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Fenghe Zhang
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Background : The development of an ideal implant material with appropriate antibacterial properties and that improves osteogenesis is essential for the guidance of new bone formation in orthopedic and tooth implant surgeries. In this study, we developed minocycline-modified pure titanium.
Methods: We exploited the chemistry of polydopamine (PD) for the coating of minocycline. PD was coated on pure titanium, on which minocycline was subsequently immobilized under certain conditions. Minocycline coating was verified by characterizing the surface chemical composition of the coated Ti sheet and was quantitatively measured by fluorescamine assay.
Results: The minocycline-coated pure titanium showed a lower bacterial adhesion rate and supported the spread of the osteogenesis differentiation of human mesenchymal stem cells (hMSCs). A remarkable increase in alkaline phosphatase activity and calcium deposition was found when hMSCs were cultured on minocycline-coated pure titanium for 28 days.
Conclusions: The minocycline-coated pure titanium may be optimized as clinically applicable bioactive materials for implant and bone materials.
Keywords
Minocycline-Modified Pure Titanium, Antibacterial Properties, Osteogenesis, Bone Marrow Stem Cells
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Fenghe Zhang
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 10 Mar, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Stem Cell & Developmental Cell Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background : The development of an ideal implant material with appropriate antibacterial properties and that improves osteogenesis is essential for the guidance of new bone formation in orthopedic and tooth implant surgeries. In this study, we developed minocycline-modified pure titanium.
Methods: We exploited the chemistry of polydopamine (PD) for the coating of minocycline. PD was coated on pure titanium, on which minocycline was subsequently immobilized under certain conditions. Minocycline coating was verified by characterizing the surface chemical composition of the coated Ti sheet and was quantitatively measured by fluorescamine assay.
Results: The minocycline-coated pure titanium showed a lower bacterial adhesion rate and supported the spread of the osteogenesis differentiation of human mesenchymal stem cells (hMSCs). A remarkable increase in alkaline phosphatase activity and calcium deposition was found when hMSCs were cultured on minocycline-coated pure titanium for 28 days.
Conclusions: The minocycline-coated pure titanium may be optimized as clinically applicable bioactive materials for implant and bone materials.
Figure 1
Figure 2
Figure 3
Figure 4
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