FECadherin.jpg
- Fig2FVimentin.jpg
- Fig3IGAPDH.jpg
- Fig3IPOU2F1.jpg
- Fig3JGAPDH.jpg
- Fig2CCleavedCaspase3.jpg
- Fig2FGAPDH.jpg
- Fig2CBcl2.jpg
- Fig2CCaspase3.jpg
- Fig2CGAPDH.jpg
- Fig2CBax.jpg
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CURRENT STATUS: Published
View the published article at BMC Cancer.
No community comments so far
Submission checks complete
On 19 Mar, 2020
Editorial decision: Accept
On 19 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 10 Mar, 2020
Reviewers invited
Invitations sent on 09 Mar, 2020
Reviewer #1 agreed
On 09 Mar, 2020
Review #1 received
Received 09 Mar, 2020
Editor assigned
On 06 Mar, 2020
Submission checks complete
On 05 Mar, 2020
Editor invited
On 05 Mar, 2020
Version 1
Posted 03 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 25 Feb, 2020
Editor assigned
On 24 Feb, 2020
Reviewers invited
Invitations sent on 24 Feb, 2020
Reviewer #1 agreed
On 24 Feb, 2020
Review #1 received
Received 24 Feb, 2020
Submission checks complete
On 23 Feb, 2020
Editor invited
On 23 Feb, 2020
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Comments: 0
PDF Downloads: ...
HTML Views: ...
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Cancer.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Wenjun Jiang
Sichuan Academy of Medical Sciences and Sichuan People's Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3400-3536
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Cancer.
No community comments so far
Submission checks complete
On 19 Mar, 2020
Editorial decision: Accept
On 19 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 10 Mar, 2020
Reviewers invited
Invitations sent on 09 Mar, 2020
Reviewer #1 agreed
On 09 Mar, 2020
Review #1 received
Received 09 Mar, 2020
Editor assigned
On 06 Mar, 2020
Submission checks complete
On 05 Mar, 2020
Editor invited
On 05 Mar, 2020
Version 1
Posted 03 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 25 Feb, 2020
Editor assigned
On 24 Feb, 2020
Reviewers invited
Invitations sent on 24 Feb, 2020
Reviewer #1 agreed
On 24 Feb, 2020
Review #1 received
Received 24 Feb, 2020
Submission checks complete
On 23 Feb, 2020
Editor invited
On 23 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Cancer.
Background
This article focuses on the roles and mechanism of lncRNA CRNDE on the progression of HCC.
Methods
We used qRT-PCR to detect the expression of lncRNA CRNDE in HCC cells, normal cells and clinical tissues. MTT assay, FCM analysis, Transwell migration and invasion assay were used to detect the effects of lncRNA CRNDE on cell viability, apoptosis, migration and invasion of HCC cells. The expression of apoptosis-related proteins Bcl-2, Bax, Cleaved Caspase 3, Cleaved Caspase 9, EMT epithelial marker E-cadherin and mesothelial marker Vimentin were analyzed by Western blot. Online prediction software was used to predict the binding sites between lncRNA CRNDE and miR-539-5p, or miR-539-5p and POU2F1 3’UTR. Dual luciferase reporter assay, qRT-PCR and RNA pulldown were used to detect target-relationship between lncRNA CRNDE and miR-539-5p. Dual luciferase reporter assay, qRT-PCR, Western blot and Immunofluorescence were used to detect target-relationship between miR-539-5p and POU2F1. qRT-PCR was used to detect the expression of miR-539-5p and POU2F1 in clinical tissues. Rescue experiments was used to evaluate the association among lncRNA CRNDE, miR-539-5p and POU2F1. Finally, we used Western blot to detect the effects of lncRNA CRNDE, miR-539-5p and POU2F1 on NF-κB and AKT pathway.
Results
lncRNA CRNDE was highly expressed in HCC cells and HCC tissues compared with normal cells and the corresponding adjacent normal tissues. lncRNA CRNDE promoted the cell viability, migration and invasion of HCC cells, while inhibited the apoptosis and promoted the EMT process of HCC cells. lncRNA CRNDE adsorbed miR-539-5p acts as a competitive endogenous RNA to regulate POU2F1 expression indirectly. In HCC clinical tissues, miR-539-5p expression decreased and POU2F1 increased compared with the corresponding adjacent normal tissues. lncRNA CRNDE/miR-539-5p/POU2-F1 participated the NF-κB and AKT pathway in HCC.
Conclusion
lncRNA CRNDE promotes the expression of POU2F1 by adsorbing miR-539-5p, thus promoting the progression of HCC.
Keywords: liver cancer, lncRNA CRNDE, miR-539-5p, POU2F1, ceRNA
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Fig5DNFkB.jpg
- Fig5ApIKB.jpg
- Fig5BAKT.jpg
- Fig5BpAKT.jpg
- Fig5CERK.jpg
- Fig5CpERK.jpg
- Fig5DGAPDH.jpg
- Fig3JPOU2F1.jpg
- Fig4BGAPDH.jpg
- Fig4BPOU2F1.jpg
- Fig5AIKB.jpg
- Fig2FECadherin.jpg
- Fig2FVimentin.jpg
- Fig3IGAPDH.jpg
- Fig3IPOU2F1.jpg
- Fig3JGAPDH.jpg
- Fig2CCleavedCaspase3.jpg
- Fig2FGAPDH.jpg
- Fig2CBcl2.jpg
- Fig2CCaspase3.jpg
- Fig2CGAPDH.jpg
- Fig2CBax.jpg
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