2.1 Clinical data
A retrospective analysis of 45 cervical cancer patients admitted to our hospital from January 2020 to December 2021 was used as the observation group, and 45 healthy cervical cancer patients in our hospital during the same period were selected as the control group. Inclusion criteria: ①All patients were pathologically diagnosed and met the diagnostic criteria of "Obstetrics and Gynecology" ; ②No radiotherapy and chemotherapy before surgery; ③All women were married women of childbearing age . Exclusion criteria: ① Pregnant or lactating women; ② Abnormal coagulation mechanism; ③ Combined with other malignant tumors . Observation group : aged 35-64 years old, average (43.69 ± 5.41 ) years old; pregnancy 0-4 times, average (2.05 ± 0.32 ) times; parity 0-3 times, average (1.25 ± 0.21 ) times. Control group: aged 34-64 years, mean (43.34 ± 5.32 ) years old; 0-4 times of pregnancy, mean (2.11 ± 0.34 ) times; parity 0-3 times, mean (1.22 ± 0.25 ) times. There was no significant difference in clinical characteristics between the two groups ( P > 0.05)
2.2 Experimental method
All tissue specimens were embedded in paraffin for serial sections with a thickness of 4 μm, and stained by immunohistochemical streptavidin-biotin complex method (SABC method) and diaminobenzidine (DAB) staining. KGFR, TFPI-2 protein universal secondary antibody, SP kit and DAB color development kit were purchased from Guangzhou Jinwen Biotechnology Co., Ltd. Blind reading was performed by two pathologists, and 5 fields of view were randomly selected for interpretation under a 200x microscope. The brownish yellow cytoplasm could be positive for TFPI-2 expression. The positive films and PBS were used instead of primary antibodies as positive and negative controls, respectively, and the expression of KGFR and TFPI-2 proteins was observed under a high-power microscope. The specific staining steps were operated in strict accordance with the SP kit instructions. 5 fields of view were randomly selected, and 100 cells were counted in each field of view. If the cytoplasm appeared brown, the expression of KGFR or TFPI-2 was positive. The proportion of positive cells in the total number of cells was ≥30%, which was positive expression, otherwise it was negative expression.
Statements：①All methods were carried out in accordance with relevant guidelines and regulations；②All experimental protocols were approved by H Hunan Vocational and Technical College of Environmental Biology IRB committee;③This study is obtained from all subjects and/or their legal guardian(s) and written informed consent obtained from obtained all subjects and/or their legal guardian(s).④All data generated or analysed during this study are included in its supplementary information files.
2.3 Observation indicators
positive expressions of KGFR and TFPI-2 in the two groups were recorded , the expressions of KGFR and TFPI-2 and the clinicopathological characteristics of cervical cancer, as well as the correlation between the two and cervical cancer.
2.4 Statistical methods
SPSS 23.0 statistical software was used for statistical analysis of the data. Measurement data were expressed as mean ± standard deviation ( x¯ ±s), enumeration data were expressed as n (%), χ 2 test was used , and logistic was used for correlation analysis .