Aging-related impairment of the blood brain barrier (BBB) and neurovascular unit (NVU) increases the risk for neurodegeneration. Among various cells that participate in BBB and NVU function, calcium signals in astrocytic endfeet are crucial for BBB and NVU integrity. To assess if aging is associated with altered calcium signals within astrocytic endfeet of the dorsolateral striatum (DLS), we expressed a genetically encoded calcium indicator, GCaMP6f in DLS astrocytes of young (3-4 month) and aging (20-24 month) mice. Compared to young mice, DLS endfeet in aging mice demonstrated decreases in calreticulin expression, and significant alterations in membrane-associated and mitochondrial calcium signals. While young mice required both extracellular and endoplasmic reticulum calcium sources for endfoot signals, aging mice showed heavy dependence on endoplasmic reticulum calcium. Thus, aging astrocytic endfeet possess significant changes in calcium buffering and sources, which is important for understanding mechanisms by which aging can impair the BBB and NVU.