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Research article
Jesus K. Yamamoto-Furusho
Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5247-5812
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Background: Patients clinical phenotypes in gastroesophageal reflux disease (GERD) are classified as: Barrett's esophagus (BE), erosive esophagitis (EE) and non-erosive gastroesophageal reflux disease (NERD). The aim of this study was to characterize genes involved in the pathophysiology and immune response of GERD.
Methods: This is an observational and cross-sectional study. All patients with BE, EE, NERD and the control group were subjected to a superior endoscopy (with biopsies of esophageal mucosa). The cytokine mRNA relative quantification of target genes was conducted by RT-PCR. Changes in gene expression were assessed of the genes associated with inflammation in each disease phenotype. Statistical analysis of differential gene expression was performed by using Dunn's Test for Multiple Comparisons. A p value < 0.05 was considered as significant.
Results: A Total of 98 patients were included and they were divided into the following groups: Group BE 16 (16.33%), Group EE 23 (23.47%), Group NERD 42 (42.86%) and Control Group 17 (17.35%). When comparing with control group we found: patients with BE showed an increased expression of IL-8 (P<0.005) and higher levels of: IL-1β, NF-κβ, IL-10 and MMP-3, MMP-9 as well; patients with EE had higher levels of IL-1B, IL-6, IL-8 and IL-10 (P<0.005) and patients with NERD showed a differential gene expression of cytokines Th1, particularly TNF-α and IL-1B (P<0.005) and decreased gene expression of Th2 cytokines such as IL-10, IL-8 and MMP9.
Conclusions: This study demonstrates the differential expression of mediators of inflammation in the esophageal mucosa of patients with the EE and BE phenotype.
Keywords
Esophagitis, Non-Erosive Reflux Disease (Nerd), Cytokine, Gene
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CURRENT STATUS: Published
View the published article at BMC Gastroenterology.
No community comments so far
Editorial decision: Major revision
On 27 Jan, 2021
Review #1 received
Received 10 Jan, 2021
Reviewers invited
Invitations sent on 03 Jan, 2021
Reviewer #1 agreed
On 03 Jan, 2021
Editor assigned
On 25 Nov, 2020
Submission checks complete
On 25 Nov, 2020
Editor invited
On 25 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 27 Oct, 2020
Review #2 received
Received 07 Oct, 2020
Review #1 received
Received 07 Oct, 2020
Reviewers invited
Invitations sent on 06 Oct, 2020
Reviewer #1 agreed
On 06 Oct, 2020
Reviewer #2 agreed
On 06 Oct, 2020
Editor assigned
On 01 Sep, 2020
Submission checks complete
On 31 Aug, 2020
Editor invited
On 31 Aug, 2020
Version 1
Posted 05 Mar, 2020
No comments provided
Editorial decision: Major revision
On 10 Aug, 2020
Review #3 received
Received 21 Jul, 2020
Reviewer #3 agreed
On 15 Jul, 2020
Review #2 received
Received 23 May, 2020
Reviewer #2 agreed
On 18 May, 2020
Review #1 received
Received 01 May, 2020
Reviewers invited
Invitations sent on 14 Apr, 2020
Reviewer #1 agreed
On 14 Apr, 2020
Editor assigned
On 24 Feb, 2020
Submission checks complete
On 23 Feb, 2020
Editor invited
On 23 Feb, 2020
First submitted
On 21 Feb, 2020
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Subject Areas
Gastroenterology & Hepatology
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See the published version of this preprint at BMC Gastroenterology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Jesus K. Yamamoto-Furusho
Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5247-5812
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Gastroenterology.
No community comments so far
Editorial decision: Major revision
On 27 Jan, 2021
Review #1 received
Received 10 Jan, 2021
Reviewers invited
Invitations sent on 03 Jan, 2021
Reviewer #1 agreed
On 03 Jan, 2021
Editor assigned
On 25 Nov, 2020
Submission checks complete
On 25 Nov, 2020
Editor invited
On 25 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 27 Oct, 2020
Review #2 received
Received 07 Oct, 2020
Review #1 received
Received 07 Oct, 2020
Reviewers invited
Invitations sent on 06 Oct, 2020
Reviewer #1 agreed
On 06 Oct, 2020
Reviewer #2 agreed
On 06 Oct, 2020
Editor assigned
On 01 Sep, 2020
Submission checks complete
On 31 Aug, 2020
Editor invited
On 31 Aug, 2020
Version 1
Posted 05 Mar, 2020
No comments provided
Editorial decision: Major revision
On 10 Aug, 2020
Review #3 received
Received 21 Jul, 2020
Reviewer #3 agreed
On 15 Jul, 2020
Review #2 received
Received 23 May, 2020
Reviewer #2 agreed
On 18 May, 2020
Review #1 received
Received 01 May, 2020
Reviewers invited
Invitations sent on 14 Apr, 2020
Reviewer #1 agreed
On 14 Apr, 2020
Editor assigned
On 24 Feb, 2020
Submission checks complete
On 23 Feb, 2020
Editor invited
On 23 Feb, 2020
First submitted
On 21 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Gastroenterology & Hepatology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Gastroenterology.
Background: Patients clinical phenotypes in gastroesophageal reflux disease (GERD) are classified as: Barrett's esophagus (BE), erosive esophagitis (EE) and non-erosive gastroesophageal reflux disease (NERD). The aim of this study was to characterize genes involved in the pathophysiology and immune response of GERD.
Methods: This is an observational and cross-sectional study. All patients with BE, EE, NERD and the control group were subjected to a superior endoscopy (with biopsies of esophageal mucosa). The cytokine mRNA relative quantification of target genes was conducted by RT-PCR. Changes in gene expression were assessed of the genes associated with inflammation in each disease phenotype. Statistical analysis of differential gene expression was performed by using Dunn's Test for Multiple Comparisons. A p value < 0.05 was considered as significant.
Results: A Total of 98 patients were included and they were divided into the following groups: Group BE 16 (16.33%), Group EE 23 (23.47%), Group NERD 42 (42.86%) and Control Group 17 (17.35%). When comparing with control group we found: patients with BE showed an increased expression of IL-8 (P<0.005) and higher levels of: IL-1β, NF-κβ, IL-10 and MMP-3, MMP-9 as well; patients with EE had higher levels of IL-1B, IL-6, IL-8 and IL-10 (P<0.005) and patients with NERD showed a differential gene expression of cytokines Th1, particularly TNF-α and IL-1B (P<0.005) and decreased gene expression of Th2 cytokines such as IL-10, IL-8 and MMP9.
Conclusions: This study demonstrates the differential expression of mediators of inflammation in the esophageal mucosa of patients with the EE and BE phenotype.
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
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