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Research article
Jian-ping Han
Corresponding Author
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Background
Fritillariae Cirrhosae Bulbus, which is a traditional antitussive and expectorant medicine in China, is derived from six original plants. Among these six species, all except for Fritillaria taipaiensis and Fritillaria unibracteata var. wabuensis have been recorded in the National Protected Plants III. Their major bioactive compounds, including imperialine and peimisine, are steroidal alkaloids, derived from the steroid synthesis pathway. However, research on the F. taipaiensis genome, transcriptomes and steroid-synthesis-related genes has been lacking for a long time. In this study, the first transcriptome sequencing of F. taipaiensis was performed.
Results
A total of 94,396,694 (~11.4 Gb) high-quality reads obtained using paired-end Illumina sequencing were de novo assembled into 190,350 transcripts. Functional annotation with multiple public databases identified an array of genes involved in steroidal alkaloid biosynthesis and other secondary metabolite pathways, including steroid and terpenoid backbone biosynthesis, and important oxidoreductases. The large number of differentially expressed transcripts together with CYPs suggests the involvement of these candidates in tissue-specific expression. The expression analysis revealed that bulbs may be the main site of the upstream steroidal alkaloid biosynthesis pathway, and we speculated that the downstream reactions, including the oxidation-reduction reaction catalyzed by CYPs, might also occur primarily in bulbs.
Conclusion
In conclusion, the comprehensive transcriptome dataset created in this study will serve as a resource for the identification of potential candidates for the genetic manipulation of targeted bioactive metabolites and will also contribute to the development of functionally relevant molecular marker resources to expedite molecular breeding and conservation efforts for F. taipaiensis.
Keywords
Fritillariae Cirrhosae Bulbus, Fritillaria taipaiensis, steroidal alkaloids, biosynthesis pathway, CYP450s
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Jian-ping Han
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 26 Jun, 2019
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Molecular Biology and Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Fritillariae Cirrhosae Bulbus, which is a traditional antitussive and expectorant medicine in China, is derived from six original plants. Among these six species, all except for Fritillaria taipaiensis and Fritillaria unibracteata var. wabuensis have been recorded in the National Protected Plants III. Their major bioactive compounds, including imperialine and peimisine, are steroidal alkaloids, derived from the steroid synthesis pathway. However, research on the F. taipaiensis genome, transcriptomes and steroid-synthesis-related genes has been lacking for a long time. In this study, the first transcriptome sequencing of F. taipaiensis was performed.
Results
A total of 94,396,694 (~11.4 Gb) high-quality reads obtained using paired-end Illumina sequencing were de novo assembled into 190,350 transcripts. Functional annotation with multiple public databases identified an array of genes involved in steroidal alkaloid biosynthesis and other secondary metabolite pathways, including steroid and terpenoid backbone biosynthesis, and important oxidoreductases. The large number of differentially expressed transcripts together with CYPs suggests the involvement of these candidates in tissue-specific expression. The expression analysis revealed that bulbs may be the main site of the upstream steroidal alkaloid biosynthesis pathway, and we speculated that the downstream reactions, including the oxidation-reduction reaction catalyzed by CYPs, might also occur primarily in bulbs.
Conclusion
In conclusion, the comprehensive transcriptome dataset created in this study will serve as a resource for the identification of potential candidates for the genetic manipulation of targeted bioactive metabolites and will also contribute to the development of functionally relevant molecular marker resources to expedite molecular breeding and conservation efforts for F. taipaiensis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
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