COVID-19 is a highly contagious viral infection leading to many deaths worldwide and has created a challenge to the health care system of countries. Therefore, many studies have been conducted by researchers to identify viral infection, factors involved in the severity of the infection, and its mortality rate, and it continues to this day [6, 8]. The coincidence of other diseases, especially viral infections, could cause the exacerbation of the COVID-19 infection . In this study, we evaluated the rate of influenza virus and SARS-CoV-2 coinfection in people with symptoms of COVID-19 infection. We detected meager coinfection rates among patients (191 of 14,121 samples tested). The studies in China and USA showed a similarly low proportion of coinfection (0.4% and 0.9%, respectively) in their patient samples. The coinfection rate was low in this study, despite the high rate of a single infection, which may be due to competition between the influenza virus and SARS-CoV-2 [10, 11]. In the results of the meta-analysis study, related to Lansbury et al., it has been shown that the coinfections of COVID-19 with viral and bacterial were 3% and 7%, respectively, in patients . Although simultaneous infection with bacterial and viral agents can increase deaths in the SARSCoV2 infection, there is still insufficient evidence . One of the common symptoms that COVID-19 and influenza share is respiratory symptoms. The mechanism of virus transmission, clinical manifestations, and seasonal emergence of the COVID-19 are similar to those of the influenza virus. Therefore, the simultaneous infection of SARS-CoV2 and influenza can intervene with the recognition and therapy of patients. Especially in high-risk patients, this coinfection can increase the severity of the disease and even increase the risk of death [6, 9]. Both influenza virus and SARS-CoV-2 are airborne pathogens. The primary location for the proliferation of influenza virus is alveolar type II cells (AT2 pneumocytes), which the SARS-CoV-2 also preferably infects these cells. A consider a simultaneous infection of influenza and COVID-19; this can increase the side effects of SARS-CoV-2 infection and play a significant role in COVID-19 infection development [12, 13]. For this reason, seasonal influenza and COVID-19 pandemics could include large populations of people at risk of contracting both viruses at the same time . Regarding demographics, the people aged 19–60 years were more likely to get infected with SARS-CoV-2 and influenza infection. In reports from Bangladesh, the elderly aged > 60 years have been considered a significant risk factor for COVID-19 infection. In our study, males were more likely to be infected with influenza. Also, females were more likely to be infected with SARS-CoV-2 and coinfection pathogens than males. The findings from Italy  and Bangladesh  research showed males were more infected with SARS-CoV-2 than females. Our result showed the prevalence of influenza and SARS-CoV-2 coinfection was found to be higher in outpatient samples than in inpatients. This study has some limitations; we had no information about the patients' clinical symptoms, and we did not have a history of any underlying disease such as hypertension, diabetes, or use of the seasonal influenza vaccine in the individuals. In conclusion, our data propose that the influenza virus and SARS-CoV-2 were not very common in Hamadan, which shows an apparent absence of influenza virus circulation during the peak influenza season in Hamadan.