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Maheen Shamim
SZABIST: Shaheed Zulfikar Ali Bhutto Institute of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0104-5844
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Water quality assessment demands a precise anatomization of specimens that comply with acclaimed water purity standards. Today, the growing number of toxicants and their synergistic consequences make it necessary to develop general toxicity assays able to examine and determine water pollution. Contemporary general toxicity methods hinder specimen analysis due to their prolonged operation protocols. Also, the equipment involved is very expensive that not everyone can afford it. In an effort to resolve these drawbacks, a quick and cost effective toxicity bioassay based on chromatic changes related to bacterial ferricyanide reduction is introduced here. E.coli cells (Model Bacteria) were stably confined on four supports: Cellulose-based Paper Discs, Silica 60, Polystyrene, and Acrylic Beads, which remained useful for a long period at -20ºC. Copper was used as a model toxic agent to perform Bioassay Assessment. Chromatic changes related to bacterial ferricyanide reduction were determined by visual inspection. Cellulose Paper Discs, Polystyrene, and Acrylic beads showed good results and better viability, while Silica 60 proved itself as a weak support and resulted in poor viability.
Keywords
Bacterial ferricyanide reduction, Immobilization, Microbial Biosensor, Chromatic Assay, Heavy Metal Water Contamination
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Maheen Shamim
SZABIST: Shaheed Zulfikar Ali Bhutto Institute of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0104-5844
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 05 Feb, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Water quality assessment demands a precise anatomization of specimens that comply with acclaimed water purity standards. Today, the growing number of toxicants and their synergistic consequences make it necessary to develop general toxicity assays able to examine and determine water pollution. Contemporary general toxicity methods hinder specimen analysis due to their prolonged operation protocols. Also, the equipment involved is very expensive that not everyone can afford it. In an effort to resolve these drawbacks, a quick and cost effective toxicity bioassay based on chromatic changes related to bacterial ferricyanide reduction is introduced here. E.coli cells (Model Bacteria) were stably confined on four supports: Cellulose-based Paper Discs, Silica 60, Polystyrene, and Acrylic Beads, which remained useful for a long period at -20ºC. Copper was used as a model toxic agent to perform Bioassay Assessment. Chromatic changes related to bacterial ferricyanide reduction were determined by visual inspection. Cellulose Paper Discs, Polystyrene, and Acrylic beads showed good results and better viability, while Silica 60 proved itself as a weak support and resulted in poor viability.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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